Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4677 and Pf1N1B4_4678 are separated by 2 nucleotides Pf1N1B4_4678 and Pf1N1B4_4679 are separated by 132 nucleotides
Pf1N1B4_4677 - Trehalose synthase (EC 5.4.99.16), at 5,159,775 to 5,163,116
_4677
Pf1N1B4_4678 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 5,163,119 to 5,165,350
_4678
Pf1N1B4_4679 - outer membrane autotransporter, at 5,165,483 to 5,166,550
_4679
Position (kb)
5163
5164
5165
5166 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5162.323 kb on - strand, within Pf1N1B4_4677 at 5162.755 kb on - strand, within Pf1N1B4_4677 at 5162.756 kb on + strand, within Pf1N1B4_4677 at 5162.756 kb on + strand at 5162.756 kb on + strand, within Pf1N1B4_4677 at 5162.756 kb on + strand at 5162.756 kb on + strand, within Pf1N1B4_4677 at 5162.756 kb on + strand, within Pf1N1B4_4677 at 5162.757 kb on - strand, within Pf1N1B4_4677 at 5162.757 kb on - strand, within Pf1N1B4_4677 at 5162.911 kb on - strand at 5162.927 kb on + strand at 5162.927 kb on + strand at 5163.064 kb on + strand at 5163.067 kb on - strand at 5163.114 kb on + strand at 5163.124 kb on + strand at 5163.124 kb on + strand at 5163.124 kb on + strand at 5163.444 kb on - strand, within Pf1N1B4_4678 at 5163.480 kb on - strand, within Pf1N1B4_4678 at 5163.716 kb on + strand, within Pf1N1B4_4678 at 5163.717 kb on - strand, within Pf1N1B4_4678 at 5163.896 kb on + strand, within Pf1N1B4_4678 at 5163.897 kb on - strand, within Pf1N1B4_4678 at 5163.995 kb on + strand, within Pf1N1B4_4678 at 5163.995 kb on + strand, within Pf1N1B4_4678 at 5163.995 kb on + strand, within Pf1N1B4_4678 at 5163.995 kb on + strand, within Pf1N1B4_4678 at 5163.996 kb on - strand at 5163.996 kb on - strand, within Pf1N1B4_4678 at 5163.996 kb on - strand at 5163.996 kb on - strand, within Pf1N1B4_4678 at 5164.296 kb on - strand at 5164.325 kb on + strand at 5164.607 kb on + strand at 5164.609 kb on + strand, within Pf1N1B4_4678 at 5164.646 kb on + strand, within Pf1N1B4_4678 at 5164.647 kb on - strand at 5164.709 kb on + strand at 5164.709 kb on + strand, within Pf1N1B4_4678 at 5164.710 kb on - strand at 5164.710 kb on - strand, within Pf1N1B4_4678 at 5164.932 kb on - strand, within Pf1N1B4_4678 at 5164.932 kb on - strand, within Pf1N1B4_4678 at 5165.222 kb on + strand at 5165.353 kb on + strand at 5165.354 kb on - strand at 5165.553 kb on + strand at 5165.554 kb on - strand at 5165.568 kb on + strand at 5165.568 kb on + strand at 5165.569 kb on - strand at 5165.569 kb on - strand at 5165.569 kb on - strand at 5165.569 kb on - strand at 5165.569 kb on - strand at 5165.686 kb on - strand, within Pf1N1B4_4679 at 5165.686 kb on - strand, within Pf1N1B4_4679 at 5165.686 kb on - strand, within Pf1N1B4_4679 at 5165.716 kb on - strand, within Pf1N1B4_4679 at 5165.745 kb on + strand, within Pf1N1B4_4679 at 5165.746 kb on - strand at 5165.823 kb on + strand at 5165.824 kb on - strand, within Pf1N1B4_4679 at 5165.839 kb on + strand, within Pf1N1B4_4679 at 5165.839 kb on + strand, within Pf1N1B4_4679 at 5165.840 kb on - strand, within Pf1N1B4_4679 at 5165.884 kb on + strand, within Pf1N1B4_4679 at 5165.885 kb on - strand, within Pf1N1B4_4679 at 5165.887 kb on + strand, within Pf1N1B4_4679 at 5166.011 kb on + strand, within Pf1N1B4_4679 at 5166.331 kb on + strand, within Pf1N1B4_4679 at 5166.347 kb on + strand, within Pf1N1B4_4679 at 5166.347 kb on + strand at 5166.348 kb on - strand, within Pf1N1B4_4679
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,162,323 - Pf1N1B4_4677 0.76 +0.6 5,162,755 - Pf1N1B4_4677 0.89 -1.5 5,162,756 + Pf1N1B4_4677 0.89 +0.5 5,162,756 + -0.5 5,162,756 + Pf1N1B4_4677 0.89 -0.6 5,162,756 + +0.5 5,162,756 + Pf1N1B4_4677 0.89 -0.9 5,162,756 + Pf1N1B4_4677 0.89 +0.5 5,162,757 - Pf1N1B4_4677 0.89 +0.9 5,162,757 - Pf1N1B4_4677 0.89 -0.8 5,162,911 - -0.5 5,162,927 + -0.1 5,162,927 + +1.5 5,163,064 + +0.1 5,163,067 - -1.1 5,163,114 + -0.5 5,163,124 + -3.7 5,163,124 + -1.3 5,163,124 + +0.5 5,163,444 - Pf1N1B4_4678 0.15 -2.1 5,163,480 - Pf1N1B4_4678 0.16 -0.5 5,163,716 + Pf1N1B4_4678 0.27 +0.5 5,163,717 - Pf1N1B4_4678 0.27 +0.1 5,163,896 + Pf1N1B4_4678 0.35 -1.3 5,163,897 - Pf1N1B4_4678 0.35 -3.3 5,163,995 + Pf1N1B4_4678 0.39 -1.8 5,163,995 + Pf1N1B4_4678 0.39 -1.7 5,163,995 + Pf1N1B4_4678 0.39 -2.1 5,163,995 + Pf1N1B4_4678 0.39 -2.5 5,163,996 - -1.5 5,163,996 - Pf1N1B4_4678 0.39 -0.5 5,163,996 - -0.5 5,163,996 - Pf1N1B4_4678 0.39 -1.5 5,164,296 - -0.5 5,164,325 + -0.5 5,164,607 + -0.5 5,164,609 + Pf1N1B4_4678 0.67 -0.5 5,164,646 + Pf1N1B4_4678 0.68 -2.1 5,164,647 - -0.5 5,164,709 + -1.5 5,164,709 + Pf1N1B4_4678 0.71 -2.8 5,164,710 - -0.5 5,164,710 - Pf1N1B4_4678 0.71 -1.1 5,164,932 - Pf1N1B4_4678 0.81 -2.5 5,164,932 - Pf1N1B4_4678 0.81 -2.5 5,165,222 + -1.3 5,165,353 + +0.5 5,165,354 - +1.5 5,165,553 + -1.5 5,165,554 - -0.2 5,165,568 + -0.5 5,165,568 + -2.8 5,165,569 - -1.1 5,165,569 - -2.5 5,165,569 - -0.5 5,165,569 - -0.0 5,165,569 - -1.5 5,165,686 - Pf1N1B4_4679 0.19 +2.1 5,165,686 - Pf1N1B4_4679 0.19 -2.1 5,165,686 - Pf1N1B4_4679 0.19 +0.1 5,165,716 - Pf1N1B4_4679 0.22 -1.0 5,165,745 + Pf1N1B4_4679 0.25 -1.5 5,165,746 - -1.1 5,165,823 + -0.5 5,165,824 - Pf1N1B4_4679 0.32 -0.5 5,165,839 + Pf1N1B4_4679 0.33 +0.2 5,165,839 + Pf1N1B4_4679 0.33 +1.1 5,165,840 - Pf1N1B4_4679 0.33 -0.8 5,165,884 + Pf1N1B4_4679 0.38 -0.9 5,165,885 - Pf1N1B4_4679 0.38 -0.5 5,165,887 + Pf1N1B4_4679 0.38 -0.1 5,166,011 + Pf1N1B4_4679 0.49 -0.5 5,166,331 + Pf1N1B4_4679 0.79 -0.7 5,166,347 + Pf1N1B4_4679 0.81 +0.5 5,166,347 + +0.1 5,166,348 - Pf1N1B4_4679 0.81 +0.3
Or see this region's nucleotide sequence