Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4650

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4649 and Pf1N1B4_4650 are separated by 159 nucleotidesPf1N1B4_4650 and Pf1N1B4_4651 are separated by 92 nucleotidesPf1N1B4_4651 and Pf1N1B4_4652 are separated by 70 nucleotides Pf1N1B4_4649 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 5,128,882 to 5,129,391 _4649 Pf1N1B4_4650 - Aspartate racemase (EC 5.1.1.13), at 5,129,551 to 5,130,243 _4650 Pf1N1B4_4651 - Transcriptional regulator, AraC family, at 5,130,336 to 5,131,112 _4651 Pf1N1B4_4652 - FIG00958406: hypothetical protein, at 5,131,183 to 5,131,677 _4652 Position (kb) 5129 5130 5131Strain fitness (log2 ratio) -2 -1 0 1 2at 5128.842 kb on - strandat 5128.955 kb on - strandat 5128.985 kb on + strand, within Pf1N1B4_4649at 5129.330 kb on - strand, within Pf1N1B4_4649at 5129.532 kb on + strandat 5129.762 kb on + strand, within Pf1N1B4_4650at 5129.763 kb on - strand, within Pf1N1B4_4650at 5129.763 kb on - strand, within Pf1N1B4_4650at 5129.998 kb on - strand, within Pf1N1B4_4650at 5130.122 kb on + strand, within Pf1N1B4_4650at 5130.123 kb on - strand, within Pf1N1B4_4650at 5130.123 kb on - strand, within Pf1N1B4_4650at 5130.166 kb on + strandat 5130.166 kb on + strand, within Pf1N1B4_4650at 5130.192 kb on - strandat 5130.192 kb on - strandat 5130.257 kb on - strandat 5130.321 kb on - strandat 5130.406 kb on - strandat 5130.528 kb on + strandat 5130.530 kb on + strand, within Pf1N1B4_4651at 5130.530 kb on + strand, within Pf1N1B4_4651at 5130.531 kb on - strand, within Pf1N1B4_4651at 5130.531 kb on - strand, within Pf1N1B4_4651at 5130.531 kb on - strand, within Pf1N1B4_4651at 5130.531 kb on - strandat 5130.607 kb on + strand, within Pf1N1B4_4651at 5130.623 kb on - strand, within Pf1N1B4_4651at 5130.689 kb on - strand, within Pf1N1B4_4651at 5131.111 kb on - strandat 5131.222 kb on + strandat 5131.222 kb on + strandat 5131.222 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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5,128,842 - -0.5
5,128,955 - -1.2
5,128,985 + Pf1N1B4_4649 0.20 -1.5
5,129,330 - Pf1N1B4_4649 0.88 -0.0
5,129,532 + -2.1
5,129,762 + Pf1N1B4_4650 0.30 -1.5
5,129,763 - Pf1N1B4_4650 0.31 +0.1
5,129,763 - Pf1N1B4_4650 0.31 +0.3
5,129,998 - Pf1N1B4_4650 0.65 +1.5
5,130,122 + Pf1N1B4_4650 0.82 +1.3
5,130,123 - Pf1N1B4_4650 0.83 -1.8
5,130,123 - Pf1N1B4_4650 0.83 -0.5
5,130,166 + +2.1
5,130,166 + Pf1N1B4_4650 0.89 +0.1
5,130,192 - -2.5
5,130,192 - -0.5
5,130,257 - -0.5
5,130,321 - +0.5
5,130,406 - +0.5
5,130,528 + +0.5
5,130,530 + Pf1N1B4_4651 0.25 -0.5
5,130,530 + Pf1N1B4_4651 0.25 -1.0
5,130,531 - Pf1N1B4_4651 0.25 +1.1
5,130,531 - Pf1N1B4_4651 0.25 -0.1
5,130,531 - Pf1N1B4_4651 0.25 -0.5
5,130,531 - -0.5
5,130,607 + Pf1N1B4_4651 0.35 -0.3
5,130,623 - Pf1N1B4_4651 0.37 +0.0
5,130,689 - Pf1N1B4_4651 0.45 +0.9
5,131,111 - +0.5
5,131,222 + -0.8
5,131,222 + -0.5
5,131,222 + -1.1

Or see this region's nucleotide sequence