Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4366 and Pf1N1B4_4367 are separated by 632 nucleotides Pf1N1B4_4367 and Pf1N1B4_4368 are separated by 15 nucleotides Pf1N1B4_4368 and Pf1N1B4_4369 are separated by 155 nucleotides
Pf1N1B4_4366 - hypothetical protein, at 4,824,247 to 4,824,504
_4366
Pf1N1B4_4367 - hypothetical protein, at 4,825,137 to 4,825,487
_4367
Pf1N1B4_4368 - Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 4,825,503 to 4,825,925
_4368
Pf1N1B4_4369 - hypothetical protein, at 4,826,081 to 4,827,409
_4369
Position (kb)
4825
4826 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4824.608 kb on + strand at 4824.608 kb on + strand at 4824.608 kb on + strand at 4824.609 kb on - strand at 4824.679 kb on + strand at 4824.680 kb on - strand at 4824.715 kb on + strand at 4824.715 kb on + strand at 4824.716 kb on - strand at 4824.716 kb on - strand at 4824.737 kb on - strand at 4824.737 kb on - strand at 4824.744 kb on - strand at 4824.792 kb on + strand at 4824.793 kb on - strand at 4824.871 kb on + strand at 4824.871 kb on + strand at 4824.871 kb on + strand at 4824.977 kb on + strand at 4824.978 kb on - strand at 4824.978 kb on - strand at 4824.978 kb on - strand at 4824.978 kb on - strand at 4825.063 kb on + strand at 4825.197 kb on + strand, within Pf1N1B4_4367 at 4825.197 kb on + strand, within Pf1N1B4_4367 at 4825.581 kb on + strand, within Pf1N1B4_4368 at 4825.581 kb on + strand, within Pf1N1B4_4368 at 4825.583 kb on + strand, within Pf1N1B4_4368 at 4825.583 kb on + strand at 4825.583 kb on + strand, within Pf1N1B4_4368 at 4825.583 kb on + strand at 4825.584 kb on - strand, within Pf1N1B4_4368 at 4825.584 kb on - strand, within Pf1N1B4_4368 at 4825.584 kb on - strand, within Pf1N1B4_4368 at 4825.584 kb on - strand, within Pf1N1B4_4368 at 4825.648 kb on + strand, within Pf1N1B4_4368 at 4825.649 kb on - strand, within Pf1N1B4_4368 at 4825.649 kb on - strand, within Pf1N1B4_4368 at 4826.022 kb on + strand at 4826.122 kb on + strand at 4826.283 kb on - strand, within Pf1N1B4_4369 at 4826.283 kb on - strand, within Pf1N1B4_4369 at 4826.285 kb on - strand, within Pf1N1B4_4369 at 4826.285 kb on - strand, within Pf1N1B4_4369 at 4826.348 kb on + strand, within Pf1N1B4_4369 at 4826.348 kb on + strand, within Pf1N1B4_4369 at 4826.348 kb on + strand, within Pf1N1B4_4369 at 4826.382 kb on + strand, within Pf1N1B4_4369 at 4826.382 kb on + strand, within Pf1N1B4_4369 at 4826.383 kb on - strand, within Pf1N1B4_4369 at 4826.588 kb on + strand, within Pf1N1B4_4369 at 4826.588 kb on + strand, within Pf1N1B4_4369 at 4826.589 kb on - strand, within Pf1N1B4_4369 at 4826.589 kb on - strand, within Pf1N1B4_4369 at 4826.648 kb on + strand, within Pf1N1B4_4369 at 4826.649 kb on - strand, within Pf1N1B4_4369 at 4826.796 kb on - strand, within Pf1N1B4_4369
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,824,608 + +0.2 4,824,608 + +0.5 4,824,608 + -1.1 4,824,609 - -0.0 4,824,679 + -0.3 4,824,680 - -0.5 4,824,715 + +0.5 4,824,715 + +0.5 4,824,716 - +0.1 4,824,716 - +1.5 4,824,737 - -0.8 4,824,737 - -2.8 4,824,744 - +1.5 4,824,792 + +0.5 4,824,793 - +1.5 4,824,871 + -2.5 4,824,871 + -0.5 4,824,871 + +0.2 4,824,977 + +0.7 4,824,978 - -0.7 4,824,978 - +0.1 4,824,978 - +0.2 4,824,978 - -2.1 4,825,063 + -0.5 4,825,197 + Pf1N1B4_4367 0.17 +0.3 4,825,197 + Pf1N1B4_4367 0.17 -2.5 4,825,581 + Pf1N1B4_4368 0.18 -2.1 4,825,581 + Pf1N1B4_4368 0.18 -1.5 4,825,583 + Pf1N1B4_4368 0.19 -2.1 4,825,583 + +0.1 4,825,583 + Pf1N1B4_4368 0.19 -0.1 4,825,583 + -1.5 4,825,584 - Pf1N1B4_4368 0.19 -0.2 4,825,584 - Pf1N1B4_4368 0.19 -1.5 4,825,584 - Pf1N1B4_4368 0.19 -2.3 4,825,584 - Pf1N1B4_4368 0.19 -2.8 4,825,648 + Pf1N1B4_4368 0.34 +1.1 4,825,649 - Pf1N1B4_4368 0.35 +0.5 4,825,649 - Pf1N1B4_4368 0.35 -1.5 4,826,022 + -2.1 4,826,122 + -0.1 4,826,283 - Pf1N1B4_4369 0.15 +0.1 4,826,283 - Pf1N1B4_4369 0.15 -0.5 4,826,285 - Pf1N1B4_4369 0.15 +1.5 4,826,285 - Pf1N1B4_4369 0.15 -0.8 4,826,348 + Pf1N1B4_4369 0.20 -1.3 4,826,348 + Pf1N1B4_4369 0.20 +1.4 4,826,348 + Pf1N1B4_4369 0.20 -0.5 4,826,382 + Pf1N1B4_4369 0.23 +1.8 4,826,382 + Pf1N1B4_4369 0.23 -0.1 4,826,383 - Pf1N1B4_4369 0.23 -0.1 4,826,588 + Pf1N1B4_4369 0.38 -0.5 4,826,588 + Pf1N1B4_4369 0.38 +1.8 4,826,589 - Pf1N1B4_4369 0.38 -0.5 4,826,589 - Pf1N1B4_4369 0.38 +0.5 4,826,648 + Pf1N1B4_4369 0.43 +0.2 4,826,649 - Pf1N1B4_4369 0.43 +0.5 4,826,796 - Pf1N1B4_4369 0.54 +0.2
Or see this region's nucleotide sequence