Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1814 and Pf1N1B4_5187 are separated by 63 nucleotides Pf1N1B4_5187 and Pf1N1B4_5188 are separated by 156 nucleotides
Pf1N1B4_1814 - Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2), at 2,085,058 to 2,086,254
_1814
Pf1N1B4_5187 - 5S RNA, at 2,086,318 to 2,086,437
_5187
Pf1N1B4_5188 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 2,086,594 to 2,089,486
_5188
Position (kb)
2086
2087 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2085.382 kb on + strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.481 kb on + strand, within Pf1N1B4_1814 at 2085.482 kb on - strand, within Pf1N1B4_1814 at 2085.482 kb on - strand, within Pf1N1B4_1814 at 2085.482 kb on - strand, within Pf1N1B4_1814 at 2085.501 kb on + strand, within Pf1N1B4_1814 at 2085.501 kb on + strand, within Pf1N1B4_1814 at 2085.502 kb on - strand, within Pf1N1B4_1814 at 2085.834 kb on + strand, within Pf1N1B4_1814 at 2085.859 kb on + strand, within Pf1N1B4_1814 at 2085.859 kb on + strand, within Pf1N1B4_1814 at 2085.860 kb on - strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.922 kb on + strand, within Pf1N1B4_1814 at 2085.923 kb on - strand, within Pf1N1B4_1814 at 2085.923 kb on - strand, within Pf1N1B4_1814 at 2085.923 kb on - strand, within Pf1N1B4_1814 at 2085.943 kb on + strand, within Pf1N1B4_1814 at 2086.139 kb on + strand at 2086.139 kb on + strand at 2086.139 kb on + strand at 2086.139 kb on + strand at 2086.157 kb on + strand at 2086.157 kb on + strand at 2086.158 kb on - strand at 2086.158 kb on - strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.178 kb on - strand at 2086.178 kb on - strand at 2086.178 kb on - strand at 2086.178 kb on - strand at 2086.179 kb on + strand at 2086.212 kb on + strand at 2086.213 kb on - strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.225 kb on - strand at 2086.261 kb on + strand at 2086.261 kb on + strand at 2086.261 kb on + strand at 2086.261 kb on + strand at 2086.293 kb on + strand at 2086.294 kb on - strand at 2086.335 kb on - strand, within Pf1N1B4_5187 at 2086.335 kb on - strand, within Pf1N1B4_5187 at 2086.593 kb on - strand at 2086.595 kb on - strand at 2086.595 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,085,382 + Pf1N1B4_1814 0.27 -0.7 2,085,383 - Pf1N1B4_1814 0.27 -0.7 2,085,383 - Pf1N1B4_1814 0.27 -2.1 2,085,383 - Pf1N1B4_1814 0.27 -1.1 2,085,383 - Pf1N1B4_1814 0.27 +0.5 2,085,383 - Pf1N1B4_1814 0.27 +0.9 2,085,481 + Pf1N1B4_1814 0.35 -1.8 2,085,482 - Pf1N1B4_1814 0.35 +0.5 2,085,482 - Pf1N1B4_1814 0.35 -2.1 2,085,482 - Pf1N1B4_1814 0.35 +0.3 2,085,501 + Pf1N1B4_1814 0.37 +0.4 2,085,501 + Pf1N1B4_1814 0.37 +0.5 2,085,502 - Pf1N1B4_1814 0.37 -0.8 2,085,834 + Pf1N1B4_1814 0.65 +0.9 2,085,859 + Pf1N1B4_1814 0.67 -0.1 2,085,859 + Pf1N1B4_1814 0.67 -1.4 2,085,860 - Pf1N1B4_1814 0.67 +0.3 2,085,919 + Pf1N1B4_1814 0.72 -0.5 2,085,919 + Pf1N1B4_1814 0.72 +0.5 2,085,919 + Pf1N1B4_1814 0.72 -1.5 2,085,919 + Pf1N1B4_1814 0.72 -1.1 2,085,919 + Pf1N1B4_1814 0.72 -0.3 2,085,919 + Pf1N1B4_1814 0.72 +0.5 2,085,919 + Pf1N1B4_1814 0.72 -0.5 2,085,920 - Pf1N1B4_1814 0.72 -0.1 2,085,920 - Pf1N1B4_1814 0.72 +3.1 2,085,920 - Pf1N1B4_1814 0.72 +2.1 2,085,920 - Pf1N1B4_1814 0.72 +0.5 2,085,920 - Pf1N1B4_1814 0.72 -2.1 2,085,920 - Pf1N1B4_1814 0.72 +0.5 2,085,920 - Pf1N1B4_1814 0.72 -0.1 2,085,920 - Pf1N1B4_1814 0.72 +0.2 2,085,922 + Pf1N1B4_1814 0.72 -1.1 2,085,923 - Pf1N1B4_1814 0.72 +2.1 2,085,923 - Pf1N1B4_1814 0.72 +0.5 2,085,923 - Pf1N1B4_1814 0.72 -1.5 2,085,943 + Pf1N1B4_1814 0.74 -0.7 2,086,139 + -1.1 2,086,139 + -2.3 2,086,139 + -1.1 2,086,139 + +0.8 2,086,157 + +0.5 2,086,157 + +1.5 2,086,158 - -0.2 2,086,158 - +1.5 2,086,177 + -0.5 2,086,177 + -1.8 2,086,177 + -2.1 2,086,177 + -1.0 2,086,177 + +1.8 2,086,177 + +1.8 2,086,177 + -0.2 2,086,177 + +0.5 2,086,177 + -1.1 2,086,177 + -0.8 2,086,177 + -0.5 2,086,177 + -0.8 2,086,177 + +0.5 2,086,177 + -1.4 2,086,177 + -1.5 2,086,177 + -1.7 2,086,177 + -0.1 2,086,177 + +0.2 2,086,177 + -1.5 2,086,177 + -1.5 2,086,177 + +1.5 2,086,177 + -2.3 2,086,178 - -1.1 2,086,178 - +1.5 2,086,178 - +1.5 2,086,178 - +0.5 2,086,179 + +0.5 2,086,212 + +0.1 2,086,213 - +0.2 2,086,224 + -0.8 2,086,224 + -1.3 2,086,224 + +0.5 2,086,224 + -0.4 2,086,224 + -1.1 2,086,224 + +1.5 2,086,224 + -0.3 2,086,224 + +1.1 2,086,225 - +1.3 2,086,261 + +0.5 2,086,261 + -0.2 2,086,261 + -0.3 2,086,261 + +2.1 2,086,293 + +1.1 2,086,294 - -0.5 2,086,335 - Pf1N1B4_5187 0.14 -0.1 2,086,335 - Pf1N1B4_5187 0.14 +0.8 2,086,593 - -0.3 2,086,595 - -1.8 2,086,595 - +1.3
Or see this region's nucleotide sequence