Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4863 and Pf1N1B4_4864 overlap by 4 nucleotides Pf1N1B4_4864 and Pf1N1B4_4865 are separated by 12 nucleotides
Pf1N1B4_4863 - Outer membrane protein OprN, at 5,378,345 to 5,379,766
_4863
Pf1N1B4_4864 - Multidrug efflux transporter MexF, at 5,379,763 to 5,382,954
_4864
Pf1N1B4_4865 - Multidrug efflux membrane fusion protein MexE, at 5,382,967 to 5,384,220
_4865
Position (kb) 5379
5380
5381
5382
5383 Strain fitness (log2 ratio) -2
-1
0
1 at 5379.232 kb on - strand, within Pf1N1B4_4863 at 5379.379 kb on - strand, within Pf1N1B4_4863 at 5379.851 kb on - strand at 5379.851 kb on - strand at 5379.854 kb on + strand at 5379.854 kb on + strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5380.110 kb on + strand, within Pf1N1B4_4864 at 5380.111 kb on - strand, within Pf1N1B4_4864 at 5380.111 kb on - strand at 5380.254 kb on - strand, within Pf1N1B4_4864 at 5380.286 kb on - strand, within Pf1N1B4_4864 at 5380.337 kb on + strand at 5380.338 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.625 kb on + strand, within Pf1N1B4_4864 at 5380.625 kb on + strand, within Pf1N1B4_4864 at 5380.658 kb on + strand, within Pf1N1B4_4864 at 5380.673 kb on + strand, within Pf1N1B4_4864 at 5380.772 kb on + strand, within Pf1N1B4_4864 at 5380.773 kb on - strand, within Pf1N1B4_4864 at 5380.773 kb on - strand, within Pf1N1B4_4864 at 5380.841 kb on + strand, within Pf1N1B4_4864 at 5380.842 kb on - strand, within Pf1N1B4_4864 at 5380.943 kb on + strand, within Pf1N1B4_4864 at 5380.943 kb on + strand, within Pf1N1B4_4864 at 5380.944 kb on - strand, within Pf1N1B4_4864 at 5380.944 kb on - strand, within Pf1N1B4_4864 at 5381.149 kb on - strand, within Pf1N1B4_4864 at 5381.149 kb on - strand, within Pf1N1B4_4864 at 5381.149 kb on - strand, within Pf1N1B4_4864 at 5381.217 kb on - strand, within Pf1N1B4_4864 at 5381.336 kb on - strand, within Pf1N1B4_4864 at 5381.349 kb on - strand, within Pf1N1B4_4864 at 5381.349 kb on - strand, within Pf1N1B4_4864 at 5381.349 kb on - strand at 5381.548 kb on - strand, within Pf1N1B4_4864 at 5381.549 kb on + strand at 5381.890 kb on + strand, within Pf1N1B4_4864 at 5381.966 kb on + strand, within Pf1N1B4_4864 at 5381.966 kb on + strand, within Pf1N1B4_4864 at 5382.196 kb on - strand, within Pf1N1B4_4864 at 5382.479 kb on + strand, within Pf1N1B4_4864 at 5382.480 kb on - strand at 5382.480 kb on - strand, within Pf1N1B4_4864 at 5382.491 kb on + strand, within Pf1N1B4_4864 at 5382.492 kb on - strand, within Pf1N1B4_4864 at 5382.719 kb on + strand at 5382.719 kb on + strand at 5382.719 kb on + strand at 5382.719 kb on + strand at 5382.719 kb on + strand at 5382.720 kb on - strand at 5382.720 kb on - strand at 5382.957 kb on + strand at 5382.957 kb on + strand at 5383.331 kb on + strand, within Pf1N1B4_4865 at 5383.554 kb on - strand, within Pf1N1B4_4865 at 5383.557 kb on + strand, within Pf1N1B4_4865 at 5383.558 kb on - strand, within Pf1N1B4_4865 at 5383.558 kb on - strand, within Pf1N1B4_4865 at 5383.689 kb on + strand, within Pf1N1B4_4865
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,379,232 - Pf1N1B4_4863 0.62 +0.4 5,379,379 - Pf1N1B4_4863 0.73 -1.2 5,379,851 - -1.6 5,379,851 - -1.0 5,379,854 + -0.0 5,379,854 + -0.2 5,379,855 - +0.4 5,379,855 - -1.6 5,379,855 - -1.1 5,379,855 - -0.6 5,379,855 - -0.2 5,379,855 - -0.1 5,379,855 - +0.1 5,380,110 + Pf1N1B4_4864 0.11 -0.0 5,380,111 - Pf1N1B4_4864 0.11 -0.7 5,380,111 - -2.6 5,380,254 - Pf1N1B4_4864 0.15 +0.1 5,380,286 - Pf1N1B4_4864 0.16 +1.2 5,380,337 + -1.6 5,380,338 - Pf1N1B4_4864 0.18 +1.7 5,380,476 - Pf1N1B4_4864 0.22 +0.1 5,380,476 - Pf1N1B4_4864 0.22 -2.2 5,380,476 - Pf1N1B4_4864 0.22 -2.0 5,380,476 - Pf1N1B4_4864 0.22 -1.0 5,380,625 + Pf1N1B4_4864 0.27 -1.6 5,380,625 + Pf1N1B4_4864 0.27 -2.2 5,380,658 + Pf1N1B4_4864 0.28 -1.9 5,380,673 + Pf1N1B4_4864 0.29 -1.6 5,380,772 + Pf1N1B4_4864 0.32 +0.0 5,380,773 - Pf1N1B4_4864 0.32 +0.4 5,380,773 - Pf1N1B4_4864 0.32 +0.4 5,380,841 + Pf1N1B4_4864 0.34 +0.6 5,380,842 - Pf1N1B4_4864 0.34 -2.4 5,380,943 + Pf1N1B4_4864 0.37 -0.6 5,380,943 + Pf1N1B4_4864 0.37 -2.6 5,380,944 - Pf1N1B4_4864 0.37 -1.0 5,380,944 - Pf1N1B4_4864 0.37 -2.2 5,381,149 - Pf1N1B4_4864 0.43 -1.9 5,381,149 - Pf1N1B4_4864 0.43 -0.0 5,381,149 - Pf1N1B4_4864 0.43 -0.8 5,381,217 - Pf1N1B4_4864 0.46 -0.6 5,381,336 - Pf1N1B4_4864 0.49 -1.0 5,381,349 - Pf1N1B4_4864 0.50 -1.0 5,381,349 - Pf1N1B4_4864 0.50 -0.6 5,381,349 - -0.6 5,381,548 - Pf1N1B4_4864 0.56 +0.4 5,381,549 + +1.0 5,381,890 + Pf1N1B4_4864 0.67 -0.6 5,381,966 + Pf1N1B4_4864 0.69 -1.0 5,381,966 + Pf1N1B4_4864 0.69 -1.6 5,382,196 - Pf1N1B4_4864 0.76 +0.1 5,382,479 + Pf1N1B4_4864 0.85 -0.6 5,382,480 - -1.6 5,382,480 - Pf1N1B4_4864 0.85 -0.6 5,382,491 + Pf1N1B4_4864 0.85 +1.2 5,382,492 - Pf1N1B4_4864 0.85 +0.7 5,382,719 + -0.6 5,382,719 + +1.2 5,382,719 + +0.6 5,382,719 + +1.0 5,382,719 + -0.6 5,382,720 - +1.7 5,382,720 - +1.4 5,382,957 + +0.4 5,382,957 + +0.9 5,383,331 + Pf1N1B4_4865 0.29 -0.6 5,383,554 - Pf1N1B4_4865 0.47 -1.9 5,383,557 + Pf1N1B4_4865 0.47 +0.1 5,383,558 - Pf1N1B4_4865 0.47 -1.6 5,383,558 - Pf1N1B4_4865 0.47 -0.1 5,383,689 + Pf1N1B4_4865 0.58 +0.2
Or see this region's nucleotide sequence