Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4532

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4532 and Pf1N1B4_4533 are separated by 2 nucleotides Pf1N1B4_4532 - ClpB protein, at 4,997,929 to 5,000,472 _4532 Pf1N1B4_4533 - Uncharacterized protein ImpH/VasB, at 5,000,475 to 5,001,500 _4533 Pf1N1B4_4534 - Protein ImpG/VasA, at 5,001,464 to 5,003,257 _4534 Position (kb) 4997 4998 4999 5000 5001Strain fitness (log2 ratio) -2 -1 0 1 2at 4996.986 kb on - strandat 4997.210 kb on + strandat 4997.370 kb on - strandat 4997.408 kb on + strandat 4997.453 kb on - strandat 4997.740 kb on + strandat 4997.920 kb on - strandat 4998.200 kb on - strandat 4998.205 kb on - strand, within Pf1N1B4_4532at 4998.362 kb on + strand, within Pf1N1B4_4532at 4998.363 kb on - strand, within Pf1N1B4_4532at 4998.674 kb on + strand, within Pf1N1B4_4532at 4999.290 kb on - strand, within Pf1N1B4_4532at 5000.138 kb on - strand, within Pf1N1B4_4532at 5000.318 kb on + strandat 5000.319 kb on - strandat 5000.378 kb on + strandat 5000.379 kb on - strandat 5000.867 kb on - strand, within Pf1N1B4_4533at 5001.107 kb on - strand, within Pf1N1B4_4533at 5001.107 kb on - strand, within Pf1N1B4_4533

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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4,996,986 - +0.6
4,997,210 + +1.6
4,997,370 - -1.2
4,997,408 + +0.6
4,997,453 - +0.4
4,997,740 + +0.1
4,997,920 - +0.1
4,998,200 - -0.4
4,998,205 - Pf1N1B4_4532 0.11 +0.6
4,998,362 + Pf1N1B4_4532 0.17 +0.3
4,998,363 - Pf1N1B4_4532 0.17 +1.1
4,998,674 + Pf1N1B4_4532 0.29 -2.3
4,999,290 - Pf1N1B4_4532 0.53 +1.9
5,000,138 - Pf1N1B4_4532 0.87 -0.4
5,000,318 + -0.4
5,000,319 - -0.4
5,000,378 + -1.0
5,000,379 - +1.3
5,000,867 - Pf1N1B4_4533 0.38 -0.9
5,001,107 - Pf1N1B4_4533 0.62 -0.4
5,001,107 - Pf1N1B4_4533 0.62 -0.9

Or see this region's nucleotide sequence