Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5271 and Pf1N1B4_5272 are separated by 255 nucleotides Pf1N1B4_5272 and Pf1N1B4_5273 are separated by 123 nucleotides Pf1N1B4_5273 and Pf1N1B4_5274 are separated by 176 nucleotides Pf1N1B4_5274 and Pf1N1B4_5275 are separated by 424 nucleotides
Pf1N1B4_5271 - hypothetical protein, at 28,584 to 28,769
_5271
Pf1N1B4_5272 - hypothetical protein, at 29,025 to 29,201
_5272
Pf1N1B4_5273 - hypothetical protein, at 29,325 to 29,489
_5273
Pf1N1B4_5274 - hypothetical protein, at 29,666 to 29,947
_5274
Pf1N1B4_5275 - hypothetical protein, at 30,372 to 30,725
_5275
Position (kb) 29
30 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 28.732 kb on - strand, within Pf1N1B4_5271 at 28.836 kb on + strand at 28.837 kb on - strand at 28.945 kb on - strand at 28.948 kb on - strand at 28.948 kb on - strand at 28.971 kb on - strand at 28.971 kb on - strand at 28.971 kb on - strand at 28.989 kb on + strand at 29.212 kb on + strand at 29.594 kb on + strand at 29.594 kb on + strand at 29.595 kb on - strand at 29.595 kb on - strand at 29.595 kb on - strand at 29.595 kb on - strand at 29.595 kb on - strand at 29.656 kb on + strand at 29.656 kb on + strand at 29.708 kb on + strand, within Pf1N1B4_5274 at 29.709 kb on - strand, within Pf1N1B4_5274 at 29.709 kb on - strand, within Pf1N1B4_5274 at 29.780 kb on + strand, within Pf1N1B4_5274 at 29.780 kb on + strand, within Pf1N1B4_5274 at 29.780 kb on + strand, within Pf1N1B4_5274 at 29.780 kb on + strand, within Pf1N1B4_5274 at 29.781 kb on - strand, within Pf1N1B4_5274 at 29.781 kb on - strand, within Pf1N1B4_5274 at 29.872 kb on + strand, within Pf1N1B4_5274 at 29.872 kb on + strand, within Pf1N1B4_5274 at 29.912 kb on + strand, within Pf1N1B4_5274 at 29.912 kb on + strand, within Pf1N1B4_5274 at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.924 kb on + strand at 29.925 kb on - strand at 29.925 kb on - strand at 29.925 kb on - strand at 29.945 kb on + strand at 29.945 kb on + strand at 29.946 kb on - strand at 29.946 kb on - strand at 29.985 kb on + strand at 29.985 kb on + strand at 29.985 kb on + strand at 30.143 kb on + strand at 30.143 kb on + strand at 30.143 kb on + strand at 30.143 kb on + strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.144 kb on - strand at 30.212 kb on - strand at 30.212 kb on - strand at 30.278 kb on + strand at 30.297 kb on + strand at 30.383 kb on + strand at 30.384 kb on - strand at 30.384 kb on - strand at 30.564 kb on + strand, within Pf1N1B4_5275 at 30.565 kb on - strand, within Pf1N1B4_5275 at 30.565 kb on - strand, within Pf1N1B4_5275 at 30.600 kb on + strand, within Pf1N1B4_5275 at 30.600 kb on + strand at 30.600 kb on + strand, within Pf1N1B4_5275 at 30.723 kb on + strand at 30.724 kb on - strand at 30.780 kb on + strand at 30.805 kb on + strand at 30.904 kb on + strand at 30.919 kb on + strand at 30.919 kb on + strand at 30.945 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 28,732 - Pf1N1B4_5271 0.80 +0.2 28,836 + -0.8 28,837 - +1.6 28,945 - -0.5 28,948 - +0.1 28,948 - +0.5 28,971 - -0.9 28,971 - +0.1 28,971 - -0.9 28,989 + -1.3 29,212 + -1.5 29,594 + +0.1 29,594 + -0.9 29,595 - -1.3 29,595 - -1.9 29,595 - +0.8 29,595 - +0.4 29,595 - -0.9 29,656 + -0.8 29,656 + +0.6 29,708 + Pf1N1B4_5274 0.15 -1.3 29,709 - Pf1N1B4_5274 0.15 +0.1 29,709 - Pf1N1B4_5274 0.15 -0.9 29,780 + Pf1N1B4_5274 0.40 -1.8 29,780 + Pf1N1B4_5274 0.40 -0.4 29,780 + Pf1N1B4_5274 0.40 -1.6 29,780 + Pf1N1B4_5274 0.40 -0.4 29,781 - Pf1N1B4_5274 0.41 -0.5 29,781 - Pf1N1B4_5274 0.41 -0.9 29,872 + Pf1N1B4_5274 0.73 -1.9 29,872 + Pf1N1B4_5274 0.73 -0.7 29,912 + Pf1N1B4_5274 0.87 -0.9 29,912 + Pf1N1B4_5274 0.87 -2.3 29,924 + -0.9 29,924 + +0.5 29,924 + -2.8 29,924 + -0.3 29,924 + +0.6 29,924 + +1.1 29,924 + +0.6 29,924 + -0.1 29,924 + -1.4 29,925 - +0.1 29,925 - -0.9 29,925 - +0.1 29,945 + +1.1 29,945 + +2.1 29,946 - +0.1 29,946 - -1.1 29,985 + -0.4 29,985 + +0.8 29,985 + +0.1 30,143 + -0.9 30,143 + -1.5 30,143 + -1.9 30,143 + -1.9 30,144 - +1.6 30,144 - -0.9 30,144 - -0.5 30,144 - -0.4 30,144 - -0.3 30,144 - -2.3 30,212 - +0.3 30,212 - +1.1 30,278 + -1.5 30,297 + -1.5 30,383 + -0.9 30,384 - -0.7 30,384 - -1.5 30,564 + Pf1N1B4_5275 0.54 +1.1 30,565 - Pf1N1B4_5275 0.55 -0.1 30,565 - Pf1N1B4_5275 0.55 +0.1 30,600 + Pf1N1B4_5275 0.64 -0.5 30,600 + +0.1 30,600 + Pf1N1B4_5275 0.64 +0.4 30,723 + +1.1 30,724 - -0.5 30,780 + -0.8 30,805 + +1.8 30,904 + -1.2 30,919 + +0.6 30,919 + +0.1 30,945 + -1.9
Or see this region's nucleotide sequence