Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4790

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4789 and Pf1N1B4_4790 are separated by 3 nucleotidesPf1N1B4_4790 and Pf1N1B4_4791 are separated by 598 nucleotides Pf1N1B4_4789 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 5,291,697 to 5,292,848 _4789 Pf1N1B4_4790 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data), at 5,292,852 to 5,293,922 _4790 Pf1N1B4_4791 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 5,294,521 to 5,295,300 _4791 Position (kb) 5292 5293 5294Strain fitness (log2 ratio) -2 -1 0 1at 5293.182 kb on - strand, within Pf1N1B4_4790at 5293.366 kb on + strand, within Pf1N1B4_4790at 5293.388 kb on - strand, within Pf1N1B4_4790at 5293.388 kb on - strand, within Pf1N1B4_4790at 5293.388 kb on - strand, within Pf1N1B4_4790at 5293.432 kb on + strand, within Pf1N1B4_4790at 5293.432 kb on + strand, within Pf1N1B4_4790at 5293.433 kb on - strand, within Pf1N1B4_4790at 5293.433 kb on - strand, within Pf1N1B4_4790at 5293.649 kb on - strand, within Pf1N1B4_4790at 5293.976 kb on + strandat 5294.053 kb on + strandat 5294.251 kb on - strandat 5294.295 kb on - strandat 5294.416 kb on + strandat 5294.493 kb on - strandat 5294.503 kb on - strandat 5294.515 kb on + strandat 5294.516 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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5,293,182 - Pf1N1B4_4790 0.31 -0.5
5,293,366 + Pf1N1B4_4790 0.48 -1.2
5,293,388 - Pf1N1B4_4790 0.50 -1.9
5,293,388 - Pf1N1B4_4790 0.50 -2.3
5,293,388 - Pf1N1B4_4790 0.50 +0.0
5,293,432 + Pf1N1B4_4790 0.54 -1.2
5,293,432 + Pf1N1B4_4790 0.54 -1.8
5,293,433 - Pf1N1B4_4790 0.54 -1.0
5,293,433 - Pf1N1B4_4790 0.54 +0.4
5,293,649 - Pf1N1B4_4790 0.74 -2.2
5,293,976 + -1.6
5,294,053 + +0.0
5,294,251 - -0.6
5,294,295 - -1.2
5,294,416 + -0.8
5,294,493 - -1.9
5,294,503 - +0.4
5,294,515 + +1.4
5,294,516 - -0.2

Or see this region's nucleotide sequence