Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4678

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4677 and Pf1N1B4_4678 are separated by 2 nucleotidesPf1N1B4_4678 and Pf1N1B4_4679 are separated by 132 nucleotides Pf1N1B4_4677 - Trehalose synthase (EC 5.4.99.16), at 5,159,775 to 5,163,116 _4677 Pf1N1B4_4678 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 5,163,119 to 5,165,350 _4678 Pf1N1B4_4679 - outer membrane autotransporter, at 5,165,483 to 5,166,550 _4679 Position (kb) 5163 5164 5165 5166Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5162.323 kb on - strand, within Pf1N1B4_4677at 5162.755 kb on - strand, within Pf1N1B4_4677at 5162.756 kb on + strand, within Pf1N1B4_4677at 5162.756 kb on + strandat 5162.756 kb on + strand, within Pf1N1B4_4677at 5162.756 kb on + strandat 5162.756 kb on + strand, within Pf1N1B4_4677at 5162.756 kb on + strand, within Pf1N1B4_4677at 5162.757 kb on - strand, within Pf1N1B4_4677at 5162.757 kb on - strand, within Pf1N1B4_4677at 5162.911 kb on - strandat 5162.927 kb on + strandat 5162.927 kb on + strandat 5163.064 kb on + strandat 5163.067 kb on - strandat 5163.114 kb on + strandat 5163.124 kb on + strandat 5163.124 kb on + strandat 5163.124 kb on + strandat 5163.444 kb on - strand, within Pf1N1B4_4678at 5163.480 kb on - strand, within Pf1N1B4_4678at 5163.716 kb on + strand, within Pf1N1B4_4678at 5163.717 kb on - strand, within Pf1N1B4_4678at 5163.896 kb on + strand, within Pf1N1B4_4678at 5163.897 kb on - strand, within Pf1N1B4_4678at 5163.995 kb on + strand, within Pf1N1B4_4678at 5163.995 kb on + strand, within Pf1N1B4_4678at 5163.995 kb on + strand, within Pf1N1B4_4678at 5163.995 kb on + strand, within Pf1N1B4_4678at 5163.996 kb on - strandat 5163.996 kb on - strand, within Pf1N1B4_4678at 5163.996 kb on - strandat 5163.996 kb on - strand, within Pf1N1B4_4678at 5164.296 kb on - strandat 5164.325 kb on + strandat 5164.607 kb on + strandat 5164.609 kb on + strand, within Pf1N1B4_4678at 5164.646 kb on + strand, within Pf1N1B4_4678at 5164.647 kb on - strandat 5164.709 kb on + strandat 5164.709 kb on + strand, within Pf1N1B4_4678at 5164.710 kb on - strandat 5164.710 kb on - strand, within Pf1N1B4_4678at 5164.932 kb on - strand, within Pf1N1B4_4678at 5164.932 kb on - strand, within Pf1N1B4_4678at 5165.222 kb on + strandat 5165.353 kb on + strandat 5165.354 kb on - strandat 5165.553 kb on + strandat 5165.554 kb on - strandat 5165.568 kb on + strandat 5165.568 kb on + strandat 5165.569 kb on - strandat 5165.569 kb on - strandat 5165.569 kb on - strandat 5165.569 kb on - strandat 5165.569 kb on - strandat 5165.686 kb on - strand, within Pf1N1B4_4679at 5165.686 kb on - strand, within Pf1N1B4_4679at 5165.686 kb on - strand, within Pf1N1B4_4679at 5165.716 kb on - strand, within Pf1N1B4_4679at 5165.745 kb on + strand, within Pf1N1B4_4679at 5165.746 kb on - strandat 5165.823 kb on + strandat 5165.824 kb on - strand, within Pf1N1B4_4679at 5165.839 kb on + strand, within Pf1N1B4_4679at 5165.839 kb on + strand, within Pf1N1B4_4679at 5165.840 kb on - strand, within Pf1N1B4_4679at 5165.884 kb on + strand, within Pf1N1B4_4679at 5165.885 kb on - strand, within Pf1N1B4_4679at 5165.887 kb on + strand, within Pf1N1B4_4679at 5166.011 kb on + strand, within Pf1N1B4_4679at 5166.331 kb on + strand, within Pf1N1B4_4679at 5166.347 kb on + strand, within Pf1N1B4_4679at 5166.347 kb on + strandat 5166.348 kb on - strand, within Pf1N1B4_4679

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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5,162,323 - Pf1N1B4_4677 0.76 +0.6
5,162,755 - Pf1N1B4_4677 0.89 -1.5
5,162,756 + Pf1N1B4_4677 0.89 +0.5
5,162,756 + -0.5
5,162,756 + Pf1N1B4_4677 0.89 -0.6
5,162,756 + +0.5
5,162,756 + Pf1N1B4_4677 0.89 -0.9
5,162,756 + Pf1N1B4_4677 0.89 +0.5
5,162,757 - Pf1N1B4_4677 0.89 +0.9
5,162,757 - Pf1N1B4_4677 0.89 -0.8
5,162,911 - -0.5
5,162,927 + -0.1
5,162,927 + +1.5
5,163,064 + +0.1
5,163,067 - -1.1
5,163,114 + -0.5
5,163,124 + -3.7
5,163,124 + -1.3
5,163,124 + +0.5
5,163,444 - Pf1N1B4_4678 0.15 -2.1
5,163,480 - Pf1N1B4_4678 0.16 -0.5
5,163,716 + Pf1N1B4_4678 0.27 +0.5
5,163,717 - Pf1N1B4_4678 0.27 +0.1
5,163,896 + Pf1N1B4_4678 0.35 -1.3
5,163,897 - Pf1N1B4_4678 0.35 -3.3
5,163,995 + Pf1N1B4_4678 0.39 -1.8
5,163,995 + Pf1N1B4_4678 0.39 -1.7
5,163,995 + Pf1N1B4_4678 0.39 -2.1
5,163,995 + Pf1N1B4_4678 0.39 -2.5
5,163,996 - -1.5
5,163,996 - Pf1N1B4_4678 0.39 -0.5
5,163,996 - -0.5
5,163,996 - Pf1N1B4_4678 0.39 -1.5
5,164,296 - -0.5
5,164,325 + -0.5
5,164,607 + -0.5
5,164,609 + Pf1N1B4_4678 0.67 -0.5
5,164,646 + Pf1N1B4_4678 0.68 -2.1
5,164,647 - -0.5
5,164,709 + -1.5
5,164,709 + Pf1N1B4_4678 0.71 -2.8
5,164,710 - -0.5
5,164,710 - Pf1N1B4_4678 0.71 -1.1
5,164,932 - Pf1N1B4_4678 0.81 -2.5
5,164,932 - Pf1N1B4_4678 0.81 -2.5
5,165,222 + -1.3
5,165,353 + +0.5
5,165,354 - +1.5
5,165,553 + -1.5
5,165,554 - -0.2
5,165,568 + -0.5
5,165,568 + -2.8
5,165,569 - -1.1
5,165,569 - -2.5
5,165,569 - -0.5
5,165,569 - -0.0
5,165,569 - -1.5
5,165,686 - Pf1N1B4_4679 0.19 +2.1
5,165,686 - Pf1N1B4_4679 0.19 -2.1
5,165,686 - Pf1N1B4_4679 0.19 +0.1
5,165,716 - Pf1N1B4_4679 0.22 -1.0
5,165,745 + Pf1N1B4_4679 0.25 -1.5
5,165,746 - -1.1
5,165,823 + -0.5
5,165,824 - Pf1N1B4_4679 0.32 -0.5
5,165,839 + Pf1N1B4_4679 0.33 +0.2
5,165,839 + Pf1N1B4_4679 0.33 +1.1
5,165,840 - Pf1N1B4_4679 0.33 -0.8
5,165,884 + Pf1N1B4_4679 0.38 -0.9
5,165,885 - Pf1N1B4_4679 0.38 -0.5
5,165,887 + Pf1N1B4_4679 0.38 -0.1
5,166,011 + Pf1N1B4_4679 0.49 -0.5
5,166,331 + Pf1N1B4_4679 0.79 -0.7
5,166,347 + Pf1N1B4_4679 0.81 +0.5
5,166,347 + +0.1
5,166,348 - Pf1N1B4_4679 0.81 +0.3

Or see this region's nucleotide sequence