Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5281 and Pf1N1B4_5282 are separated by 212 nucleotides Pf1N1B4_5282 and Pf1N1B4_5283 are separated by 47 nucleotides Pf1N1B4_5283 and Pf1N1B4_5284 are separated by 327 nucleotides
Pf1N1B4_5281 - hypothetical protein, at 35,503 to 36,150
_5281
Pf1N1B4_5282 - Phage protein, at 36,363 to 36,533
_5282
Pf1N1B4_5283 - hypothetical protein, at 36,581 to 36,943
_5283
Pf1N1B4_5284 - hypothetical protein, at 37,271 to 37,441
_5284
Position (kb) 36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 35.609 kb on + strand, within Pf1N1B4_5281 at 35.609 kb on + strand at 35.610 kb on - strand, within Pf1N1B4_5281 at 35.610 kb on - strand, within Pf1N1B4_5281 at 35.610 kb on - strand, within Pf1N1B4_5281 at 35.610 kb on - strand, within Pf1N1B4_5281 at 35.632 kb on + strand, within Pf1N1B4_5281 at 35.632 kb on + strand, within Pf1N1B4_5281 at 35.633 kb on - strand, within Pf1N1B4_5281 at 35.633 kb on - strand, within Pf1N1B4_5281 at 35.633 kb on - strand, within Pf1N1B4_5281 at 35.633 kb on - strand, within Pf1N1B4_5281 at 35.700 kb on + strand, within Pf1N1B4_5281 at 35.700 kb on + strand, within Pf1N1B4_5281 at 35.701 kb on - strand at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.701 kb on - strand, within Pf1N1B4_5281 at 35.715 kb on + strand, within Pf1N1B4_5281 at 35.715 kb on + strand, within Pf1N1B4_5281 at 35.715 kb on + strand at 35.716 kb on - strand, within Pf1N1B4_5281 at 35.716 kb on - strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.747 kb on + strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.748 kb on - strand, within Pf1N1B4_5281 at 35.793 kb on - strand, within Pf1N1B4_5281 at 35.857 kb on + strand, within Pf1N1B4_5281 at 35.858 kb on - strand, within Pf1N1B4_5281 at 35.858 kb on - strand, within Pf1N1B4_5281 at 35.897 kb on + strand, within Pf1N1B4_5281 at 35.897 kb on + strand, within Pf1N1B4_5281 at 35.897 kb on + strand, within Pf1N1B4_5281 at 35.898 kb on - strand, within Pf1N1B4_5281 at 35.898 kb on - strand, within Pf1N1B4_5281 at 36.150 kb on + strand at 36.151 kb on - strand at 36.151 kb on - strand at 36.171 kb on + strand at 36.171 kb on + strand at 36.223 kb on + strand at 36.223 kb on + strand at 36.223 kb on + strand at 36.223 kb on + strand at 36.223 kb on + strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.224 kb on - strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.341 kb on + strand at 36.342 kb on - strand at 36.342 kb on - strand at 36.342 kb on - strand at 36.342 kb on - strand at 36.389 kb on - strand, within Pf1N1B4_5282 at 36.417 kb on + strand, within Pf1N1B4_5282 at 36.417 kb on + strand at 36.417 kb on + strand, within Pf1N1B4_5282 at 36.417 kb on + strand, within Pf1N1B4_5282 at 36.446 kb on + strand, within Pf1N1B4_5282 at 36.446 kb on + strand, within Pf1N1B4_5282 at 36.446 kb on + strand, within Pf1N1B4_5282 at 36.447 kb on - strand, within Pf1N1B4_5282 at 36.447 kb on - strand, within Pf1N1B4_5282 at 36.531 kb on + strand at 36.633 kb on - strand at 36.635 kb on + strand, within Pf1N1B4_5283 at 36.635 kb on + strand, within Pf1N1B4_5283 at 36.635 kb on + strand, within Pf1N1B4_5283 at 36.635 kb on + strand, within Pf1N1B4_5283 at 36.890 kb on - strand, within Pf1N1B4_5283 at 36.941 kb on + strand at 36.941 kb on + strand at 36.942 kb on - strand at 36.942 kb on - strand at 36.960 kb on + strand at 36.961 kb on - strand at 37.001 kb on + strand at 37.001 kb on + strand at 37.001 kb on + strand at 37.052 kb on - strand at 37.079 kb on + strand at 37.080 kb on - strand at 37.080 kb on - strand at 37.170 kb on + strand at 37.171 kb on - strand at 37.198 kb on + strand at 37.199 kb on - strand at 37.201 kb on + strand at 37.201 kb on + strand at 37.201 kb on + strand at 37.202 kb on - strand at 37.202 kb on - strand at 37.202 kb on - strand at 37.202 kb on - strand at 37.248 kb on - strand at 37.295 kb on + strand, within Pf1N1B4_5284 at 37.316 kb on + strand, within Pf1N1B4_5284 at 37.356 kb on + strand, within Pf1N1B4_5284 at 37.356 kb on + strand, within Pf1N1B4_5284 at 37.357 kb on - strand, within Pf1N1B4_5284 at 37.368 kb on + strand, within Pf1N1B4_5284 at 37.369 kb on - strand at 37.369 kb on - strand, within Pf1N1B4_5284 at 37.476 kb on - strand at 37.484 kb on - strand at 37.519 kb on - strand at 37.535 kb on + strand at 37.536 kb on - strand at 37.552 kb on + strand at 37.599 kb on + strand at 37.599 kb on + strand at 37.599 kb on + strand at 37.600 kb on - strand at 37.600 kb on - strand at 37.686 kb on + strand at 37.687 kb on - strand at 37.714 kb on - strand at 37.765 kb on + strand at 37.766 kb on - strand at 37.766 kb on - strand at 37.796 kb on + strand at 37.796 kb on + strand at 37.797 kb on - strand at 37.817 kb on + strand at 37.817 kb on + strand at 37.818 kb on - strand at 37.821 kb on + strand at 37.822 kb on - strand at 37.822 kb on - strand at 37.837 kb on + strand at 37.837 kb on + strand at 37.837 kb on + strand at 37.837 kb on + strand at 37.838 kb on - strand at 37.878 kb on + strand at 37.878 kb on + strand at 37.878 kb on + strand at 37.878 kb on + strand at 37.878 kb on + strand at 37.879 kb on - strand at 37.883 kb on + strand at 37.895 kb on + strand at 37.896 kb on - strand at 37.901 kb on + strand at 37.901 kb on + strand at 37.902 kb on - strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.903 kb on + strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.904 kb on - strand at 37.938 kb on + strand at 37.938 kb on + strand at 37.938 kb on + strand at 37.938 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 35,609 + Pf1N1B4_5281 0.16 -0.9 35,609 + -1.5 35,610 - Pf1N1B4_5281 0.17 -2.9 35,610 - Pf1N1B4_5281 0.17 +0.4 35,610 - Pf1N1B4_5281 0.17 -0.2 35,610 - Pf1N1B4_5281 0.17 -0.4 35,632 + Pf1N1B4_5281 0.20 +0.6 35,632 + Pf1N1B4_5281 0.20 +0.6 35,633 - Pf1N1B4_5281 0.20 +1.1 35,633 - Pf1N1B4_5281 0.20 +1.1 35,633 - Pf1N1B4_5281 0.20 -0.6 35,633 - Pf1N1B4_5281 0.20 -0.4 35,700 + Pf1N1B4_5281 0.30 +0.8 35,700 + Pf1N1B4_5281 0.30 -0.5 35,701 - +0.1 35,701 - Pf1N1B4_5281 0.31 +0.1 35,701 - Pf1N1B4_5281 0.31 +0.1 35,701 - Pf1N1B4_5281 0.31 -0.9 35,701 - Pf1N1B4_5281 0.31 +0.1 35,701 - Pf1N1B4_5281 0.31 -0.9 35,715 + Pf1N1B4_5281 0.33 +1.1 35,715 + Pf1N1B4_5281 0.33 +0.1 35,715 + -1.5 35,716 - Pf1N1B4_5281 0.33 -1.5 35,716 - Pf1N1B4_5281 0.33 -0.2 35,747 + Pf1N1B4_5281 0.38 -1.3 35,747 + Pf1N1B4_5281 0.38 -0.2 35,747 + Pf1N1B4_5281 0.38 +0.1 35,747 + Pf1N1B4_5281 0.38 -1.3 35,747 + Pf1N1B4_5281 0.38 -0.9 35,747 + Pf1N1B4_5281 0.38 -1.8 35,748 - Pf1N1B4_5281 0.38 -0.5 35,748 - Pf1N1B4_5281 0.38 -0.9 35,748 - Pf1N1B4_5281 0.38 -1.5 35,748 - +0.1 35,748 - Pf1N1B4_5281 0.38 -0.2 35,748 - Pf1N1B4_5281 0.38 -0.9 35,748 - Pf1N1B4_5281 0.38 -0.5 35,748 - Pf1N1B4_5281 0.38 -0.3 35,748 - Pf1N1B4_5281 0.38 +1.6 35,748 - Pf1N1B4_5281 0.38 +0.3 35,793 - Pf1N1B4_5281 0.45 -0.9 35,857 + Pf1N1B4_5281 0.55 -1.5 35,858 - Pf1N1B4_5281 0.55 +0.6 35,858 - Pf1N1B4_5281 0.55 +0.1 35,897 + Pf1N1B4_5281 0.61 -0.9 35,897 + Pf1N1B4_5281 0.61 -0.9 35,897 + Pf1N1B4_5281 0.61 -1.5 35,898 - Pf1N1B4_5281 0.61 +0.1 35,898 - Pf1N1B4_5281 0.61 +0.8 36,150 + -0.5 36,151 - -2.9 36,151 - -1.5 36,171 + +0.6 36,171 + +1.4 36,223 + -1.2 36,223 + -0.9 36,223 + -0.5 36,223 + -0.9 36,223 + +0.4 36,224 - +0.1 36,224 - -0.6 36,224 - -0.5 36,224 - -1.5 36,224 - -0.5 36,224 - -0.7 36,224 - +1.1 36,224 - +1.1 36,224 - -0.5 36,341 + -0.2 36,341 + -0.7 36,341 + -1.3 36,341 + -1.2 36,341 + +0.3 36,341 + -1.9 36,341 + +0.1 36,342 - -0.9 36,342 - -0.1 36,342 - -0.2 36,342 - +1.1 36,389 - Pf1N1B4_5282 0.15 +1.1 36,417 + Pf1N1B4_5282 0.32 -0.4 36,417 + +0.1 36,417 + Pf1N1B4_5282 0.32 -0.9 36,417 + Pf1N1B4_5282 0.32 -0.7 36,446 + Pf1N1B4_5282 0.49 -0.2 36,446 + Pf1N1B4_5282 0.49 +0.3 36,446 + Pf1N1B4_5282 0.49 +1.6 36,447 - Pf1N1B4_5282 0.49 +2.4 36,447 - Pf1N1B4_5282 0.49 +1.1 36,531 + +0.4 36,633 - +0.1 36,635 + Pf1N1B4_5283 0.15 +0.1 36,635 + Pf1N1B4_5283 0.15 +1.6 36,635 + Pf1N1B4_5283 0.15 +0.1 36,635 + Pf1N1B4_5283 0.15 +0.1 36,890 - Pf1N1B4_5283 0.85 +1.1 36,941 + +1.6 36,941 + +0.2 36,942 - +0.4 36,942 - +0.6 36,960 + -0.9 36,961 - -1.1 37,001 + -1.8 37,001 + -1.5 37,001 + +0.1 37,052 - +0.6 37,079 + -0.9 37,080 - +0.1 37,080 - -0.2 37,170 + -1.2 37,171 - -1.9 37,198 + +0.5 37,199 - -1.5 37,201 + +0.1 37,201 + +0.6 37,201 + -0.7 37,202 - -0.9 37,202 - +0.1 37,202 - -0.3 37,202 - +0.1 37,248 - +0.6 37,295 + Pf1N1B4_5284 0.14 -0.4 37,316 + Pf1N1B4_5284 0.26 -0.5 37,356 + Pf1N1B4_5284 0.50 +0.6 37,356 + Pf1N1B4_5284 0.50 -0.4 37,357 - Pf1N1B4_5284 0.50 +1.1 37,368 + Pf1N1B4_5284 0.57 +0.1 37,369 - +0.1 37,369 - Pf1N1B4_5284 0.57 +0.5 37,476 - +0.1 37,484 - +0.1 37,519 - +0.3 37,535 + +2.1 37,536 - +0.3 37,552 + -0.1 37,599 + -0.1 37,599 + +0.1 37,599 + -0.9 37,600 - -0.9 37,600 - +1.1 37,686 + +0.1 37,687 - +0.6 37,714 - -1.3 37,765 + -1.2 37,766 - +0.1 37,766 - -0.3 37,796 + -1.1 37,796 + -1.6 37,797 - +1.1 37,817 + -1.3 37,817 + -1.5 37,818 - -1.3 37,821 + -0.9 37,822 - -0.4 37,822 - +1.6 37,837 + +0.1 37,837 + +1.4 37,837 + +1.1 37,837 + -1.5 37,838 - -0.7 37,878 + +1.3 37,878 + +0.5 37,878 + -2.5 37,878 + +1.1 37,878 + -0.5 37,879 - +0.6 37,883 + -0.3 37,895 + +0.1 37,896 - +0.5 37,901 + -0.5 37,901 + -1.8 37,902 - +0.6 37,903 + +0.1 37,903 + +0.5 37,903 + +0.1 37,903 + +0.9 37,903 + +0.6 37,903 + +2.4 37,903 + +0.1 37,903 + +1.1 37,903 + -0.8 37,903 + +0.5 37,903 + +0.7 37,903 + +0.6 37,903 + -0.8 37,903 + +0.6 37,903 + +0.6 37,903 + +0.4 37,904 - +1.4 37,904 - +0.5 37,904 - +0.8 37,904 - -0.3 37,904 - +1.9 37,904 - +2.4 37,938 + +0.1 37,938 + +0.8 37,938 + -1.3 37,938 + -1.5
Or see this region's nucleotide sequence