Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4401 and Pf1N1B4_4402 are separated by 248 nucleotides Pf1N1B4_4402 and Pf1N1B4_4403 are separated by 122 nucleotides
Pf1N1B4_4401 - Non-heme chloroperoxidase (EC 1.11.1.10), at 4,860,491 to 4,861,321
_4401
Pf1N1B4_4402 - SoxH protein, homolog, at 4,861,570 to 4,862,466
_4402
Pf1N1B4_4403 - NADH dehydrogenase (EC 1.6.99.3), at 4,862,589 to 4,863,839
_4403
Position (kb)
4861
4862
4863 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4860.596 kb on + strand, within Pf1N1B4_4401 at 4860.596 kb on + strand, within Pf1N1B4_4401 at 4860.597 kb on - strand at 4860.733 kb on - strand at 4860.840 kb on - strand, within Pf1N1B4_4401 at 4860.965 kb on + strand, within Pf1N1B4_4401 at 4861.098 kb on + strand, within Pf1N1B4_4401 at 4861.098 kb on + strand, within Pf1N1B4_4401 at 4861.099 kb on - strand, within Pf1N1B4_4401 at 4861.099 kb on - strand, within Pf1N1B4_4401 at 4861.309 kb on + strand at 4861.309 kb on + strand at 4861.313 kb on - strand at 4861.360 kb on + strand at 4861.360 kb on + strand at 4861.360 kb on + strand at 4861.361 kb on - strand at 4861.361 kb on - strand at 4861.469 kb on + strand at 4861.469 kb on + strand at 4861.470 kb on - strand at 4861.470 kb on - strand at 4861.470 kb on - strand at 4861.470 kb on - strand at 4861.470 kb on - strand at 4861.470 kb on - strand at 4861.470 kb on - strand at 4861.575 kb on - strand at 4861.824 kb on - strand, within Pf1N1B4_4402 at 4861.824 kb on - strand, within Pf1N1B4_4402 at 4861.889 kb on + strand, within Pf1N1B4_4402 at 4861.890 kb on - strand at 4861.922 kb on + strand, within Pf1N1B4_4402 at 4862.260 kb on + strand, within Pf1N1B4_4402 at 4862.550 kb on + strand at 4862.551 kb on - strand at 4862.551 kb on - strand at 4862.777 kb on - strand, within Pf1N1B4_4403 at 4862.933 kb on - strand, within Pf1N1B4_4403 at 4863.149 kb on - strand, within Pf1N1B4_4403
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,860,596 + Pf1N1B4_4401 0.13 +1.5 4,860,596 + Pf1N1B4_4401 0.13 +1.2 4,860,597 - +0.1 4,860,733 - +0.5 4,860,840 - Pf1N1B4_4401 0.42 -0.5 4,860,965 + Pf1N1B4_4401 0.57 +0.1 4,861,098 + Pf1N1B4_4401 0.73 +0.1 4,861,098 + Pf1N1B4_4401 0.73 -0.9 4,861,099 - Pf1N1B4_4401 0.73 +0.7 4,861,099 - Pf1N1B4_4401 0.73 -1.5 4,861,309 + +2.1 4,861,309 + +0.5 4,861,313 - -1.8 4,861,360 + +0.8 4,861,360 + +1.5 4,861,360 + -1.1 4,861,361 - -0.1 4,861,361 - +0.0 4,861,469 + +0.5 4,861,469 + +0.5 4,861,470 - -0.1 4,861,470 - -0.1 4,861,470 - +1.1 4,861,470 - -3.5 4,861,470 - -0.9 4,861,470 - -0.1 4,861,470 - -2.5 4,861,575 - -0.5 4,861,824 - Pf1N1B4_4402 0.28 +0.8 4,861,824 - Pf1N1B4_4402 0.28 +1.5 4,861,889 + Pf1N1B4_4402 0.36 +0.5 4,861,890 - +0.1 4,861,922 + Pf1N1B4_4402 0.39 +0.5 4,862,260 + Pf1N1B4_4402 0.77 -0.4 4,862,550 + +1.1 4,862,551 - +1.1 4,862,551 - -1.5 4,862,777 - Pf1N1B4_4403 0.15 -0.8 4,862,933 - Pf1N1B4_4403 0.27 -1.1 4,863,149 - Pf1N1B4_4403 0.45 +0.5
Or see this region's nucleotide sequence