Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4649 and Pf1N1B4_4650 are separated by 159 nucleotides Pf1N1B4_4650 and Pf1N1B4_4651 are separated by 92 nucleotides Pf1N1B4_4651 and Pf1N1B4_4652 are separated by 70 nucleotides
Pf1N1B4_4649 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 5,128,882 to 5,129,391
_4649
Pf1N1B4_4650 - Aspartate racemase (EC 5.1.1.13), at 5,129,551 to 5,130,243
_4650
Pf1N1B4_4651 - Transcriptional regulator, AraC family, at 5,130,336 to 5,131,112
_4651
Pf1N1B4_4652 - FIG00958406: hypothetical protein, at 5,131,183 to 5,131,677
_4652
Position (kb)
5129
5130
5131 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5128.842 kb on - strand at 5128.955 kb on - strand at 5128.985 kb on + strand, within Pf1N1B4_4649 at 5129.330 kb on - strand, within Pf1N1B4_4649 at 5129.532 kb on + strand at 5129.762 kb on + strand, within Pf1N1B4_4650 at 5129.763 kb on - strand, within Pf1N1B4_4650 at 5129.763 kb on - strand, within Pf1N1B4_4650 at 5129.998 kb on - strand, within Pf1N1B4_4650 at 5130.122 kb on + strand, within Pf1N1B4_4650 at 5130.123 kb on - strand, within Pf1N1B4_4650 at 5130.123 kb on - strand, within Pf1N1B4_4650 at 5130.166 kb on + strand at 5130.166 kb on + strand, within Pf1N1B4_4650 at 5130.192 kb on - strand at 5130.192 kb on - strand at 5130.257 kb on - strand at 5130.321 kb on - strand at 5130.406 kb on - strand at 5130.528 kb on + strand at 5130.530 kb on + strand, within Pf1N1B4_4651 at 5130.530 kb on + strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand at 5130.607 kb on + strand, within Pf1N1B4_4651 at 5130.623 kb on - strand, within Pf1N1B4_4651 at 5130.689 kb on - strand, within Pf1N1B4_4651 at 5131.111 kb on - strand at 5131.222 kb on + strand at 5131.222 kb on + strand at 5131.222 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,128,842 - -0.5 5,128,955 - -1.2 5,128,985 + Pf1N1B4_4649 0.20 -1.5 5,129,330 - Pf1N1B4_4649 0.88 -0.0 5,129,532 + -2.1 5,129,762 + Pf1N1B4_4650 0.30 -1.5 5,129,763 - Pf1N1B4_4650 0.31 +0.1 5,129,763 - Pf1N1B4_4650 0.31 +0.3 5,129,998 - Pf1N1B4_4650 0.65 +1.5 5,130,122 + Pf1N1B4_4650 0.82 +1.3 5,130,123 - Pf1N1B4_4650 0.83 -1.8 5,130,123 - Pf1N1B4_4650 0.83 -0.5 5,130,166 + +2.1 5,130,166 + Pf1N1B4_4650 0.89 +0.1 5,130,192 - -2.5 5,130,192 - -0.5 5,130,257 - -0.5 5,130,321 - +0.5 5,130,406 - +0.5 5,130,528 + +0.5 5,130,530 + Pf1N1B4_4651 0.25 -0.5 5,130,530 + Pf1N1B4_4651 0.25 -1.0 5,130,531 - Pf1N1B4_4651 0.25 +1.1 5,130,531 - Pf1N1B4_4651 0.25 -0.1 5,130,531 - Pf1N1B4_4651 0.25 -0.5 5,130,531 - -0.5 5,130,607 + Pf1N1B4_4651 0.35 -0.3 5,130,623 - Pf1N1B4_4651 0.37 +0.0 5,130,689 - Pf1N1B4_4651 0.45 +0.9 5,131,111 - +0.5 5,131,222 + -0.8 5,131,222 + -0.5 5,131,222 + -1.1
Or see this region's nucleotide sequence