Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5432 and Pf1N1B4_5433 are separated by 907 nucleotides Pf1N1B4_5433 and Pf1N1B4_5434 are separated by 868 nucleotides
Pf1N1B4_5432 - hypothetical protein, at 171,160 to 171,348
_5432
Pf1N1B4_5433 - hypothetical protein, at 172,256 to 172,825
_5433
Pf1N1B4_5434 - HIPA PROTEIN, at 173,694 to 175,007
_5434
Position (kb) 172
173 Strain fitness (log2 ratio) -2
-1
0
1
2 at 171.291 kb on - strand, within Pf1N1B4_5432 at 171.291 kb on - strand, within Pf1N1B4_5432 at 171.365 kb on - strand at 171.416 kb on + strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.561 kb on - strand at 171.585 kb on - strand at 171.667 kb on - strand at 171.705 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.733 kb on + strand at 171.733 kb on + strand at 171.733 kb on + strand at 171.734 kb on - strand at 171.756 kb on - strand at 171.761 kb on + strand at 171.762 kb on - strand at 171.762 kb on - strand at 171.849 kb on - strand at 171.855 kb on + strand at 171.887 kb on - strand at 171.892 kb on + strand at 171.954 kb on + strand at 171.954 kb on + strand at 171.965 kb on + strand at 171.966 kb on - strand at 172.073 kb on - strand at 172.108 kb on + strand at 172.243 kb on + strand at 172.243 kb on + strand at 172.243 kb on + strand at 172.244 kb on - strand at 172.255 kb on + strand at 172.255 kb on + strand at 172.316 kb on + strand, within Pf1N1B4_5433 at 172.494 kb on + strand, within Pf1N1B4_5433 at 172.495 kb on - strand at 172.515 kb on + strand, within Pf1N1B4_5433 at 172.515 kb on + strand, within Pf1N1B4_5433 at 172.753 kb on + strand, within Pf1N1B4_5433 at 172.753 kb on + strand, within Pf1N1B4_5433 at 172.890 kb on + strand at 173.004 kb on - strand at 173.004 kb on - strand at 173.110 kb on + strand at 173.196 kb on + strand at 173.196 kb on + strand at 173.196 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.274 kb on + strand at 173.275 kb on - strand at 173.275 kb on - strand at 173.275 kb on - strand at 173.276 kb on + strand at 173.276 kb on + strand at 173.276 kb on + strand at 173.277 kb on - strand at 173.285 kb on + strand at 173.285 kb on + strand at 173.286 kb on - strand at 173.389 kb on - strand at 173.584 kb on - strand at 173.584 kb on - strand at 173.617 kb on - strand at 173.617 kb on - strand at 173.664 kb on + strand at 173.665 kb on - strand at 173.665 kb on - strand at 173.665 kb on - strand at 173.686 kb on - strand at 173.699 kb on - strand at 173.716 kb on + strand at 173.716 kb on + strand at 173.716 kb on + strand at 173.716 kb on + strand at 173.717 kb on - strand at 173.717 kb on - strand at 173.717 kb on - strand at 173.717 kb on - strand at 173.717 kb on - strand at 173.784 kb on + strand at 173.784 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 171,291 - Pf1N1B4_5432 0.69 +1.6 171,291 - Pf1N1B4_5432 0.69 -1.3 171,365 - -0.5 171,416 + -0.4 171,417 - -1.5 171,417 - +0.5 171,417 - -1.5 171,417 - +0.1 171,417 - -0.3 171,561 - +0.3 171,585 - -1.1 171,667 - -0.9 171,705 + +0.9 171,729 + -0.5 171,729 + -1.4 171,729 + +0.4 171,729 + +0.8 171,730 - -0.5 171,730 - +1.2 171,730 - +0.5 171,730 - -0.5 171,733 + -0.9 171,733 + +0.1 171,733 + -0.4 171,734 - +0.1 171,756 - +0.5 171,761 + -0.4 171,762 - -1.5 171,762 - +0.9 171,849 - +0.1 171,855 + +0.1 171,887 - +0.1 171,892 + -0.9 171,954 + -0.2 171,954 + -0.9 171,965 + +1.2 171,966 - +0.6 172,073 - +0.1 172,108 + +1.1 172,243 + +2.1 172,243 + +0.6 172,243 + -0.3 172,244 - +0.1 172,255 + -2.3 172,255 + -1.0 172,316 + Pf1N1B4_5433 0.11 +1.4 172,494 + Pf1N1B4_5433 0.42 +0.9 172,495 - +0.1 172,515 + Pf1N1B4_5433 0.45 -0.2 172,515 + Pf1N1B4_5433 0.45 +0.2 172,753 + Pf1N1B4_5433 0.87 +0.1 172,753 + Pf1N1B4_5433 0.87 -0.2 172,890 + -0.4 173,004 - -0.9 173,004 - -0.1 173,110 + +0.1 173,196 + +0.5 173,196 + +1.5 173,196 + +0.6 173,274 + -1.3 173,274 + -0.4 173,274 + +1.1 173,274 + -0.5 173,274 + -0.9 173,274 + -1.2 173,274 + -1.2 173,274 + -0.9 173,274 + -0.5 173,274 + +0.4 173,274 + -0.9 173,274 + +0.4 173,275 - +1.5 173,275 - -0.8 173,275 - +1.3 173,276 + +0.3 173,276 + +1.6 173,276 + -1.5 173,277 - +0.1 173,285 + -1.5 173,285 + -0.1 173,286 - -0.2 173,389 - -0.6 173,584 - +0.6 173,584 - +0.4 173,617 - -0.5 173,617 - -1.5 173,664 + +0.1 173,665 - -0.4 173,665 - -0.9 173,665 - -0.5 173,686 - -1.5 173,699 - +1.1 173,716 + +0.4 173,716 + -1.9 173,716 + -0.4 173,716 + +0.1 173,717 - +0.6 173,717 - +0.1 173,717 - -0.5 173,717 - -1.3 173,717 - +0.1 173,784 + +0.4 173,784 + +0.2
Or see this region's nucleotide sequence