Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4481

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4480 and Pf1N1B4_4481 are separated by 185 nucleotidesPf1N1B4_4481 and Pf1N1B4_4482 are separated by 128 nucleotides Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746 _4480 Pf1N1B4_4481 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 4,942,932 to 4,943,420 _4481 Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412 _4482 Position (kb) 4942 4943 4944Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 4941.970 kb on - strand, within Pf1N1B4_4480at 4942.235 kb on + strand, within Pf1N1B4_4480at 4942.427 kb on + strand, within Pf1N1B4_4480at 4942.668 kb on - strandat 4942.753 kb on + strandat 4942.753 kb on + strandat 4942.753 kb on + strandat 4942.779 kb on - strandat 4942.779 kb on - strandat 4942.851 kb on - strandat 4942.904 kb on - strandat 4943.226 kb on + strand, within Pf1N1B4_4481at 4943.251 kb on - strand, within Pf1N1B4_4481at 4943.251 kb on - strandat 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.550 kb on + strandat 4943.550 kb on + strandat 4943.578 kb on - strandat 4943.809 kb on - strand, within Pf1N1B4_4482at 4943.809 kb on - strand, within Pf1N1B4_4482at 4943.809 kb on - strand, within Pf1N1B4_4482at 4943.809 kb on - strand, within Pf1N1B4_4482at 4943.958 kb on + strand, within Pf1N1B4_4482at 4943.959 kb on - strand, within Pf1N1B4_4482at 4943.959 kb on - strand, within Pf1N1B4_4482at 4943.959 kb on - strand, within Pf1N1B4_4482at 4943.978 kb on - strand, within Pf1N1B4_4482at 4944.303 kb on + strand, within Pf1N1B4_4482at 4944.351 kb on + strandat 4944.352 kb on - strandat 4944.360 kb on + strandat 4944.360 kb on + strandat 4944.360 kb on + strandat 4944.360 kb on + strandat 4944.361 kb on - strandat 4944.366 kb on + strandat 4944.367 kb on - strandat 4944.367 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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4,941,970 - Pf1N1B4_4480 0.37 -0.8
4,942,235 + Pf1N1B4_4480 0.59 -1.0
4,942,427 + Pf1N1B4_4480 0.74 -4.8
4,942,668 - -2.0
4,942,753 + -2.4
4,942,753 + -2.8
4,942,753 + -3.0
4,942,779 - -0.4
4,942,779 - -0.4
4,942,851 - -0.4
4,942,904 - -1.4
4,943,226 + Pf1N1B4_4481 0.60 -3.0
4,943,251 - Pf1N1B4_4481 0.65 -3.0
4,943,251 - -0.4
4,943,370 + Pf1N1B4_4481 0.90 -2.8
4,943,370 + Pf1N1B4_4481 0.90 -3.2
4,943,370 + Pf1N1B4_4481 0.90 -1.4
4,943,372 + -1.8
4,943,372 + -0.4
4,943,372 + -2.0
4,943,372 + -1.4
4,943,550 + -0.0
4,943,550 + -1.0
4,943,578 - +1.0
4,943,809 - Pf1N1B4_4482 0.30 +1.2
4,943,809 - Pf1N1B4_4482 0.30 +0.6
4,943,809 - Pf1N1B4_4482 0.30 -2.0
4,943,809 - Pf1N1B4_4482 0.30 -0.4
4,943,958 + Pf1N1B4_4482 0.47 -0.0
4,943,959 - Pf1N1B4_4482 0.47 -1.0
4,943,959 - Pf1N1B4_4482 0.47 -1.4
4,943,959 - Pf1N1B4_4482 0.47 +2.7
4,943,978 - Pf1N1B4_4482 0.50 -1.0
4,944,303 + Pf1N1B4_4482 0.87 -0.0
4,944,351 + +1.6
4,944,352 - -1.2
4,944,360 + +0.8
4,944,360 + -3.0
4,944,360 + -0.4
4,944,360 + -0.4
4,944,361 - +0.6
4,944,366 + +1.4
4,944,367 - +0.1
4,944,367 - -1.4

Or see this region's nucleotide sequence