Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4782 and Pf1N1B4_4783 are separated by 157 nucleotides Pf1N1B4_4783 and Pf1N1B4_4784 are separated by 254 nucleotides
Pf1N1B4_4782 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 5,283,059 to 5,284,402
_4782
Pf1N1B4_4783 - Transcriptional regulator, AraC family, at 5,284,560 to 5,285,576
_4783
Pf1N1B4_4784 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 5,285,831 to 5,287,495
_4784
Position (kb)
5284
5285
5286 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5283.722 kb on + strand, within Pf1N1B4_4782 at 5283.723 kb on - strand, within Pf1N1B4_4782 at 5284.010 kb on + strand, within Pf1N1B4_4782 at 5284.112 kb on + strand, within Pf1N1B4_4782 at 5284.113 kb on - strand, within Pf1N1B4_4782 at 5284.113 kb on - strand, within Pf1N1B4_4782 at 5284.475 kb on + strand at 5284.519 kb on + strand at 5284.519 kb on + strand at 5284.520 kb on - strand at 5284.714 kb on - strand, within Pf1N1B4_4783 at 5284.974 kb on + strand, within Pf1N1B4_4783 at 5284.974 kb on + strand, within Pf1N1B4_4783 at 5284.975 kb on - strand at 5284.984 kb on - strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.502 kb on + strand at 5285.502 kb on + strand at 5285.531 kb on + strand at 5285.574 kb on + strand at 5285.574 kb on + strand at 5285.574 kb on + strand at 5285.697 kb on + strand at 5285.697 kb on + strand at 5285.698 kb on - strand at 5285.698 kb on - strand at 5285.698 kb on - strand at 5285.702 kb on - strand at 5285.702 kb on - strand at 5285.788 kb on + strand at 5285.788 kb on + strand at 5285.789 kb on - strand at 5285.789 kb on - strand at 5285.804 kb on + strand at 5285.841 kb on - strand at 5285.841 kb on - strand at 5285.867 kb on + strand at 5285.867 kb on + strand at 5286.488 kb on + strand at 5286.566 kb on + strand, within Pf1N1B4_4784 at 5286.566 kb on + strand, within Pf1N1B4_4784 at 5286.567 kb on - strand, within Pf1N1B4_4784
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,283,722 + Pf1N1B4_4782 0.49 +0.4 5,283,723 - Pf1N1B4_4782 0.49 -1.6 5,284,010 + Pf1N1B4_4782 0.71 +0.2 5,284,112 + Pf1N1B4_4782 0.78 -0.9 5,284,113 - Pf1N1B4_4782 0.78 -0.6 5,284,113 - Pf1N1B4_4782 0.78 +0.8 5,284,475 + +0.6 5,284,519 + -0.6 5,284,519 + -0.8 5,284,520 - -1.9 5,284,714 - Pf1N1B4_4783 0.15 -3.2 5,284,974 + Pf1N1B4_4783 0.41 -0.6 5,284,974 + Pf1N1B4_4783 0.41 -0.6 5,284,975 - -0.6 5,284,984 - Pf1N1B4_4783 0.42 -1.2 5,285,385 + Pf1N1B4_4783 0.81 -3.4 5,285,385 + Pf1N1B4_4783 0.81 -2.9 5,285,385 + Pf1N1B4_4783 0.81 -4.5 5,285,502 + -1.2 5,285,502 + -2.2 5,285,531 + -2.6 5,285,574 + -0.4 5,285,574 + +0.4 5,285,574 + -0.2 5,285,697 + +0.6 5,285,697 + -0.6 5,285,698 - -0.6 5,285,698 - -0.5 5,285,698 - -1.1 5,285,702 - -0.1 5,285,702 - -0.6 5,285,788 + -0.6 5,285,788 + -2.8 5,285,789 - -2.2 5,285,789 - -0.6 5,285,804 + -2.6 5,285,841 - -1.6 5,285,841 - -2.6 5,285,867 + -2.2 5,285,867 + -2.6 5,286,488 + -0.6 5,286,566 + Pf1N1B4_4784 0.44 -2.2 5,286,566 + Pf1N1B4_4784 0.44 -2.2 5,286,567 - Pf1N1B4_4784 0.44 -2.9
Or see this region's nucleotide sequence