Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4480 and Pf1N1B4_4481 are separated by 185 nucleotides Pf1N1B4_4481 and Pf1N1B4_4482 are separated by 128 nucleotides
Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746
_4480
Pf1N1B4_4481 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 4,942,932 to 4,943,420
_4481
Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412
_4482
Position (kb)
4942
4943
4944 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4941.970 kb on - strand, within Pf1N1B4_4480 at 4942.235 kb on + strand, within Pf1N1B4_4480 at 4942.427 kb on + strand, within Pf1N1B4_4480 at 4942.668 kb on - strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.779 kb on - strand at 4942.779 kb on - strand at 4942.851 kb on - strand at 4942.904 kb on - strand at 4943.226 kb on + strand, within Pf1N1B4_4481 at 4943.251 kb on - strand, within Pf1N1B4_4481 at 4943.251 kb on - strand at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.550 kb on + strand at 4943.550 kb on + strand at 4943.578 kb on - strand at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.958 kb on + strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.978 kb on - strand, within Pf1N1B4_4482 at 4944.303 kb on + strand, within Pf1N1B4_4482 at 4944.351 kb on + strand at 4944.352 kb on - strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.361 kb on - strand at 4944.366 kb on + strand at 4944.367 kb on - strand at 4944.367 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,941,970 - Pf1N1B4_4480 0.37 -0.8 4,942,235 + Pf1N1B4_4480 0.59 -1.0 4,942,427 + Pf1N1B4_4480 0.74 -4.8 4,942,668 - -2.0 4,942,753 + -2.4 4,942,753 + -2.8 4,942,753 + -3.0 4,942,779 - -0.4 4,942,779 - -0.4 4,942,851 - -0.4 4,942,904 - -1.4 4,943,226 + Pf1N1B4_4481 0.60 -3.0 4,943,251 - Pf1N1B4_4481 0.65 -3.0 4,943,251 - -0.4 4,943,370 + Pf1N1B4_4481 0.90 -2.8 4,943,370 + Pf1N1B4_4481 0.90 -3.2 4,943,370 + Pf1N1B4_4481 0.90 -1.4 4,943,372 + -1.8 4,943,372 + -0.4 4,943,372 + -2.0 4,943,372 + -1.4 4,943,550 + -0.0 4,943,550 + -1.0 4,943,578 - +1.0 4,943,809 - Pf1N1B4_4482 0.30 +1.2 4,943,809 - Pf1N1B4_4482 0.30 +0.6 4,943,809 - Pf1N1B4_4482 0.30 -2.0 4,943,809 - Pf1N1B4_4482 0.30 -0.4 4,943,958 + Pf1N1B4_4482 0.47 -0.0 4,943,959 - Pf1N1B4_4482 0.47 -1.0 4,943,959 - Pf1N1B4_4482 0.47 -1.4 4,943,959 - Pf1N1B4_4482 0.47 +2.7 4,943,978 - Pf1N1B4_4482 0.50 -1.0 4,944,303 + Pf1N1B4_4482 0.87 -0.0 4,944,351 + +1.6 4,944,352 - -1.2 4,944,360 + +0.8 4,944,360 + -3.0 4,944,360 + -0.4 4,944,360 + -0.4 4,944,361 - +0.6 4,944,366 + +1.4 4,944,367 - +0.1 4,944,367 - -1.4
Or see this region's nucleotide sequence