Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5350 and Pf1N1B4_5351 overlap by 24 nucleotides Pf1N1B4_5351 and Pf1N1B4_5352 overlap by 32 nucleotides Pf1N1B4_5352 and Pf1N1B4_5353 are separated by 516 nucleotides Pf1N1B4_5353 and Pf1N1B4_5354 are separated by 109 nucleotides Pf1N1B4_5354 and Pf1N1B4_5355 are separated by 17 nucleotides Pf1N1B4_5355 and Pf1N1B4_5356 are separated by 360 nucleotides
Pf1N1B4_5350 - hypothetical protein, at 90,270 to 91,034
_5350
Pf1N1B4_5351 - hypothetical protein, at 91,011 to 91,241
_5351
Pf1N1B4_5352 - hypothetical protein, at 91,210 to 91,479
_5352
Pf1N1B4_5353 - hypothetical protein, at 91,996 to 92,163
_5353
Pf1N1B4_5354 - hypothetical protein, at 92,273 to 92,416
_5354
Pf1N1B4_5355 - hypothetical protein, at 92,434 to 92,616
_5355
Pf1N1B4_5356 - hypothetical protein, at 92,977 to 93,222
_5356
Position (kb)
91
92
93 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 91.045 kb on + strand, within Pf1N1B4_5351 at 91.045 kb on + strand, within Pf1N1B4_5351 at 91.045 kb on + strand, within Pf1N1B4_5351 at 91.046 kb on - strand, within Pf1N1B4_5351 at 91.046 kb on - strand, within Pf1N1B4_5351 at 91.046 kb on - strand, within Pf1N1B4_5351 at 91.046 kb on - strand, within Pf1N1B4_5351 at 91.175 kb on + strand, within Pf1N1B4_5351 at 91.176 kb on - strand, within Pf1N1B4_5351 at 91.197 kb on + strand, within Pf1N1B4_5351 at 91.197 kb on + strand, within Pf1N1B4_5351 at 91.197 kb on + strand, within Pf1N1B4_5351 at 91.197 kb on + strand, within Pf1N1B4_5351 at 91.198 kb on - strand, within Pf1N1B4_5351 at 91.211 kb on + strand at 91.211 kb on + strand at 91.212 kb on - strand at 91.249 kb on + strand, within Pf1N1B4_5352 at 91.493 kb on + strand at 91.493 kb on + strand at 91.494 kb on - strand at 91.523 kb on + strand at 91.523 kb on + strand at 91.524 kb on - strand at 91.567 kb on + strand at 91.625 kb on + strand at 91.625 kb on + strand at 91.625 kb on + strand at 91.626 kb on - strand at 91.626 kb on - strand at 91.626 kb on - strand at 91.792 kb on + strand at 91.793 kb on - strand at 91.793 kb on - strand at 91.793 kb on - strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.872 kb on - strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.892 kb on - strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.908 kb on - strand at 91.908 kb on - strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.930 kb on - strand at 91.930 kb on - strand at 91.950 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.994 kb on - strand at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.093 kb on - strand, within Pf1N1B4_5353 at 92.093 kb on - strand, within Pf1N1B4_5353 at 92.161 kb on + strand at 92.165 kb on + strand at 92.165 kb on + strand at 92.186 kb on + strand at 92.302 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.339 kb on - strand, within Pf1N1B4_5354 at 92.349 kb on + strand, within Pf1N1B4_5354 at 92.349 kb on + strand, within Pf1N1B4_5354 at 92.385 kb on + strand, within Pf1N1B4_5354 at 92.443 kb on + strand at 92.443 kb on + strand at 92.496 kb on + strand, within Pf1N1B4_5355 at 92.496 kb on + strand at 92.497 kb on - strand, within Pf1N1B4_5355 at 92.500 kb on + strand, within Pf1N1B4_5355 at 92.500 kb on + strand, within Pf1N1B4_5355 at 92.501 kb on - strand, within Pf1N1B4_5355 at 92.501 kb on - strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.626 kb on + strand at 92.626 kb on + strand at 92.658 kb on + strand at 92.658 kb on + strand at 92.677 kb on + strand at 92.677 kb on + strand at 92.677 kb on + strand at 92.678 kb on - strand at 92.678 kb on - strand at 92.871 kb on + strand at 92.871 kb on + strand at 92.872 kb on - strand at 92.872 kb on - strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.938 kb on - strand at 92.938 kb on - strand at 92.938 kb on - strand at 92.939 kb on + strand at 92.939 kb on + strand at 92.939 kb on + strand at 92.955 kb on - strand at 92.955 kb on - strand at 92.955 kb on - strand at 92.966 kb on - strand at 92.995 kb on + strand at 92.996 kb on - strand at 92.996 kb on - strand at 93.001 kb on + strand at 93.001 kb on + strand at 93.036 kb on + strand, within Pf1N1B4_5356 at 93.114 kb on + strand, within Pf1N1B4_5356 at 93.147 kb on + strand, within Pf1N1B4_5356 at 93.147 kb on + strand, within Pf1N1B4_5356
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 91,045 + Pf1N1B4_5351 0.15 +0.8 91,045 + Pf1N1B4_5351 0.15 -1.5 91,045 + Pf1N1B4_5351 0.15 -0.5 91,046 - Pf1N1B4_5351 0.15 -0.4 91,046 - Pf1N1B4_5351 0.15 +1.1 91,046 - Pf1N1B4_5351 0.15 -0.9 91,046 - Pf1N1B4_5351 0.15 -0.5 91,175 + Pf1N1B4_5351 0.71 -0.1 91,176 - Pf1N1B4_5351 0.71 +0.2 91,197 + Pf1N1B4_5351 0.81 -0.4 91,197 + Pf1N1B4_5351 0.81 +1.1 91,197 + Pf1N1B4_5351 0.81 +1.9 91,197 + Pf1N1B4_5351 0.81 +0.1 91,198 - Pf1N1B4_5351 0.81 +0.5 91,211 + -0.5 91,211 + -1.5 91,212 - -0.1 91,249 + Pf1N1B4_5352 0.14 +0.4 91,493 + +0.1 91,493 + -2.3 91,494 - +0.6 91,523 + -2.3 91,523 + +0.4 91,524 - -1.2 91,567 + +0.1 91,625 + -0.7 91,625 + +0.5 91,625 + +0.4 91,626 - +1.1 91,626 - +0.1 91,626 - +1.6 91,792 + -1.5 91,793 - -0.4 91,793 - +1.1 91,793 - -0.9 91,794 + -1.5 91,794 + -0.1 91,794 + +0.8 91,794 + +0.1 91,794 + -0.2 91,794 + +0.3 91,871 + +1.5 91,871 + +0.6 91,871 + +0.3 91,871 + -0.2 91,871 + -0.1 91,871 + -0.2 91,871 + +0.6 91,871 + -0.7 91,871 + +0.5 91,872 - +2.1 91,891 + -0.7 91,891 + +0.1 91,891 + +1.4 91,891 + -0.6 91,891 + +0.1 91,891 + +0.8 91,892 - +0.2 91,907 + -0.5 91,907 + +0.8 91,907 + +0.3 91,907 + -0.5 91,907 + -0.5 91,908 - -0.7 91,908 - -0.1 91,917 + -0.1 91,917 + +0.1 91,917 + -1.3 91,917 + +0.1 91,917 + +0.5 91,917 + +0.1 91,917 + -0.1 91,929 + +0.1 91,929 + -1.5 91,929 + -0.4 91,929 + -1.3 91,929 + +0.4 91,929 + +0.5 91,929 + +1.4 91,929 + -0.2 91,929 + +0.9 91,930 - -0.9 91,930 - -1.9 91,950 + -0.1 91,993 + -0.8 91,993 + -0.4 91,993 + +1.6 91,993 + -0.3 91,994 - -0.9 92,092 + Pf1N1B4_5353 0.57 +1.4 92,092 + Pf1N1B4_5353 0.57 +1.5 92,092 + Pf1N1B4_5353 0.57 +0.5 92,092 + Pf1N1B4_5353 0.57 -1.5 92,092 + Pf1N1B4_5353 0.57 +0.8 92,092 + Pf1N1B4_5353 0.57 -2.3 92,092 + Pf1N1B4_5353 0.57 +0.1 92,093 - Pf1N1B4_5353 0.58 -0.3 92,093 - Pf1N1B4_5353 0.58 +1.6 92,161 + +0.9 92,165 + +0.1 92,165 + +0.2 92,186 + +0.2 92,302 + Pf1N1B4_5354 0.20 -0.4 92,338 + Pf1N1B4_5354 0.45 -0.1 92,338 + Pf1N1B4_5354 0.45 +1.1 92,338 + Pf1N1B4_5354 0.45 +0.6 92,339 - Pf1N1B4_5354 0.46 +0.1 92,349 + Pf1N1B4_5354 0.53 -0.4 92,349 + Pf1N1B4_5354 0.53 -2.9 92,385 + Pf1N1B4_5354 0.78 -0.8 92,443 + -0.8 92,443 + +0.1 92,496 + Pf1N1B4_5355 0.34 +3.1 92,496 + -0.9 92,497 - Pf1N1B4_5355 0.34 -0.5 92,500 + Pf1N1B4_5355 0.36 +0.6 92,500 + Pf1N1B4_5355 0.36 -0.2 92,501 - Pf1N1B4_5355 0.37 -0.3 92,501 - Pf1N1B4_5355 0.37 -1.5 92,539 + Pf1N1B4_5355 0.57 +0.4 92,539 + Pf1N1B4_5355 0.57 -0.6 92,539 + Pf1N1B4_5355 0.57 +0.1 92,539 + Pf1N1B4_5355 0.57 +0.1 92,539 + Pf1N1B4_5355 0.57 -0.6 92,539 + Pf1N1B4_5355 0.57 -1.2 92,539 + Pf1N1B4_5355 0.57 -0.5 92,539 + Pf1N1B4_5355 0.57 +0.7 92,539 + Pf1N1B4_5355 0.57 +0.1 92,539 + Pf1N1B4_5355 0.57 +1.0 92,539 + Pf1N1B4_5355 0.57 +0.1 92,541 + Pf1N1B4_5355 0.58 -1.5 92,541 + Pf1N1B4_5355 0.58 +1.2 92,541 + Pf1N1B4_5355 0.58 -0.6 92,626 + -0.3 92,626 + +0.4 92,658 + +0.1 92,658 + -1.4 92,677 + -1.4 92,677 + -1.5 92,677 + -0.5 92,678 - +0.2 92,678 - -0.5 92,871 + +0.9 92,871 + +0.1 92,872 - +0.6 92,872 - +0.1 92,934 + -0.2 92,934 + +0.1 92,934 + -0.6 92,934 + +0.1 92,934 + +0.1 92,935 - +0.4 92,935 - -1.0 92,935 - -0.5 92,935 - +0.5 92,935 - -0.5 92,935 - +0.1 92,935 - +0.1 92,935 - -1.5 92,935 - +2.4 92,937 + +1.1 92,937 + +1.1 92,937 + -0.3 92,937 + +0.4 92,937 + +0.1 92,937 + +0.6 92,937 + -1.5 92,937 + +0.5 92,937 + +2.1 92,938 - +1.1 92,938 - -1.5 92,938 - -0.6 92,939 + +0.1 92,939 + +1.6 92,939 + +0.1 92,955 - -1.9 92,955 - +0.1 92,955 - -0.5 92,966 - +0.4 92,995 + +0.6 92,996 - -1.9 92,996 - +1.1 93,001 + +0.5 93,001 + +1.1 93,036 + Pf1N1B4_5356 0.24 +0.4 93,114 + Pf1N1B4_5356 0.56 +0.1 93,147 + Pf1N1B4_5356 0.69 -0.4 93,147 + Pf1N1B4_5356 0.69 -1.8
Or see this region's nucleotide sequence