Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4519 and Pf1N1B4_4520 are separated by 40 nucleotides Pf1N1B4_4520 and Pf1N1B4_4521 are separated by 2 nucleotides Pf1N1B4_4521 and Pf1N1B4_4522 are separated by 140 nucleotides Pf1N1B4_4522 and Pf1N1B4_4523 are separated by 3 nucleotides
Pf1N1B4_4519 - Beta-ureidopropionase (EC 3.5.1.6), at 4,983,311 to 4,984,594
_4519
Pf1N1B4_4520 - GENE II AND X PROTEINS, at 4,984,635 to 4,985,048
_4520
Pf1N1B4_4521 - Probable transmembrane protein, at 4,985,051 to 4,985,485
_4521
Pf1N1B4_4522 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 4,985,626 to 4,986,060
_4522
Pf1N1B4_4523 - RNA polymerase sigma-70 factor, ECF subfamily, at 4,986,064 to 4,986,957
_4523
Position (kb)
4985
4986 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4984.146 kb on - strand, within Pf1N1B4_4519 at 4985.265 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.300 kb on + strand, within Pf1N1B4_4521 at 4985.301 kb on - strand, within Pf1N1B4_4521 at 4985.518 kb on - strand at 4985.966 kb on + strand, within Pf1N1B4_4522 at 4985.966 kb on + strand, within Pf1N1B4_4522 at 4986.285 kb on + strand, within Pf1N1B4_4523 at 4986.285 kb on + strand, within Pf1N1B4_4523 at 4986.301 kb on + strand, within Pf1N1B4_4523
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,984,146 - Pf1N1B4_4519 0.65 -0.4 4,985,265 - Pf1N1B4_4521 0.49 +1.9 4,985,269 - -0.4 4,985,269 - Pf1N1B4_4521 0.50 -0.0 4,985,269 - Pf1N1B4_4521 0.50 +0.3 4,985,269 - Pf1N1B4_4521 0.50 +1.1 4,985,300 + Pf1N1B4_4521 0.57 +1.1 4,985,301 - Pf1N1B4_4521 0.57 +0.6 4,985,518 - -0.4 4,985,966 + Pf1N1B4_4522 0.78 -0.4 4,985,966 + Pf1N1B4_4522 0.78 -0.4 4,986,285 + Pf1N1B4_4523 0.25 -2.0 4,986,285 + Pf1N1B4_4523 0.25 -1.4 4,986,301 + Pf1N1B4_4523 0.27 +0.1
Or see this region's nucleotide sequence