Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4936 and Pf1N1B4_4937 are separated by 102 nucleotides Pf1N1B4_4937 and Pf1N1B4_4938 are separated by 190 nucleotides Pf1N1B4_4938 and Pf1N1B4_4939 are separated by 205 nucleotides
Pf1N1B4_4936 - hypothetical protein, at 5,472,457 to 5,473,362
_4936
Pf1N1B4_4937 - Two-component hybrid sensor and regulator, at 5,473,465 to 5,475,384
_4937
Pf1N1B4_4938 - Regulator of cell morphogenesis and NO signaling, at 5,475,575 to 5,476,051
_4938
Pf1N1B4_4939 - sodium/hydrogen exchanger family protein, at 5,476,257 to 5,477,603
_4939
Position (kb) 5473
5474
5475
5476 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5472.475 kb on + strand at 5472.597 kb on + strand at 5472.649 kb on + strand, within Pf1N1B4_4936 at 5472.649 kb on + strand, within Pf1N1B4_4936 at 5472.662 kb on - strand, within Pf1N1B4_4936 at 5473.135 kb on + strand, within Pf1N1B4_4936 at 5473.135 kb on + strand, within Pf1N1B4_4936 at 5473.187 kb on - strand at 5473.187 kb on - strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.188 kb on + strand, within Pf1N1B4_4936 at 5473.189 kb on - strand, within Pf1N1B4_4936 at 5473.225 kb on + strand, within Pf1N1B4_4936 at 5473.226 kb on - strand, within Pf1N1B4_4936 at 5473.226 kb on - strand, within Pf1N1B4_4936 at 5473.292 kb on - strand at 5473.318 kb on + strand at 5473.318 kb on + strand at 5473.331 kb on - strand at 5473.336 kb on + strand at 5473.336 kb on + strand at 5473.337 kb on - strand at 5473.337 kb on - strand at 5473.348 kb on + strand at 5473.348 kb on + strand at 5473.348 kb on + strand at 5473.348 kb on + strand at 5473.391 kb on + strand at 5473.391 kb on + strand at 5473.392 kb on - strand at 5473.654 kb on + strand at 5473.655 kb on - strand at 5473.933 kb on + strand, within Pf1N1B4_4937 at 5473.933 kb on + strand, within Pf1N1B4_4937 at 5473.934 kb on - strand at 5474.038 kb on + strand, within Pf1N1B4_4937 at 5474.039 kb on - strand, within Pf1N1B4_4937 at 5474.359 kb on - strand, within Pf1N1B4_4937 at 5474.523 kb on - strand at 5474.585 kb on + strand, within Pf1N1B4_4937 at 5474.585 kb on + strand at 5474.586 kb on - strand, within Pf1N1B4_4937 at 5474.896 kb on + strand at 5474.896 kb on + strand, within Pf1N1B4_4937 at 5474.896 kb on + strand at 5474.897 kb on - strand, within Pf1N1B4_4937 at 5474.899 kb on - strand, within Pf1N1B4_4937 at 5475.155 kb on + strand, within Pf1N1B4_4937 at 5475.156 kb on - strand, within Pf1N1B4_4937 at 5475.399 kb on + strand at 5475.399 kb on + strand at 5475.550 kb on - strand at 5475.573 kb on - strand at 5475.841 kb on - strand, within Pf1N1B4_4938 at 5475.844 kb on - strand, within Pf1N1B4_4938 at 5475.844 kb on - strand, within Pf1N1B4_4938 at 5476.011 kb on - strand at 5476.069 kb on - strand at 5476.069 kb on - strand at 5476.307 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,472,475 + -0.6 5,472,597 + +1.4 5,472,649 + Pf1N1B4_4936 0.21 -2.1 5,472,649 + Pf1N1B4_4936 0.21 -1.6 5,472,662 - Pf1N1B4_4936 0.23 -1.4 5,473,135 + Pf1N1B4_4936 0.75 -0.9 5,473,135 + Pf1N1B4_4936 0.75 -1.8 5,473,187 - -0.6 5,473,187 - Pf1N1B4_4936 0.81 +0.5 5,473,188 + Pf1N1B4_4936 0.81 -0.3 5,473,188 + Pf1N1B4_4936 0.81 -1.9 5,473,188 + Pf1N1B4_4936 0.81 +0.4 5,473,189 - Pf1N1B4_4936 0.81 -0.6 5,473,225 + Pf1N1B4_4936 0.85 -0.9 5,473,226 - Pf1N1B4_4936 0.85 +0.7 5,473,226 - Pf1N1B4_4936 0.85 +1.2 5,473,292 - +0.2 5,473,318 + +1.0 5,473,318 + -0.6 5,473,331 - -0.6 5,473,336 + -2.2 5,473,336 + -2.6 5,473,337 - -0.6 5,473,337 - +1.0 5,473,348 + -1.6 5,473,348 + -2.1 5,473,348 + +0.4 5,473,348 + -0.7 5,473,391 + -0.6 5,473,391 + -1.0 5,473,392 - +0.7 5,473,654 + -0.6 5,473,655 - +0.3 5,473,933 + Pf1N1B4_4937 0.24 +1.2 5,473,933 + Pf1N1B4_4937 0.24 +2.9 5,473,934 - -0.6 5,474,038 + Pf1N1B4_4937 0.30 +1.3 5,474,039 - Pf1N1B4_4937 0.30 +0.4 5,474,359 - Pf1N1B4_4937 0.47 +1.7 5,474,523 - -0.6 5,474,585 + Pf1N1B4_4937 0.58 +1.0 5,474,585 + +1.4 5,474,586 - Pf1N1B4_4937 0.58 +0.6 5,474,896 + -1.8 5,474,896 + Pf1N1B4_4937 0.75 +1.4 5,474,896 + -1.6 5,474,897 - Pf1N1B4_4937 0.75 +0.2 5,474,899 - Pf1N1B4_4937 0.75 -1.6 5,475,155 + Pf1N1B4_4937 0.88 +0.4 5,475,156 - Pf1N1B4_4937 0.88 +1.1 5,475,399 + +1.0 5,475,399 + -0.6 5,475,550 - +0.1 5,475,573 - -0.6 5,475,841 - Pf1N1B4_4938 0.56 -0.6 5,475,844 - Pf1N1B4_4938 0.56 -0.6 5,475,844 - Pf1N1B4_4938 0.56 +0.4 5,476,011 - -2.2 5,476,069 - -0.3 5,476,069 - -1.0 5,476,307 + -0.6
Or see this region's nucleotide sequence