Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4786 and Pf1N1B4_4787 are separated by 165 nucleotides Pf1N1B4_4787 and Pf1N1B4_4788 are separated by 14 nucleotides Pf1N1B4_4788 and Pf1N1B4_4789 are separated by 136 nucleotides
Pf1N1B4_4786 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 5,288,286 to 5,289,479
_4786
Pf1N1B4_4787 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 5,289,645 to 5,290,772
_4787
Pf1N1B4_4788 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,290,787 to 5,291,560
_4788
Pf1N1B4_4789 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 5,291,697 to 5,292,848
_4789
Position (kb)
5289
5290
5291 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5289.036 kb on - strand at 5289.474 kb on + strand at 5289.474 kb on + strand at 5289.617 kb on + strand at 5289.761 kb on + strand at 5289.762 kb on - strand, within Pf1N1B4_4787 at 5289.917 kb on - strand, within Pf1N1B4_4787 at 5290.080 kb on + strand, within Pf1N1B4_4787 at 5290.080 kb on + strand, within Pf1N1B4_4787 at 5290.215 kb on + strand, within Pf1N1B4_4787 at 5290.218 kb on - strand, within Pf1N1B4_4787 at 5290.218 kb on - strand at 5290.262 kb on + strand, within Pf1N1B4_4787 at 5290.539 kb on + strand, within Pf1N1B4_4787 at 5290.566 kb on - strand, within Pf1N1B4_4787 at 5290.722 kb on + strand at 5290.723 kb on - strand at 5290.723 kb on - strand at 5290.723 kb on - strand at 5290.786 kb on + strand at 5290.786 kb on + strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.794 kb on - strand at 5291.021 kb on + strand, within Pf1N1B4_4788 at 5291.022 kb on - strand, within Pf1N1B4_4788 at 5291.525 kb on - strand at 5291.558 kb on + strand at 5291.558 kb on + strand at 5291.708 kb on - strand at 5291.708 kb on - strand at 5291.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,289,036 - -0.6 5,289,474 + -3.2 5,289,474 + -2.0 5,289,617 + -0.6 5,289,761 + -1.6 5,289,762 - Pf1N1B4_4787 0.10 -0.6 5,289,917 - Pf1N1B4_4787 0.24 -2.9 5,290,080 + Pf1N1B4_4787 0.39 -2.6 5,290,080 + Pf1N1B4_4787 0.39 -1.6 5,290,215 + Pf1N1B4_4787 0.51 -1.6 5,290,218 - Pf1N1B4_4787 0.51 -1.6 5,290,218 - -1.6 5,290,262 + Pf1N1B4_4787 0.55 -3.4 5,290,539 + Pf1N1B4_4787 0.79 -1.6 5,290,566 - Pf1N1B4_4787 0.82 -2.9 5,290,722 + +0.4 5,290,723 - -2.2 5,290,723 - -2.9 5,290,723 - -2.6 5,290,786 + -2.9 5,290,786 + -3.6 5,290,787 - -1.9 5,290,787 - -2.9 5,290,787 - -0.6 5,290,787 - -2.2 5,290,794 - -2.6 5,291,021 + Pf1N1B4_4788 0.30 -2.9 5,291,022 - Pf1N1B4_4788 0.30 -2.2 5,291,525 - -2.6 5,291,558 + -0.6 5,291,558 + -1.3 5,291,708 - -0.0 5,291,708 - -0.6 5,291,743 - +1.0
Or see this region's nucleotide sequence