Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3456 and Pf1N1B4_3457 overlap by 26 nucleotides Pf1N1B4_3457 and Pf1N1B4_3458 are separated by 23 nucleotides Pf1N1B4_3458 and Pf1N1B4_5224 are separated by 292 nucleotides Pf1N1B4_5224 and Pf1N1B4_3459 are separated by 86 nucleotides
Pf1N1B4_3456 - ATP-binding protein PhnN; Guanylate kinase (EC 2.7.4.8), at 3,853,874 to 3,854,422
_3456
Pf1N1B4_3457 - hypothetical protein, at 3,854,397 to 3,854,576
_3457
Pf1N1B4_3458 - hypothetical protein, at 3,854,600 to 3,854,833
_3458
Pf1N1B4_5224 - tRNA-Arg-CCT, at 3,855,126 to 3,855,199
_5224
Pf1N1B4_3459 - T1SS secreted agglutinin RTX, at 3,855,286 to 3,862,629
_3459
Position (kb)
3855
3856 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3854.188 kb on - strand, within Pf1N1B4_3456 at 3854.188 kb on - strand at 3854.191 kb on - strand, within Pf1N1B4_3456 at 3854.199 kb on + strand, within Pf1N1B4_3456 at 3854.239 kb on - strand, within Pf1N1B4_3456 at 3854.323 kb on + strand, within Pf1N1B4_3456 at 3854.323 kb on + strand, within Pf1N1B4_3456 at 3854.359 kb on + strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.364 kb on + strand, within Pf1N1B4_3456 at 3854.365 kb on - strand, within Pf1N1B4_3456 at 3854.570 kb on + strand at 3854.571 kb on - strand at 3854.571 kb on - strand at 3854.642 kb on - strand, within Pf1N1B4_3458 at 3854.642 kb on - strand at 3854.724 kb on + strand, within Pf1N1B4_3458 at 3854.725 kb on - strand, within Pf1N1B4_3458 at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.030 kb on - strand at 3855.035 kb on + strand at 3855.036 kb on - strand at 3855.036 kb on - strand at 3855.133 kb on + strand at 3855.146 kb on + strand, within Pf1N1B4_5224 at 3855.146 kb on + strand, within Pf1N1B4_5224 at 3855.147 kb on - strand, within Pf1N1B4_5224 at 3855.162 kb on + strand, within Pf1N1B4_5224 at 3855.162 kb on + strand, within Pf1N1B4_5224 at 3855.163 kb on - strand, within Pf1N1B4_5224 at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.206 kb on - strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.387 kb on + strand at 3855.539 kb on + strand at 3855.540 kb on - strand at 3855.540 kb on - strand at 3855.578 kb on + strand at 3855.579 kb on - strand at 3855.579 kb on - strand at 3855.579 kb on - strand at 3855.579 kb on - strand at 3855.588 kb on + strand at 3855.589 kb on - strand at 3855.589 kb on - strand at 3855.589 kb on - strand at 3855.667 kb on + strand at 3855.667 kb on + strand at 3855.667 kb on + strand at 3855.667 kb on + strand at 3855.668 kb on - strand at 3855.668 kb on - strand at 3855.716 kb on + strand at 3855.717 kb on - strand at 3855.717 kb on - strand at 3855.717 kb on - strand at 3855.717 kb on - strand at 3855.770 kb on + strand at 3855.770 kb on + strand at 3855.771 kb on - strand at 3855.854 kb on + strand at 3855.855 kb on - strand at 3856.108 kb on + strand, within Pf1N1B4_3459 at 3856.109 kb on - strand, within Pf1N1B4_3459
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,854,188 - Pf1N1B4_3456 0.57 +1.0 3,854,188 - -1.7 3,854,191 - Pf1N1B4_3456 0.58 +0.4 3,854,199 + Pf1N1B4_3456 0.59 -1.1 3,854,239 - Pf1N1B4_3456 0.66 +0.3 3,854,323 + Pf1N1B4_3456 0.82 -1.0 3,854,323 + Pf1N1B4_3456 0.82 -0.1 3,854,359 + Pf1N1B4_3456 0.88 +1.3 3,854,360 - Pf1N1B4_3456 0.89 +1.3 3,854,360 - Pf1N1B4_3456 0.89 +0.9 3,854,360 - Pf1N1B4_3456 0.89 +0.5 3,854,360 - Pf1N1B4_3456 0.89 -0.1 3,854,364 + Pf1N1B4_3456 0.89 -0.6 3,854,365 - Pf1N1B4_3456 0.89 -0.1 3,854,570 + -0.1 3,854,571 - +0.1 3,854,571 - +0.9 3,854,642 - Pf1N1B4_3458 0.18 -0.1 3,854,642 - -1.1 3,854,724 + Pf1N1B4_3458 0.53 +0.0 3,854,725 - Pf1N1B4_3458 0.53 -0.1 3,855,029 + -0.6 3,855,029 + +2.2 3,855,029 + -0.1 3,855,029 + +0.3 3,855,030 - -0.1 3,855,035 + -0.1 3,855,036 - -0.1 3,855,036 - -0.1 3,855,133 + -1.4 3,855,146 + Pf1N1B4_5224 0.27 -1.1 3,855,146 + Pf1N1B4_5224 0.27 -1.6 3,855,147 - Pf1N1B4_5224 0.28 -2.1 3,855,162 + Pf1N1B4_5224 0.49 -0.9 3,855,162 + Pf1N1B4_5224 0.49 -1.9 3,855,163 - Pf1N1B4_5224 0.50 -2.7 3,855,205 + -2.1 3,855,205 + +1.7 3,855,205 + -1.1 3,855,205 + -0.7 3,855,205 + -0.1 3,855,205 + -0.7 3,855,206 - +0.7 3,855,300 + -0.3 3,855,300 + +1.6 3,855,300 + -0.4 3,855,300 + -0.9 3,855,300 + -0.6 3,855,301 - -0.5 3,855,301 - -0.1 3,855,301 - +0.5 3,855,301 - -0.1 3,855,387 + +0.6 3,855,539 + +1.1 3,855,540 - +0.2 3,855,540 - +1.9 3,855,578 + -0.1 3,855,579 - +0.3 3,855,579 - -0.1 3,855,579 - -0.9 3,855,579 - -0.1 3,855,588 + -0.5 3,855,589 - -0.7 3,855,589 - -1.7 3,855,589 - -2.1 3,855,667 + -0.6 3,855,667 + +0.9 3,855,667 + -0.1 3,855,667 + -0.2 3,855,668 - -0.1 3,855,668 - +0.9 3,855,716 + -0.7 3,855,717 - +0.9 3,855,717 - -0.1 3,855,717 - -1.4 3,855,717 - -1.1 3,855,770 + +0.7 3,855,770 + -0.1 3,855,771 - -1.5 3,855,854 + +0.4 3,855,855 - -0.1 3,856,108 + Pf1N1B4_3459 0.11 +0.6 3,856,109 - Pf1N1B4_3459 0.11 -0.7
Or see this region's nucleotide sequence