Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5434 and Pf1N1B4_5435 are separated by 704 nucleotides Pf1N1B4_5435 and Pf1N1B4_5436 are separated by 700 nucleotides
Pf1N1B4_5434 - HIPA PROTEIN, at 173,694 to 175,007
_5434
Pf1N1B4_5435 - hypothetical protein, at 175,712 to 175,990
_5435
Pf1N1B4_5436 - Probable helicase, at 176,691 to 178,637
_5436
Position (kb) 175
176 Strain fitness (log2 ratio) -2
-1
0
1
2 at 174.730 kb on + strand, within Pf1N1B4_5434 at 174.731 kb on - strand, within Pf1N1B4_5434 at 174.744 kb on + strand, within Pf1N1B4_5434 at 174.762 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.827 kb on + strand, within Pf1N1B4_5434 at 174.911 kb on + strand at 174.911 kb on + strand at 174.911 kb on + strand at 174.911 kb on + strand at 174.911 kb on + strand at 174.912 kb on - strand at 174.927 kb on + strand at 175.005 kb on + strand at 175.005 kb on + strand at 175.005 kb on + strand at 175.005 kb on + strand at 175.050 kb on + strand at 175.114 kb on + strand at 175.115 kb on - strand at 175.148 kb on + strand at 175.157 kb on + strand at 175.157 kb on + strand at 175.157 kb on + strand at 175.158 kb on - strand at 175.158 kb on - strand at 175.173 kb on + strand at 175.181 kb on + strand at 175.182 kb on - strand at 175.312 kb on + strand at 175.354 kb on + strand at 175.407 kb on + strand at 175.407 kb on + strand at 175.408 kb on - strand at 175.431 kb on - strand at 175.613 kb on + strand at 175.614 kb on - strand at 175.691 kb on + strand at 175.751 kb on + strand, within Pf1N1B4_5435 at 175.751 kb on + strand, within Pf1N1B4_5435 at 175.968 kb on + strand at 176.196 kb on + strand at 176.284 kb on + strand at 176.402 kb on + strand at 176.402 kb on + strand at 176.403 kb on - strand at 176.438 kb on + strand at 176.438 kb on + strand at 176.438 kb on + strand at 176.533 kb on + strand at 176.592 kb on + strand at 176.592 kb on + strand at 176.592 kb on + strand at 176.593 kb on - strand at 176.593 kb on - strand at 176.593 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 174,730 + Pf1N1B4_5434 0.79 -0.8 174,731 - Pf1N1B4_5434 0.79 -1.9 174,744 + Pf1N1B4_5434 0.80 +0.1 174,762 + Pf1N1B4_5434 0.81 -0.9 174,805 + Pf1N1B4_5434 0.85 -0.7 174,805 + Pf1N1B4_5434 0.85 +0.9 174,805 + Pf1N1B4_5434 0.85 +0.8 174,805 + Pf1N1B4_5434 0.85 -0.9 174,805 + Pf1N1B4_5434 0.85 +0.6 174,827 + Pf1N1B4_5434 0.86 +1.1 174,911 + -0.4 174,911 + +0.5 174,911 + +1.1 174,911 + -0.9 174,911 + +0.7 174,912 - +0.6 174,927 + +0.1 175,005 + -0.5 175,005 + +1.4 175,005 + -0.7 175,005 + -0.9 175,050 + -0.8 175,114 + +1.4 175,115 - +0.5 175,148 + +0.2 175,157 + -0.5 175,157 + -0.9 175,157 + +0.1 175,158 - +1.6 175,158 - +0.1 175,173 + -1.5 175,181 + -0.4 175,182 - +2.1 175,312 + +0.1 175,354 + +0.6 175,407 + +0.1 175,407 + +0.8 175,408 - +0.1 175,431 - -0.6 175,613 + -2.3 175,614 - -0.2 175,691 + +0.1 175,751 + Pf1N1B4_5435 0.14 +0.8 175,751 + Pf1N1B4_5435 0.14 +0.5 175,968 + -1.4 176,196 + -2.3 176,284 + -1.5 176,402 + -0.9 176,402 + +0.6 176,403 - +0.5 176,438 + +0.3 176,438 + +1.6 176,438 + +0.1 176,533 + +0.6 176,592 + +1.1 176,592 + -0.5 176,592 + -0.5 176,593 - +0.7 176,593 - +0.5 176,593 - -0.5
Or see this region's nucleotide sequence