Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4787

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4786 and Pf1N1B4_4787 are separated by 165 nucleotidesPf1N1B4_4787 and Pf1N1B4_4788 are separated by 14 nucleotidesPf1N1B4_4788 and Pf1N1B4_4789 are separated by 136 nucleotides Pf1N1B4_4786 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 5,288,286 to 5,289,479 _4786 Pf1N1B4_4787 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data), at 5,289,645 to 5,290,772 _4787 Pf1N1B4_4788 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,290,787 to 5,291,560 _4788 Pf1N1B4_4789 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 5,291,697 to 5,292,848 _4789 Position (kb) 5289 5290 5291Strain fitness (log2 ratio) -3 -2 -1 0 1at 5289.036 kb on - strandat 5289.474 kb on + strandat 5289.474 kb on + strandat 5289.617 kb on + strandat 5289.761 kb on + strandat 5289.762 kb on - strand, within Pf1N1B4_4787at 5289.917 kb on - strand, within Pf1N1B4_4787at 5290.080 kb on + strand, within Pf1N1B4_4787at 5290.080 kb on + strand, within Pf1N1B4_4787at 5290.215 kb on + strand, within Pf1N1B4_4787at 5290.218 kb on - strand, within Pf1N1B4_4787at 5290.218 kb on - strandat 5290.262 kb on + strand, within Pf1N1B4_4787at 5290.539 kb on + strand, within Pf1N1B4_4787at 5290.566 kb on - strand, within Pf1N1B4_4787at 5290.722 kb on + strandat 5290.723 kb on - strandat 5290.723 kb on - strandat 5290.723 kb on - strandat 5290.786 kb on + strandat 5290.786 kb on + strandat 5290.787 kb on - strandat 5290.787 kb on - strandat 5290.787 kb on - strandat 5290.787 kb on - strandat 5290.794 kb on - strandat 5291.021 kb on + strand, within Pf1N1B4_4788at 5291.022 kb on - strand, within Pf1N1B4_4788at 5291.525 kb on - strandat 5291.558 kb on + strandat 5291.558 kb on + strandat 5291.708 kb on - strandat 5291.708 kb on - strandat 5291.743 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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5,289,036 - -0.6
5,289,474 + -3.2
5,289,474 + -2.0
5,289,617 + -0.6
5,289,761 + -1.6
5,289,762 - Pf1N1B4_4787 0.10 -0.6
5,289,917 - Pf1N1B4_4787 0.24 -2.9
5,290,080 + Pf1N1B4_4787 0.39 -2.6
5,290,080 + Pf1N1B4_4787 0.39 -1.6
5,290,215 + Pf1N1B4_4787 0.51 -1.6
5,290,218 - Pf1N1B4_4787 0.51 -1.6
5,290,218 - -1.6
5,290,262 + Pf1N1B4_4787 0.55 -3.4
5,290,539 + Pf1N1B4_4787 0.79 -1.6
5,290,566 - Pf1N1B4_4787 0.82 -2.9
5,290,722 + +0.4
5,290,723 - -2.2
5,290,723 - -2.9
5,290,723 - -2.6
5,290,786 + -2.9
5,290,786 + -3.6
5,290,787 - -1.9
5,290,787 - -2.9
5,290,787 - -0.6
5,290,787 - -2.2
5,290,794 - -2.6
5,291,021 + Pf1N1B4_4788 0.30 -2.9
5,291,022 - Pf1N1B4_4788 0.30 -2.2
5,291,525 - -2.6
5,291,558 + -0.6
5,291,558 + -1.3
5,291,708 - -0.0
5,291,708 - -0.6
5,291,743 - +1.0

Or see this region's nucleotide sequence