Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4896 and Pf1N1B4_4897 are separated by 224 nucleotides Pf1N1B4_4897 and Pf1N1B4_4898 are separated by 99 nucleotides Pf1N1B4_4898 and Pf1N1B4_4899 are separated by 654 nucleotides
Pf1N1B4_4896 - N-ethylmaleimide reductase, at 5,418,216 to 5,419,238
_4896
Pf1N1B4_4897 - Transcriptional regulator, LysR family, at 5,419,463 to 5,420,347
_4897
Pf1N1B4_4898 - Alcohol dehydrogenase (EC 1.1.1.1), at 5,420,447 to 5,420,560
_4898
Pf1N1B4_4899 - Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog, at 5,421,215 to 5,422,672
_4899
Position (kb) 5419
5420
5421 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5418.510 kb on + strand, within Pf1N1B4_4896 at 5418.539 kb on - strand, within Pf1N1B4_4896 at 5418.558 kb on + strand, within Pf1N1B4_4896 at 5418.608 kb on + strand, within Pf1N1B4_4896 at 5418.681 kb on + strand, within Pf1N1B4_4896 at 5418.682 kb on - strand, within Pf1N1B4_4896 at 5418.727 kb on + strand, within Pf1N1B4_4896 at 5418.728 kb on - strand, within Pf1N1B4_4896 at 5418.728 kb on - strand, within Pf1N1B4_4896 at 5418.738 kb on - strand, within Pf1N1B4_4896 at 5418.984 kb on + strand at 5419.016 kb on - strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.097 kb on - strand, within Pf1N1B4_4896 at 5419.097 kb on - strand, within Pf1N1B4_4896 at 5419.097 kb on - strand at 5419.179 kb on - strand at 5419.179 kb on - strand at 5419.179 kb on - strand at 5419.189 kb on + strand at 5419.190 kb on - strand at 5419.192 kb on + strand at 5419.287 kb on + strand at 5419.288 kb on - strand at 5419.301 kb on + strand at 5419.329 kb on + strand at 5419.330 kb on - strand at 5419.330 kb on - strand at 5419.354 kb on + strand at 5419.361 kb on - strand at 5419.441 kb on + strand at 5419.600 kb on - strand, within Pf1N1B4_4897 at 5419.600 kb on - strand at 5419.762 kb on - strand at 5420.145 kb on - strand at 5420.145 kb on - strand, within Pf1N1B4_4897 at 5420.240 kb on + strand at 5420.241 kb on - strand, within Pf1N1B4_4897 at 5420.241 kb on - strand, within Pf1N1B4_4897 at 5420.321 kb on + strand at 5420.321 kb on + strand at 5420.322 kb on - strand at 5420.495 kb on + strand at 5420.496 kb on - strand, within Pf1N1B4_4898 at 5420.496 kb on - strand, within Pf1N1B4_4898 at 5420.496 kb on - strand, within Pf1N1B4_4898 at 5420.496 kb on - strand, within Pf1N1B4_4898 at 5420.562 kb on - strand at 5420.562 kb on - strand at 5420.562 kb on - strand at 5420.610 kb on - strand at 5420.610 kb on - strand at 5420.610 kb on - strand at 5420.610 kb on - strand at 5420.610 kb on - strand at 5420.614 kb on - strand at 5420.615 kb on + strand at 5420.665 kb on - strand at 5420.694 kb on - strand at 5420.911 kb on - strand at 5421.019 kb on + strand at 5421.021 kb on + strand at 5421.299 kb on + strand at 5421.329 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,418,510 + Pf1N1B4_4896 0.29 +0.4 5,418,539 - Pf1N1B4_4896 0.32 +0.3 5,418,558 + Pf1N1B4_4896 0.33 +0.4 5,418,608 + Pf1N1B4_4896 0.38 +0.6 5,418,681 + Pf1N1B4_4896 0.45 -0.6 5,418,682 - Pf1N1B4_4896 0.46 -1.6 5,418,727 + Pf1N1B4_4896 0.50 -0.1 5,418,728 - Pf1N1B4_4896 0.50 -0.6 5,418,728 - Pf1N1B4_4896 0.50 -0.3 5,418,738 - Pf1N1B4_4896 0.51 +0.7 5,418,984 + +0.4 5,419,016 - Pf1N1B4_4896 0.78 -0.0 5,419,096 + Pf1N1B4_4896 0.86 -0.0 5,419,096 + Pf1N1B4_4896 0.86 -1.0 5,419,096 + Pf1N1B4_4896 0.86 -2.0 5,419,097 - Pf1N1B4_4896 0.86 -0.7 5,419,097 - Pf1N1B4_4896 0.86 -0.1 5,419,097 - -1.2 5,419,179 - +0.6 5,419,179 - -0.0 5,419,179 - +1.4 5,419,189 + -1.6 5,419,190 - -0.6 5,419,192 + -1.6 5,419,287 + -0.6 5,419,288 - +1.0 5,419,301 + -0.6 5,419,329 + -1.6 5,419,330 - +1.0 5,419,330 - +1.7 5,419,354 + +0.5 5,419,361 - -0.6 5,419,441 + -0.6 5,419,600 - Pf1N1B4_4897 0.15 -2.6 5,419,600 - -2.2 5,419,762 - -1.6 5,420,145 - -0.2 5,420,145 - Pf1N1B4_4897 0.77 -0.6 5,420,240 + +0.4 5,420,241 - Pf1N1B4_4897 0.88 -3.2 5,420,241 - Pf1N1B4_4897 0.88 +0.1 5,420,321 + -0.3 5,420,321 + -1.2 5,420,322 - -0.8 5,420,495 + -2.6 5,420,496 - Pf1N1B4_4898 0.43 +0.4 5,420,496 - Pf1N1B4_4898 0.43 -0.0 5,420,496 - Pf1N1B4_4898 0.43 -0.6 5,420,496 - Pf1N1B4_4898 0.43 -1.0 5,420,562 - +0.5 5,420,562 - -1.1 5,420,562 - -1.6 5,420,610 - +0.4 5,420,610 - -2.2 5,420,610 - -1.6 5,420,610 - -1.2 5,420,610 - -0.6 5,420,614 - -0.6 5,420,615 + +2.2 5,420,665 - -1.2 5,420,694 - +0.4 5,420,911 - +0.4 5,421,019 + -0.0 5,421,021 + +1.0 5,421,299 + -0.2 5,421,329 + -1.8
Or see this region's nucleotide sequence