Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4476 and Pf1N1B4_4477 are separated by 234 nucleotides Pf1N1B4_4477 and Pf1N1B4_4478 are separated by 18 nucleotides
Pf1N1B4_4476 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 4,936,524 to 4,937,543
_4476
Pf1N1B4_4477 - Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4), at 4,937,778 to 4,939,160
_4477
Pf1N1B4_4478 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 4,939,179 to 4,940,450
_4478
Position (kb)
4937
4938
4939
4940 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4936.918 kb on + strand, within Pf1N1B4_4476 at 4936.919 kb on - strand, within Pf1N1B4_4476 at 4936.978 kb on + strand, within Pf1N1B4_4476 at 4936.978 kb on + strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4937.387 kb on - strand, within Pf1N1B4_4476 at 4937.500 kb on + strand at 4937.501 kb on - strand at 4937.554 kb on - strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.780 kb on - strand at 4938.254 kb on - strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.432 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.619 kb on + strand, within Pf1N1B4_4477 at 4939.108 kb on + strand at 4939.181 kb on - strand at 4939.787 kb on - strand at 4939.933 kb on + strand, within Pf1N1B4_4478 at 4939.934 kb on - strand, within Pf1N1B4_4478 at 4939.934 kb on - strand, within Pf1N1B4_4478
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,936,918 + Pf1N1B4_4476 0.39 -2.4 4,936,919 - Pf1N1B4_4476 0.39 -3.0 4,936,978 + Pf1N1B4_4476 0.45 -1.4 4,936,978 + Pf1N1B4_4476 0.45 -3.2 4,936,979 - Pf1N1B4_4476 0.45 -1.0 4,936,979 - Pf1N1B4_4476 0.45 -0.4 4,936,979 - Pf1N1B4_4476 0.45 -2.0 4,937,387 - Pf1N1B4_4476 0.85 -1.4 4,937,500 + -1.4 4,937,501 - -1.4 4,937,554 - -1.4 4,937,616 + +1.1 4,937,616 + -1.4 4,937,616 + -2.0 4,937,616 + -2.0 4,937,616 + -2.8 4,937,616 + -2.0 4,937,617 - +1.1 4,937,617 - -2.4 4,937,617 - +0.6 4,937,617 - +0.8 4,937,617 - -1.4 4,937,617 - +0.1 4,937,617 - -1.0 4,937,780 - -3.4 4,938,254 - Pf1N1B4_4477 0.34 -2.0 4,938,431 + Pf1N1B4_4477 0.47 -2.8 4,938,431 + Pf1N1B4_4477 0.47 -3.2 4,938,432 - Pf1N1B4_4477 0.47 -2.0 4,938,533 - Pf1N1B4_4477 0.55 -0.4 4,938,533 - Pf1N1B4_4477 0.55 -1.4 4,938,619 + Pf1N1B4_4477 0.61 -2.8 4,939,108 + -2.0 4,939,181 - -2.0 4,939,787 - -2.0 4,939,933 + Pf1N1B4_4478 0.59 -3.9 4,939,934 - Pf1N1B4_4478 0.59 -0.4 4,939,934 - Pf1N1B4_4478 0.59 -3.7
Or see this region's nucleotide sequence