Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4339 and Pf1N1B4_4340 are separated by 37 nucleotides Pf1N1B4_4340 and Pf1N1B4_4341 are separated by 103 nucleotides Pf1N1B4_4341 and Pf1N1B4_4342 are separated by 364 nucleotides Pf1N1B4_4342 and Pf1N1B4_4343 are separated by 27 nucleotides Pf1N1B4_4343 and Pf1N1B4_4344 overlap by 4 nucleotides
Pf1N1B4_4339 - Malic enzyme, at 4,798,331 to 4,799,551
_4339
Pf1N1B4_4340 - FOG: WD40-like repeat, at 4,799,589 to 4,800,218
_4340
Pf1N1B4_4341 - hypothetical protein, at 4,800,322 to 4,801,020
_4341
Pf1N1B4_4342 - hypothetical protein, at 4,801,385 to 4,801,528
_4342
Pf1N1B4_4343 - hypothetical protein, at 4,801,556 to 4,801,825
_4343
Pf1N1B4_4344 - hypothetical protein, at 4,801,822 to 4,801,977
_4344
Position (kb)
4800
4801
4802 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4799.517 kb on + strand at 4799.518 kb on - strand at 4799.524 kb on - strand at 4799.550 kb on - strand at 4799.622 kb on + strand at 4799.845 kb on + strand at 4799.845 kb on + strand, within Pf1N1B4_4340 at 4800.031 kb on + strand, within Pf1N1B4_4340 at 4800.032 kb on - strand, within Pf1N1B4_4340 at 4800.453 kb on + strand, within Pf1N1B4_4341 at 4800.454 kb on - strand at 4800.578 kb on + strand, within Pf1N1B4_4341 at 4800.610 kb on + strand, within Pf1N1B4_4341 at 4800.611 kb on - strand, within Pf1N1B4_4341 at 4800.616 kb on + strand, within Pf1N1B4_4341 at 4800.616 kb on + strand, within Pf1N1B4_4341 at 4800.616 kb on + strand, within Pf1N1B4_4341 at 4800.616 kb on + strand, within Pf1N1B4_4341 at 4800.631 kb on + strand at 4800.632 kb on - strand, within Pf1N1B4_4341 at 4800.701 kb on - strand, within Pf1N1B4_4341 at 4800.724 kb on + strand at 4800.724 kb on + strand, within Pf1N1B4_4341 at 4800.725 kb on - strand, within Pf1N1B4_4341 at 4801.228 kb on + strand at 4801.238 kb on + strand at 4801.238 kb on + strand at 4801.274 kb on + strand at 4801.343 kb on + strand at 4801.347 kb on + strand at 4801.348 kb on - strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.360 kb on - strand at 4801.360 kb on - strand at 4801.380 kb on + strand at 4801.396 kb on - strand at 4801.614 kb on - strand, within Pf1N1B4_4343 at 4801.770 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.774 kb on - strand, within Pf1N1B4_4343 at 4801.787 kb on + strand, within Pf1N1B4_4343 at 4801.955 kb on + strand, within Pf1N1B4_4344 at 4802.015 kb on + strand at 4802.015 kb on + strand at 4802.016 kb on - strand at 4802.019 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,799,517 + -1.7 4,799,518 - -1.2 4,799,524 - -0.6 4,799,550 - -1.2 4,799,622 + -0.5 4,799,845 + -2.1 4,799,845 + Pf1N1B4_4340 0.41 +0.5 4,800,031 + Pf1N1B4_4340 0.70 +0.1 4,800,032 - Pf1N1B4_4340 0.70 +0.5 4,800,453 + Pf1N1B4_4341 0.19 +2.1 4,800,454 - -0.5 4,800,578 + Pf1N1B4_4341 0.37 -0.2 4,800,610 + Pf1N1B4_4341 0.41 +1.8 4,800,611 - Pf1N1B4_4341 0.41 -0.1 4,800,616 + Pf1N1B4_4341 0.42 -2.1 4,800,616 + Pf1N1B4_4341 0.42 -0.5 4,800,616 + Pf1N1B4_4341 0.42 +1.5 4,800,616 + Pf1N1B4_4341 0.42 +0.3 4,800,631 + -0.5 4,800,632 - Pf1N1B4_4341 0.44 +0.1 4,800,701 - Pf1N1B4_4341 0.54 +1.1 4,800,724 + -2.1 4,800,724 + Pf1N1B4_4341 0.58 -0.1 4,800,725 - Pf1N1B4_4341 0.58 -0.2 4,801,228 + -0.8 4,801,238 + +0.1 4,801,238 + +1.6 4,801,274 + -0.3 4,801,343 + +0.3 4,801,347 + -1.1 4,801,348 - +0.3 4,801,359 + +0.8 4,801,359 + +1.1 4,801,359 + -0.5 4,801,359 + -0.5 4,801,359 + +1.5 4,801,360 - -0.1 4,801,360 - -0.2 4,801,380 + +0.1 4,801,396 - -0.5 4,801,614 - Pf1N1B4_4343 0.21 +0.9 4,801,770 + Pf1N1B4_4343 0.79 +0.5 4,801,773 + Pf1N1B4_4343 0.80 -1.5 4,801,773 + Pf1N1B4_4343 0.80 -0.5 4,801,773 + -1.5 4,801,773 + Pf1N1B4_4343 0.80 +0.5 4,801,773 + Pf1N1B4_4343 0.80 -0.8 4,801,773 + Pf1N1B4_4343 0.80 -1.8 4,801,774 - Pf1N1B4_4343 0.81 +0.5 4,801,787 + Pf1N1B4_4343 0.86 +0.5 4,801,955 + Pf1N1B4_4344 0.85 -0.7 4,802,015 + +0.1 4,802,015 + +0.5 4,802,016 - +0.4 4,802,019 + +0.5
Or see this region's nucleotide sequence