Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4568

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4566 and Pf1N1B4_4567 are separated by 161 nucleotidesPf1N1B4_4567 and Pf1N1B4_4568 are separated by 107 nucleotidesPf1N1B4_4568 and Pf1N1B4_4569 are separated by 195 nucleotidesPf1N1B4_4569 and Pf1N1B4_4570 are separated by 35 nucleotides Pf1N1B4_4566 - Universal stress protein family 7, at 5,039,576 to 5,040,439 _4566 Pf1N1B4_4567 - tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-), at 5,040,601 to 5,041,209 _4567 Pf1N1B4_4568 - FIG00793915: hypothetical protein, at 5,041,317 to 5,041,805 _4568 Pf1N1B4_4569 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 5,042,001 to 5,042,618 _4569 Pf1N1B4_4570 - Probable carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23), at 5,042,654 to 5,043,505 _4570 Position (kb) 5041 5042Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5040.483 kb on + strandat 5040.483 kb on + strandat 5040.483 kb on + strandat 5040.483 kb on + strandat 5040.483 kb on + strandat 5040.483 kb on + strandat 5040.483 kb on + strandat 5040.484 kb on - strandat 5040.484 kb on - strandat 5040.484 kb on - strandat 5040.485 kb on + strandat 5040.485 kb on + strandat 5040.485 kb on + strandat 5040.485 kb on + strandat 5040.486 kb on - strandat 5040.486 kb on - strandat 5040.486 kb on - strandat 5040.486 kb on - strandat 5040.580 kb on + strandat 5040.751 kb on + strand, within Pf1N1B4_4567at 5040.752 kb on - strand, within Pf1N1B4_4567at 5041.027 kb on + strand, within Pf1N1B4_4567at 5041.028 kb on - strand, within Pf1N1B4_4567at 5041.090 kb on + strand, within Pf1N1B4_4567at 5041.091 kb on - strand, within Pf1N1B4_4567at 5041.215 kb on + strandat 5041.216 kb on - strandat 5041.319 kb on - strandat 5041.530 kb on + strandat 5041.618 kb on + strandat 5041.618 kb on + strandat 5041.619 kb on - strand, within Pf1N1B4_4568at 5041.619 kb on - strand, within Pf1N1B4_4568at 5041.741 kb on + strand, within Pf1N1B4_4568at 5041.960 kb on - strandat 5042.026 kb on + strandat 5042.027 kb on - strandat 5042.084 kb on + strand, within Pf1N1B4_4569at 5042.084 kb on + strand, within Pf1N1B4_4569at 5042.179 kb on + strand, within Pf1N1B4_4569at 5042.180 kb on - strand, within Pf1N1B4_4569at 5042.501 kb on - strand, within Pf1N1B4_4569at 5042.736 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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5,040,483 + +1.8
5,040,483 + +0.5
5,040,483 + +0.5
5,040,483 + +0.3
5,040,483 + +0.1
5,040,483 + -0.9
5,040,483 + +0.5
5,040,484 - -1.0
5,040,484 - -0.3
5,040,484 - +0.2
5,040,485 + -0.9
5,040,485 + -2.8
5,040,485 + +1.1
5,040,485 + +0.5
5,040,486 - -1.1
5,040,486 - +1.8
5,040,486 - -1.1
5,040,486 - +1.5
5,040,580 + +0.5
5,040,751 + Pf1N1B4_4567 0.25 +1.2
5,040,752 - Pf1N1B4_4567 0.25 -1.2
5,041,027 + Pf1N1B4_4567 0.70 -0.9
5,041,028 - Pf1N1B4_4567 0.70 -2.5
5,041,090 + Pf1N1B4_4567 0.80 +0.0
5,041,091 - Pf1N1B4_4567 0.80 +0.2
5,041,215 + +0.2
5,041,216 - -0.7
5,041,319 - +1.1
5,041,530 + +0.1
5,041,618 + -1.5
5,041,618 + +0.1
5,041,619 - Pf1N1B4_4568 0.62 +0.6
5,041,619 - Pf1N1B4_4568 0.62 -0.1
5,041,741 + Pf1N1B4_4568 0.87 -0.1
5,041,960 - -0.3
5,042,026 + -0.5
5,042,027 - -1.1
5,042,084 + Pf1N1B4_4569 0.13 -0.5
5,042,084 + Pf1N1B4_4569 0.13 -1.1
5,042,179 + Pf1N1B4_4569 0.29 -1.5
5,042,180 - Pf1N1B4_4569 0.29 -0.2
5,042,501 - Pf1N1B4_4569 0.81 -0.1
5,042,736 + -0.8

Or see this region's nucleotide sequence