Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5446 and Pf1N1B4_5447 are separated by 46 nucleotides
Pf1N1B4_5446 - hypothetical protein, at 192,136 to 192,315
_5446
Pf1N1B4_5447 - hypothetical protein, at 192,362 to 192,556
_5447
Position (kb)
192
193 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 191.430 kb on + strand at 191.432 kb on + strand at 191.433 kb on - strand at 191.478 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.537 kb on + strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.538 kb on - strand at 191.541 kb on + strand at 191.542 kb on - strand at 191.576 kb on - strand at 191.576 kb on - strand at 191.603 kb on - strand at 191.615 kb on + strand at 191.616 kb on - strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.628 kb on + strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.629 kb on - strand at 191.632 kb on + strand at 191.697 kb on + strand at 191.697 kb on + strand at 191.698 kb on - strand at 191.716 kb on + strand at 191.717 kb on - strand at 191.717 kb on - strand at 191.717 kb on - strand at 191.719 kb on + strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.720 kb on - strand at 191.737 kb on + strand at 191.738 kb on - strand at 191.781 kb on - strand at 191.802 kb on - strand at 191.802 kb on - strand at 191.802 kb on - strand at 191.815 kb on - strand at 191.815 kb on - strand at 191.815 kb on - strand at 191.836 kb on + strand at 191.837 kb on - strand at 191.837 kb on - strand at 191.851 kb on + strand at 191.852 kb on - strand at 191.881 kb on - strand at 191.892 kb on + strand at 191.893 kb on - strand at 191.893 kb on - strand at 191.893 kb on - strand at 191.906 kb on + strand at 191.906 kb on + strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.907 kb on - strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.927 kb on + strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 191.928 kb on - strand at 192.086 kb on - strand at 192.086 kb on - strand at 192.086 kb on - strand at 192.129 kb on - strand at 192.137 kb on + strand at 192.137 kb on + strand at 192.172 kb on - strand, within Pf1N1B4_5446 at 192.229 kb on + strand, within Pf1N1B4_5446 at 192.229 kb on + strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.230 kb on - strand, within Pf1N1B4_5446 at 192.249 kb on - strand, within Pf1N1B4_5446 at 192.387 kb on + strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.388 kb on - strand, within Pf1N1B4_5447 at 192.412 kb on + strand, within Pf1N1B4_5447 at 192.441 kb on - strand at 192.441 kb on - strand, within Pf1N1B4_5447 at 192.441 kb on - strand at 192.540 kb on + strand at 192.540 kb on + strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.541 kb on - strand at 192.551 kb on + strand at 192.552 kb on - strand at 192.552 kb on - strand at 192.571 kb on - strand at 192.573 kb on + strand at 192.574 kb on - strand at 192.574 kb on - strand at 192.635 kb on - strand at 192.671 kb on - strand at 192.671 kb on - strand at 192.671 kb on - strand at 192.677 kb on + strand at 192.677 kb on + strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.678 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.733 kb on - strand at 192.740 kb on + strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.741 kb on - strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.761 kb on + strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.762 kb on - strand at 192.853 kb on - strand at 192.853 kb on - strand at 192.853 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.863 kb on - strand at 192.934 kb on - strand at 192.934 kb on - strand at 192.945 kb on - strand at 192.945 kb on - strand at 192.945 kb on - strand at 193.010 kb on - strand at 193.066 kb on - strand at 193.066 kb on - strand at 193.123 kb on + strand at 193.146 kb on + strand at 193.147 kb on - strand at 193.147 kb on - strand at 193.170 kb on - strand at 193.170 kb on - strand at 193.208 kb on + strand at 193.209 kb on - strand at 193.209 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand at 193.267 kb on - strand at 193.329 kb on - strand at 193.329 kb on - strand at 193.329 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.335 kb on - strand at 193.340 kb on + strand at 193.341 kb on - strand at 193.341 kb on - strand at 193.341 kb on - strand at 193.369 kb on - strand at 193.383 kb on + strand at 193.399 kb on + strand at 193.449 kb on + strand at 193.450 kb on - strand at 193.552 kb on - strand at 193.552 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 191,430 + -2.3 191,432 + -0.7 191,433 - +1.1 191,478 + -0.9 191,537 + -0.4 191,537 + -1.5 191,537 + +2.4 191,537 + +0.7 191,537 + -1.9 191,537 + +0.1 191,537 + +0.2 191,538 - +0.6 191,538 - -1.5 191,538 - -0.5 191,538 - -0.6 191,538 - -0.9 191,538 - +1.1 191,541 + -1.9 191,542 - -0.3 191,576 - -0.5 191,576 - -0.4 191,603 - -0.9 191,615 + -1.4 191,616 - +0.6 191,628 + -0.9 191,628 + -0.5 191,628 + -1.5 191,628 + -0.5 191,628 + +0.4 191,628 + +0.7 191,628 + -1.5 191,629 - +0.1 191,629 - +0.1 191,629 - +0.1 191,629 - +0.1 191,632 + +0.1 191,697 + +0.5 191,697 + +0.1 191,698 - -0.7 191,716 + +0.9 191,717 - +0.5 191,717 - -1.8 191,717 - -1.2 191,719 + +0.4 191,720 - -0.4 191,720 - +0.2 191,720 - -0.9 191,720 - +0.1 191,720 - +0.1 191,737 + -1.5 191,738 - -0.9 191,781 - +0.1 191,802 - -1.2 191,802 - +0.1 191,802 - -0.9 191,815 - -0.9 191,815 - +0.4 191,815 - +0.1 191,836 + -1.9 191,837 - -0.9 191,837 - +1.1 191,851 + -2.3 191,852 - -0.5 191,881 - +1.1 191,892 + -0.2 191,893 - -1.9 191,893 - +0.6 191,893 - +1.5 191,906 + -0.5 191,906 + +0.1 191,907 - +0.1 191,907 - +1.1 191,907 - +0.6 191,907 - -0.4 191,927 + +0.1 191,927 + +2.1 191,927 + +1.5 191,927 + +0.7 191,927 + +0.1 191,927 + -0.5 191,928 - -0.4 191,928 - -1.5 191,928 - -0.3 191,928 - -1.2 191,928 - -0.2 191,928 - +0.6 191,928 - +0.1 191,928 - +1.3 191,928 - +1.6 191,928 - +2.4 191,928 - -0.4 191,928 - -0.9 192,086 - -2.8 192,086 - -0.2 192,086 - +0.5 192,129 - +1.1 192,137 + +0.1 192,137 + -0.5 192,172 - Pf1N1B4_5446 0.20 -2.3 192,229 + Pf1N1B4_5446 0.52 -0.5 192,229 + Pf1N1B4_5446 0.52 -0.2 192,230 - Pf1N1B4_5446 0.52 +0.1 192,230 - Pf1N1B4_5446 0.52 -0.5 192,230 - Pf1N1B4_5446 0.52 -0.9 192,249 - Pf1N1B4_5446 0.63 -2.3 192,387 + Pf1N1B4_5447 0.13 +1.1 192,388 - Pf1N1B4_5447 0.13 -0.9 192,388 - Pf1N1B4_5447 0.13 +1.9 192,388 - Pf1N1B4_5447 0.13 +2.1 192,388 - Pf1N1B4_5447 0.13 -0.9 192,412 + Pf1N1B4_5447 0.26 +0.5 192,441 - +0.1 192,441 - Pf1N1B4_5447 0.41 -0.5 192,441 - -0.5 192,540 + -1.1 192,540 + -0.9 192,541 - +0.1 192,541 - -0.9 192,541 - -0.3 192,541 - +0.6 192,541 - +0.1 192,551 + -1.9 192,552 - +0.3 192,552 - +0.6 192,571 - -0.7 192,573 + +0.1 192,574 - +0.4 192,574 - -0.8 192,635 - +0.4 192,671 - +0.1 192,671 - +0.1 192,671 - +1.1 192,677 + +1.1 192,677 + +0.3 192,678 - -1.5 192,678 - -0.4 192,678 - -1.1 192,678 - -1.5 192,678 - +0.5 192,678 - -0.4 192,678 - -0.9 192,678 - +1.0 192,678 - -1.4 192,678 - +0.4 192,678 - -1.3 192,678 - -0.2 192,678 - +0.8 192,678 - -0.1 192,678 - -1.4 192,678 - -0.5 192,678 - +0.2 192,678 - -0.6 192,678 - -0.4 192,678 - -1.9 192,733 - -0.8 192,733 - +0.1 192,733 - -0.3 192,733 - -1.4 192,740 + +0.1 192,741 - -1.4 192,741 - -0.5 192,741 - +0.4 192,741 - -0.4 192,741 - -0.8 192,761 + +1.2 192,761 + +1.9 192,761 + +0.1 192,761 + -1.3 192,762 - +0.6 192,762 - -0.9 192,762 - -0.9 192,762 - -0.9 192,762 - +0.1 192,762 - -0.9 192,762 - -0.1 192,762 - -0.6 192,762 - -2.3 192,762 - -0.7 192,762 - +0.6 192,762 - -0.2 192,762 - +0.3 192,762 - -1.2 192,762 - -1.2 192,762 - +1.2 192,762 - +1.4 192,762 - +0.7 192,762 - +1.1 192,762 - +0.6 192,762 - +0.1 192,853 - +1.6 192,853 - -0.6 192,853 - +0.1 192,863 - -1.8 192,863 - -0.9 192,863 - -2.5 192,863 - +1.6 192,863 - -0.4 192,863 - +0.1 192,863 - -1.4 192,863 - -0.4 192,863 - -0.4 192,863 - +0.4 192,863 - +0.6 192,934 - +0.3 192,934 - +0.1 192,945 - -0.5 192,945 - -1.8 192,945 - +0.5 193,010 - +0.6 193,066 - +0.1 193,066 - +1.1 193,123 + +0.1 193,146 + -0.2 193,147 - +0.5 193,147 - -1.5 193,170 - -0.8 193,170 - +2.1 193,208 + +0.2 193,209 - -0.9 193,209 - +1.1 193,267 - +1.1 193,267 - +0.5 193,267 - -1.3 193,267 - -0.6 193,329 - -0.3 193,329 - -0.9 193,329 - +1.1 193,335 - +0.6 193,335 - +0.8 193,335 - +0.1 193,335 - -1.5 193,335 - +0.1 193,335 - +0.1 193,335 - +0.8 193,335 - +0.1 193,335 - +0.6 193,340 + -0.5 193,341 - -1.5 193,341 - -0.9 193,341 - -0.9 193,369 - -0.9 193,383 + -0.6 193,399 + -1.5 193,449 + -0.2 193,450 - -0.4 193,552 - -0.4 193,552 - -1.5
Or see this region's nucleotide sequence