Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4303

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4301 and Pf1N1B4_4302 are separated by 48 nucleotidesPf1N1B4_4302 and Pf1N1B4_4303 are separated by 170 nucleotidesPf1N1B4_4303 and Pf1N1B4_4304 are separated by 188 nucleotidesPf1N1B4_4304 and Pf1N1B4_4305 are separated by 37 nucleotides Pf1N1B4_4301 - hypothetical protein, at 4,765,198 to 4,765,461 _4301 Pf1N1B4_4302 - hypothetical protein, at 4,765,510 to 4,765,731 _4302 Pf1N1B4_4303 - DipZ protein, at 4,765,902 to 4,767,113 _4303 Pf1N1B4_4304 - hypothetical protein, at 4,767,302 to 4,767,574 _4304 Pf1N1B4_4305 - PUTATIVE METHYLTRANSFERASE, at 4,767,612 to 4,768,424 _4305 Position (kb) 4765 4766 4767 4768Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4765.034 kb on + strandat 4765.174 kb on + strandat 4765.175 kb on - strandat 4765.175 kb on - strandat 4765.175 kb on - strandat 4765.294 kb on + strand, within Pf1N1B4_4301at 4765.294 kb on + strand, within Pf1N1B4_4301at 4765.295 kb on - strand, within Pf1N1B4_4301at 4765.295 kb on - strand, within Pf1N1B4_4301at 4765.301 kb on - strand, within Pf1N1B4_4301at 4765.387 kb on + strand, within Pf1N1B4_4301at 4765.388 kb on - strand, within Pf1N1B4_4301at 4765.423 kb on + strand, within Pf1N1B4_4301at 4765.423 kb on + strand, within Pf1N1B4_4301at 4765.424 kb on - strand, within Pf1N1B4_4301at 4765.721 kb on + strandat 4765.721 kb on + strandat 4765.884 kb on - strandat 4765.884 kb on - strandat 4765.884 kb on - strandat 4766.374 kb on - strand, within Pf1N1B4_4303at 4766.616 kb on + strand, within Pf1N1B4_4303at 4766.766 kb on + strand, within Pf1N1B4_4303at 4766.767 kb on - strand, within Pf1N1B4_4303at 4766.767 kb on - strand, within Pf1N1B4_4303at 4766.767 kb on - strand, within Pf1N1B4_4303at 4766.800 kb on - strand, within Pf1N1B4_4303at 4766.869 kb on - strand, within Pf1N1B4_4303at 4766.948 kb on + strand, within Pf1N1B4_4303at 4766.948 kb on + strand, within Pf1N1B4_4303at 4766.948 kb on + strand, within Pf1N1B4_4303at 4766.949 kb on - strand, within Pf1N1B4_4303at 4766.998 kb on - strandat 4767.033 kb on + strandat 4767.033 kb on + strandat 4767.034 kb on - strandat 4767.034 kb on - strandat 4767.160 kb on + strandat 4767.307 kb on + strandat 4767.308 kb on - strandat 4767.311 kb on + strandat 4767.312 kb on - strandat 4767.312 kb on - strandat 4767.434 kb on + strand, within Pf1N1B4_4304at 4767.434 kb on + strand, within Pf1N1B4_4304at 4767.434 kb on + strand, within Pf1N1B4_4304at 4767.435 kb on - strandat 4767.435 kb on - strand, within Pf1N1B4_4304at 4767.435 kb on - strand, within Pf1N1B4_4304at 4767.456 kb on - strandat 4767.462 kb on + strand, within Pf1N1B4_4304at 4767.464 kb on + strandat 4767.464 kb on + strand, within Pf1N1B4_4304at 4767.465 kb on - strandat 4767.465 kb on - strandat 4767.618 kb on - strandat 4767.692 kb on + strandat 4767.700 kb on + strand, within Pf1N1B4_4305at 4767.700 kb on + strand, within Pf1N1B4_4305at 4767.701 kb on - strand, within Pf1N1B4_4305at 4767.701 kb on - strand, within Pf1N1B4_4305at 4767.834 kb on + strand, within Pf1N1B4_4305at 4767.834 kb on + strand, within Pf1N1B4_4305

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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4,765,034 + -0.9
4,765,174 + -1.9
4,765,175 - -0.5
4,765,175 - +1.5
4,765,175 - +0.1
4,765,294 + Pf1N1B4_4301 0.36 -0.3
4,765,294 + Pf1N1B4_4301 0.36 -0.5
4,765,295 - Pf1N1B4_4301 0.37 +0.1
4,765,295 - Pf1N1B4_4301 0.37 -1.5
4,765,301 - Pf1N1B4_4301 0.39 -0.7
4,765,387 + Pf1N1B4_4301 0.72 +0.5
4,765,388 - Pf1N1B4_4301 0.72 -0.2
4,765,423 + Pf1N1B4_4301 0.85 +0.3
4,765,423 + Pf1N1B4_4301 0.85 -1.3
4,765,424 - Pf1N1B4_4301 0.86 -2.5
4,765,721 + -0.5
4,765,721 + -0.3
4,765,884 - -1.1
4,765,884 - -0.1
4,765,884 - +1.3
4,766,374 - Pf1N1B4_4303 0.39 +0.5
4,766,616 + Pf1N1B4_4303 0.59 +1.1
4,766,766 + Pf1N1B4_4303 0.71 -0.5
4,766,767 - Pf1N1B4_4303 0.71 +0.7
4,766,767 - Pf1N1B4_4303 0.71 +0.2
4,766,767 - Pf1N1B4_4303 0.71 -0.5
4,766,800 - Pf1N1B4_4303 0.74 -0.5
4,766,869 - Pf1N1B4_4303 0.80 +1.8
4,766,948 + Pf1N1B4_4303 0.86 -1.7
4,766,948 + Pf1N1B4_4303 0.86 +1.1
4,766,948 + Pf1N1B4_4303 0.86 +0.1
4,766,949 - Pf1N1B4_4303 0.86 +0.5
4,766,998 - -0.2
4,767,033 + -0.5
4,767,033 + -3.3
4,767,034 - -1.9
4,767,034 - -0.9
4,767,160 + -0.7
4,767,307 + -1.7
4,767,308 - +1.1
4,767,311 + +0.5
4,767,312 - -0.5
4,767,312 - -0.5
4,767,434 + Pf1N1B4_4304 0.48 +0.1
4,767,434 + Pf1N1B4_4304 0.48 +1.8
4,767,434 + Pf1N1B4_4304 0.48 -0.5
4,767,435 - -0.5
4,767,435 - Pf1N1B4_4304 0.49 -2.1
4,767,435 - Pf1N1B4_4304 0.49 -1.1
4,767,456 - -0.5
4,767,462 + Pf1N1B4_4304 0.59 +0.4
4,767,464 + +0.1
4,767,464 + Pf1N1B4_4304 0.59 -1.0
4,767,465 - -0.5
4,767,465 - -1.5
4,767,618 - -0.7
4,767,692 + +1.8
4,767,700 + Pf1N1B4_4305 0.11 +0.3
4,767,700 + Pf1N1B4_4305 0.11 +0.1
4,767,701 - Pf1N1B4_4305 0.11 -1.7
4,767,701 - Pf1N1B4_4305 0.11 +0.3
4,767,834 + Pf1N1B4_4305 0.27 -0.2
4,767,834 + Pf1N1B4_4305 0.27 -0.5

Or see this region's nucleotide sequence