Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5158 and Pf1N1B4_5159 overlap by 25 nucleotides
Pf1N1B4_5158 - Endoglucanase precursor (EC 3.2.1.4), at 5,748,820 to 5,750,061
_5158
Pf1N1B4_5159 - Cellulose synthase operon protein C, at 5,750,037 to 5,753,897
_5159
Position (kb)
5750
5751
5752
5753
5754 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5749.435 kb on + strand, within Pf1N1B4_5158 at 5749.435 kb on + strand, within Pf1N1B4_5158 at 5749.436 kb on - strand, within Pf1N1B4_5158 at 5749.633 kb on + strand, within Pf1N1B4_5158 at 5749.634 kb on - strand, within Pf1N1B4_5158 at 5749.634 kb on - strand, within Pf1N1B4_5158 at 5749.651 kb on + strand, within Pf1N1B4_5158 at 5749.674 kb on + strand, within Pf1N1B4_5158 at 5749.942 kb on + strand at 5749.942 kb on + strand at 5749.943 kb on - strand at 5749.943 kb on - strand at 5749.943 kb on - strand at 5750.126 kb on - strand at 5750.232 kb on + strand at 5750.493 kb on + strand, within Pf1N1B4_5159 at 5750.494 kb on - strand at 5750.764 kb on - strand, within Pf1N1B4_5159 at 5751.261 kb on + strand, within Pf1N1B4_5159 at 5751.262 kb on - strand, within Pf1N1B4_5159 at 5751.636 kb on + strand, within Pf1N1B4_5159 at 5751.637 kb on - strand, within Pf1N1B4_5159 at 5751.637 kb on - strand, within Pf1N1B4_5159 at 5751.637 kb on - strand at 5751.889 kb on - strand, within Pf1N1B4_5159 at 5752.156 kb on - strand, within Pf1N1B4_5159 at 5752.279 kb on + strand at 5752.803 kb on + strand, within Pf1N1B4_5159 at 5752.803 kb on + strand, within Pf1N1B4_5159 at 5752.804 kb on - strand, within Pf1N1B4_5159 at 5752.804 kb on - strand at 5753.110 kb on - strand, within Pf1N1B4_5159 at 5753.484 kb on + strand at 5753.493 kb on + strand, within Pf1N1B4_5159 at 5753.494 kb on - strand, within Pf1N1B4_5159 at 5753.733 kb on + strand at 5753.733 kb on + strand at 5753.968 kb on + strand at 5753.969 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,749,435 + Pf1N1B4_5158 0.50 -1.6 5,749,435 + Pf1N1B4_5158 0.50 -1.8 5,749,436 - Pf1N1B4_5158 0.50 -2.6 5,749,633 + Pf1N1B4_5158 0.65 -2.2 5,749,634 - Pf1N1B4_5158 0.66 -1.0 5,749,634 - Pf1N1B4_5158 0.66 -1.0 5,749,651 + Pf1N1B4_5158 0.67 +0.0 5,749,674 + Pf1N1B4_5158 0.69 +1.2 5,749,942 + -1.2 5,749,942 + -0.6 5,749,943 - -0.6 5,749,943 - -1.2 5,749,943 - -0.6 5,750,126 - -0.2 5,750,232 + +1.2 5,750,493 + Pf1N1B4_5159 0.12 +0.4 5,750,494 - -0.6 5,750,764 - Pf1N1B4_5159 0.19 +0.4 5,751,261 + Pf1N1B4_5159 0.32 +0.6 5,751,262 - Pf1N1B4_5159 0.32 +0.4 5,751,636 + Pf1N1B4_5159 0.41 +0.4 5,751,637 - Pf1N1B4_5159 0.41 +0.4 5,751,637 - Pf1N1B4_5159 0.41 -0.6 5,751,637 - -0.6 5,751,889 - Pf1N1B4_5159 0.48 -0.9 5,752,156 - Pf1N1B4_5159 0.55 -0.7 5,752,279 + -1.6 5,752,803 + Pf1N1B4_5159 0.72 +0.6 5,752,803 + Pf1N1B4_5159 0.72 +2.2 5,752,804 - Pf1N1B4_5159 0.72 +0.7 5,752,804 - +1.0 5,753,110 - Pf1N1B4_5159 0.80 +0.8 5,753,484 + -2.6 5,753,493 + Pf1N1B4_5159 0.90 +0.7 5,753,494 - Pf1N1B4_5159 0.90 -1.6 5,753,733 + -0.1 5,753,733 + -1.0 5,753,968 + -0.7 5,753,969 - -0.8
Or see this region's nucleotide sequence