Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4348 and Pf1N1B4_4349 are separated by 406 nucleotides Pf1N1B4_4349 and Pf1N1B4_4350 are separated by 89 nucleotides Pf1N1B4_4350 and Pf1N1B4_4351 overlap by 4 nucleotides
Pf1N1B4_4348 - 2'-5' RNA ligase, at 4,804,061 to 4,804,600
_4348
Pf1N1B4_4349 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 4,805,007 to 4,806,104
_4349
Pf1N1B4_4350 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 4,806,194 to 4,807,078
_4350
Pf1N1B4_4351 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 4,807,075 to 4,807,989
_4351
Position (kb)
4805
4806
4807 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4804.389 kb on - strand, within Pf1N1B4_4348 at 4804.534 kb on + strand, within Pf1N1B4_4348 at 4804.535 kb on - strand, within Pf1N1B4_4348 at 4804.535 kb on - strand, within Pf1N1B4_4348 at 4804.535 kb on - strand at 4804.598 kb on + strand at 4804.598 kb on + strand at 4804.599 kb on - strand at 4804.871 kb on + strand at 4804.872 kb on - strand at 4804.872 kb on - strand at 4804.872 kb on - strand at 4804.872 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4805.012 kb on + strand at 4805.083 kb on + strand at 4805.083 kb on + strand at 4805.083 kb on + strand at 4805.084 kb on - strand at 4805.084 kb on - strand at 4805.084 kb on - strand at 4805.084 kb on - strand at 4805.084 kb on - strand at 4805.242 kb on + strand at 4805.390 kb on - strand, within Pf1N1B4_4349 at 4805.434 kb on + strand, within Pf1N1B4_4349 at 4805.435 kb on - strand, within Pf1N1B4_4349 at 4805.435 kb on - strand, within Pf1N1B4_4349 at 4805.453 kb on - strand, within Pf1N1B4_4349 at 4805.720 kb on - strand, within Pf1N1B4_4349 at 4805.720 kb on - strand, within Pf1N1B4_4349 at 4805.824 kb on + strand at 4805.825 kb on - strand, within Pf1N1B4_4349 at 4805.924 kb on + strand, within Pf1N1B4_4349 at 4805.942 kb on - strand at 4805.998 kb on + strand at 4806.066 kb on + strand at 4806.067 kb on - strand at 4806.127 kb on + strand at 4806.128 kb on - strand at 4806.139 kb on + strand at 4806.139 kb on + strand at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.797 kb on - strand, within Pf1N1B4_4350 at 4806.874 kb on - strand, within Pf1N1B4_4350 at 4806.940 kb on - strand, within Pf1N1B4_4350 at 4806.940 kb on - strand, within Pf1N1B4_4350 at 4807.024 kb on - strand at 4807.024 kb on - strand at 4807.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,804,389 - Pf1N1B4_4348 0.61 +0.9 4,804,534 + Pf1N1B4_4348 0.88 -1.5 4,804,535 - Pf1N1B4_4348 0.88 -1.1 4,804,535 - Pf1N1B4_4348 0.88 +0.1 4,804,535 - +0.5 4,804,598 + -0.0 4,804,598 + +0.5 4,804,599 - +0.4 4,804,871 + -1.5 4,804,872 - +1.1 4,804,872 - -2.1 4,804,872 - -1.5 4,804,872 - -2.5 4,804,947 - -1.5 4,804,947 - -2.3 4,804,947 - -0.6 4,804,947 - -1.0 4,804,947 - -0.5 4,804,947 - +0.0 4,805,012 + -0.1 4,805,083 + -0.9 4,805,083 + +0.3 4,805,083 + -0.5 4,805,084 - +0.1 4,805,084 - -0.9 4,805,084 - +0.7 4,805,084 - -0.5 4,805,084 - +1.8 4,805,242 + -0.5 4,805,390 - Pf1N1B4_4349 0.35 +0.5 4,805,434 + Pf1N1B4_4349 0.39 +1.3 4,805,435 - Pf1N1B4_4349 0.39 -0.1 4,805,435 - Pf1N1B4_4349 0.39 -0.7 4,805,453 - Pf1N1B4_4349 0.41 +0.1 4,805,720 - Pf1N1B4_4349 0.65 +1.1 4,805,720 - Pf1N1B4_4349 0.65 -0.5 4,805,824 + -0.5 4,805,825 - Pf1N1B4_4349 0.74 +0.5 4,805,924 + Pf1N1B4_4349 0.84 +1.5 4,805,942 - +0.1 4,805,998 + +1.5 4,806,066 + +0.5 4,806,067 - +0.5 4,806,127 + -0.5 4,806,128 - -1.5 4,806,139 + -0.0 4,806,139 + +0.5 4,806,633 + Pf1N1B4_4350 0.50 +0.8 4,806,633 + Pf1N1B4_4350 0.50 -0.2 4,806,633 + Pf1N1B4_4350 0.50 +0.5 4,806,633 + Pf1N1B4_4350 0.50 -0.7 4,806,633 + Pf1N1B4_4350 0.50 -0.8 4,806,634 - Pf1N1B4_4350 0.50 +0.3 4,806,634 - Pf1N1B4_4350 0.50 -1.1 4,806,634 - Pf1N1B4_4350 0.50 +1.3 4,806,634 - Pf1N1B4_4350 0.50 +2.1 4,806,634 - Pf1N1B4_4350 0.50 -0.5 4,806,797 - Pf1N1B4_4350 0.68 -1.1 4,806,874 - Pf1N1B4_4350 0.77 +0.3 4,806,940 - Pf1N1B4_4350 0.84 +0.1 4,806,940 - Pf1N1B4_4350 0.84 -0.5 4,807,024 - +0.3 4,807,024 - -0.5 4,807,028 - -2.4
Or see this region's nucleotide sequence