Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4477 and Pf1N1B4_4478 are separated by 18 nucleotides Pf1N1B4_4478 and Pf1N1B4_4479 are separated by 0 nucleotides
Pf1N1B4_4477 - Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4), at 4,937,778 to 4,939,160
_4477
Pf1N1B4_4478 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 4,939,179 to 4,940,450
_4478
Pf1N1B4_4479 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 4,940,451 to 4,941,509
_4479
Position (kb)
4939
4940
4941 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4938.254 kb on - strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.432 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.619 kb on + strand, within Pf1N1B4_4477 at 4939.108 kb on + strand at 4939.181 kb on - strand at 4939.787 kb on - strand at 4939.933 kb on + strand, within Pf1N1B4_4478 at 4939.934 kb on - strand, within Pf1N1B4_4478 at 4939.934 kb on - strand, within Pf1N1B4_4478 at 4940.195 kb on + strand, within Pf1N1B4_4478 at 4940.432 kb on + strand at 4940.432 kb on + strand at 4940.433 kb on - strand at 4940.506 kb on + strand at 4940.575 kb on + strand, within Pf1N1B4_4479 at 4940.773 kb on + strand, within Pf1N1B4_4479 at 4940.774 kb on - strand at 4940.774 kb on - strand, within Pf1N1B4_4479 at 4940.774 kb on - strand, within Pf1N1B4_4479 at 4940.788 kb on + strand, within Pf1N1B4_4479 at 4940.789 kb on - strand, within Pf1N1B4_4479 at 4940.851 kb on + strand, within Pf1N1B4_4479 at 4940.852 kb on - strand, within Pf1N1B4_4479 at 4940.852 kb on - strand, within Pf1N1B4_4479 at 4940.935 kb on + strand, within Pf1N1B4_4479 at 4940.935 kb on + strand, within Pf1N1B4_4479 at 4941.010 kb on + strand, within Pf1N1B4_4479 at 4941.095 kb on - strand, within Pf1N1B4_4479 at 4941.209 kb on - strand, within Pf1N1B4_4479 at 4941.323 kb on - strand, within Pf1N1B4_4479 at 4941.367 kb on + strand, within Pf1N1B4_4479
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,938,254 - Pf1N1B4_4477 0.34 -2.0 4,938,431 + Pf1N1B4_4477 0.47 -2.8 4,938,431 + Pf1N1B4_4477 0.47 -3.2 4,938,432 - Pf1N1B4_4477 0.47 -2.0 4,938,533 - Pf1N1B4_4477 0.55 -0.4 4,938,533 - Pf1N1B4_4477 0.55 -1.4 4,938,619 + Pf1N1B4_4477 0.61 -2.8 4,939,108 + -2.0 4,939,181 - -2.0 4,939,787 - -2.0 4,939,933 + Pf1N1B4_4478 0.59 -3.9 4,939,934 - Pf1N1B4_4478 0.59 -0.4 4,939,934 - Pf1N1B4_4478 0.59 -3.7 4,940,195 + Pf1N1B4_4478 0.80 -1.4 4,940,432 + -0.4 4,940,432 + -2.0 4,940,433 - -0.4 4,940,506 + +1.2 4,940,575 + Pf1N1B4_4479 0.12 -0.4 4,940,773 + Pf1N1B4_4479 0.30 -0.4 4,940,774 - -1.4 4,940,774 - Pf1N1B4_4479 0.31 -0.4 4,940,774 - Pf1N1B4_4479 0.31 -2.4 4,940,788 + Pf1N1B4_4479 0.32 -2.4 4,940,789 - Pf1N1B4_4479 0.32 +1.6 4,940,851 + Pf1N1B4_4479 0.38 -3.8 4,940,852 - Pf1N1B4_4479 0.38 -2.2 4,940,852 - Pf1N1B4_4479 0.38 -0.4 4,940,935 + Pf1N1B4_4479 0.46 -0.4 4,940,935 + Pf1N1B4_4479 0.46 -2.4 4,941,010 + Pf1N1B4_4479 0.53 -2.0 4,941,095 - Pf1N1B4_4479 0.61 -0.4 4,941,209 - Pf1N1B4_4479 0.72 -1.4 4,941,323 - Pf1N1B4_4479 0.82 -1.4 4,941,367 + Pf1N1B4_4479 0.86 -0.4
Or see this region's nucleotide sequence