Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4566 and Pf1N1B4_4567 are separated by 161 nucleotides Pf1N1B4_4567 and Pf1N1B4_4568 are separated by 107 nucleotides Pf1N1B4_4568 and Pf1N1B4_4569 are separated by 195 nucleotides Pf1N1B4_4569 and Pf1N1B4_4570 are separated by 35 nucleotides
Pf1N1B4_4566 - Universal stress protein family 7, at 5,039,576 to 5,040,439
_4566
Pf1N1B4_4567 - tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-), at 5,040,601 to 5,041,209
_4567
Pf1N1B4_4568 - FIG00793915: hypothetical protein, at 5,041,317 to 5,041,805
_4568
Pf1N1B4_4569 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 5,042,001 to 5,042,618
_4569
Pf1N1B4_4570 - Probable carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23), at 5,042,654 to 5,043,505
_4570
Position (kb)
5041
5042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.483 kb on + strand at 5040.484 kb on - strand at 5040.484 kb on - strand at 5040.484 kb on - strand at 5040.485 kb on + strand at 5040.485 kb on + strand at 5040.485 kb on + strand at 5040.485 kb on + strand at 5040.486 kb on - strand at 5040.486 kb on - strand at 5040.486 kb on - strand at 5040.486 kb on - strand at 5040.580 kb on + strand at 5040.751 kb on + strand, within Pf1N1B4_4567 at 5040.752 kb on - strand, within Pf1N1B4_4567 at 5041.027 kb on + strand, within Pf1N1B4_4567 at 5041.028 kb on - strand, within Pf1N1B4_4567 at 5041.090 kb on + strand, within Pf1N1B4_4567 at 5041.091 kb on - strand, within Pf1N1B4_4567 at 5041.215 kb on + strand at 5041.216 kb on - strand at 5041.319 kb on - strand at 5041.530 kb on + strand at 5041.618 kb on + strand at 5041.618 kb on + strand at 5041.619 kb on - strand, within Pf1N1B4_4568 at 5041.619 kb on - strand, within Pf1N1B4_4568 at 5041.741 kb on + strand, within Pf1N1B4_4568 at 5041.960 kb on - strand at 5042.026 kb on + strand at 5042.027 kb on - strand at 5042.084 kb on + strand, within Pf1N1B4_4569 at 5042.084 kb on + strand, within Pf1N1B4_4569 at 5042.179 kb on + strand, within Pf1N1B4_4569 at 5042.180 kb on - strand, within Pf1N1B4_4569 at 5042.501 kb on - strand, within Pf1N1B4_4569 at 5042.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,040,483 + +1.8 5,040,483 + +0.5 5,040,483 + +0.5 5,040,483 + +0.3 5,040,483 + +0.1 5,040,483 + -0.9 5,040,483 + +0.5 5,040,484 - -1.0 5,040,484 - -0.3 5,040,484 - +0.2 5,040,485 + -0.9 5,040,485 + -2.8 5,040,485 + +1.1 5,040,485 + +0.5 5,040,486 - -1.1 5,040,486 - +1.8 5,040,486 - -1.1 5,040,486 - +1.5 5,040,580 + +0.5 5,040,751 + Pf1N1B4_4567 0.25 +1.2 5,040,752 - Pf1N1B4_4567 0.25 -1.2 5,041,027 + Pf1N1B4_4567 0.70 -0.9 5,041,028 - Pf1N1B4_4567 0.70 -2.5 5,041,090 + Pf1N1B4_4567 0.80 +0.0 5,041,091 - Pf1N1B4_4567 0.80 +0.2 5,041,215 + +0.2 5,041,216 - -0.7 5,041,319 - +1.1 5,041,530 + +0.1 5,041,618 + -1.5 5,041,618 + +0.1 5,041,619 - Pf1N1B4_4568 0.62 +0.6 5,041,619 - Pf1N1B4_4568 0.62 -0.1 5,041,741 + Pf1N1B4_4568 0.87 -0.1 5,041,960 - -0.3 5,042,026 + -0.5 5,042,027 - -1.1 5,042,084 + Pf1N1B4_4569 0.13 -0.5 5,042,084 + Pf1N1B4_4569 0.13 -1.1 5,042,179 + Pf1N1B4_4569 0.29 -1.5 5,042,180 - Pf1N1B4_4569 0.29 -0.2 5,042,501 - Pf1N1B4_4569 0.81 -0.1 5,042,736 + -0.8
Or see this region's nucleotide sequence