Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4783 and Pf1N1B4_4784 are separated by 254 nucleotides Pf1N1B4_4784 and Pf1N1B4_4785 are separated by 16 nucleotides Pf1N1B4_4785 and Pf1N1B4_4786 are separated by 12 nucleotides
Pf1N1B4_4783 - Transcriptional regulator, AraC family, at 5,284,560 to 5,285,576
_4783
Pf1N1B4_4784 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 5,285,831 to 5,287,495
_4784
Pf1N1B4_4785 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 5,287,512 to 5,288,273
_4785
Pf1N1B4_4786 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 5,288,286 to 5,289,479
_4786
Position (kb) 5285
5286
5287
5288 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5284.974 kb on + strand, within Pf1N1B4_4783 at 5284.974 kb on + strand, within Pf1N1B4_4783 at 5284.975 kb on - strand at 5284.984 kb on - strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.502 kb on + strand at 5285.502 kb on + strand at 5285.531 kb on + strand at 5285.574 kb on + strand at 5285.574 kb on + strand at 5285.574 kb on + strand at 5285.697 kb on + strand at 5285.697 kb on + strand at 5285.698 kb on - strand at 5285.698 kb on - strand at 5285.698 kb on - strand at 5285.702 kb on - strand at 5285.702 kb on - strand at 5285.788 kb on + strand at 5285.788 kb on + strand at 5285.789 kb on - strand at 5285.789 kb on - strand at 5285.804 kb on + strand at 5285.841 kb on - strand at 5285.841 kb on - strand at 5285.867 kb on + strand at 5285.867 kb on + strand at 5286.488 kb on + strand at 5286.566 kb on + strand, within Pf1N1B4_4784 at 5286.566 kb on + strand, within Pf1N1B4_4784 at 5286.567 kb on - strand, within Pf1N1B4_4784 at 5286.614 kb on + strand, within Pf1N1B4_4784 at 5286.615 kb on - strand, within Pf1N1B4_4784 at 5286.662 kb on + strand, within Pf1N1B4_4784 at 5286.662 kb on + strand, within Pf1N1B4_4784 at 5286.963 kb on - strand, within Pf1N1B4_4784 at 5287.076 kb on + strand, within Pf1N1B4_4784 at 5287.076 kb on + strand, within Pf1N1B4_4784 at 5287.077 kb on - strand, within Pf1N1B4_4784 at 5287.079 kb on + strand, within Pf1N1B4_4784 at 5287.432 kb on + strand at 5288.182 kb on - strand, within Pf1N1B4_4785 at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.202 kb on - strand at 5288.206 kb on + strand at 5288.206 kb on + strand at 5288.271 kb on + strand at 5288.272 kb on - strand at 5288.272 kb on - strand at 5288.281 kb on + strand at 5288.282 kb on - strand at 5288.282 kb on - strand at 5288.282 kb on - strand at 5288.312 kb on + strand at 5288.313 kb on - strand at 5288.313 kb on - strand at 5288.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,284,974 + Pf1N1B4_4783 0.41 -0.6 5,284,974 + Pf1N1B4_4783 0.41 -0.6 5,284,975 - -0.6 5,284,984 - Pf1N1B4_4783 0.42 -1.2 5,285,385 + Pf1N1B4_4783 0.81 -3.4 5,285,385 + Pf1N1B4_4783 0.81 -2.9 5,285,385 + Pf1N1B4_4783 0.81 -4.5 5,285,502 + -1.2 5,285,502 + -2.2 5,285,531 + -2.6 5,285,574 + -0.4 5,285,574 + +0.4 5,285,574 + -0.2 5,285,697 + +0.6 5,285,697 + -0.6 5,285,698 - -0.6 5,285,698 - -0.5 5,285,698 - -1.1 5,285,702 - -0.1 5,285,702 - -0.6 5,285,788 + -0.6 5,285,788 + -2.8 5,285,789 - -2.2 5,285,789 - -0.6 5,285,804 + -2.6 5,285,841 - -1.6 5,285,841 - -2.6 5,285,867 + -2.2 5,285,867 + -2.6 5,286,488 + -0.6 5,286,566 + Pf1N1B4_4784 0.44 -2.2 5,286,566 + Pf1N1B4_4784 0.44 -2.2 5,286,567 - Pf1N1B4_4784 0.44 -2.9 5,286,614 + Pf1N1B4_4784 0.47 -1.6 5,286,615 - Pf1N1B4_4784 0.47 -2.2 5,286,662 + Pf1N1B4_4784 0.50 -2.4 5,286,662 + Pf1N1B4_4784 0.50 -2.2 5,286,963 - Pf1N1B4_4784 0.68 -2.2 5,287,076 + Pf1N1B4_4784 0.75 -1.6 5,287,076 + Pf1N1B4_4784 0.75 -0.9 5,287,077 - Pf1N1B4_4784 0.75 -1.6 5,287,079 + Pf1N1B4_4784 0.75 -2.0 5,287,432 + -2.2 5,288,182 - Pf1N1B4_4785 0.88 -2.2 5,288,202 - -2.2 5,288,202 - -2.2 5,288,202 - -2.9 5,288,202 - -1.0 5,288,206 + -1.6 5,288,206 + -0.6 5,288,271 + +0.4 5,288,272 - -3.4 5,288,272 - -2.2 5,288,281 + -5.7 5,288,282 - -5.7 5,288,282 - +0.4 5,288,282 - -1.2 5,288,312 + -4.3 5,288,313 - -2.2 5,288,313 - -2.2 5,288,313 - -2.9
Or see this region's nucleotide sequence