Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4301 and Pf1N1B4_4302 are separated by 48 nucleotides Pf1N1B4_4302 and Pf1N1B4_4303 are separated by 170 nucleotides Pf1N1B4_4303 and Pf1N1B4_4304 are separated by 188 nucleotides Pf1N1B4_4304 and Pf1N1B4_4305 are separated by 37 nucleotides
Pf1N1B4_4301 - hypothetical protein, at 4,765,198 to 4,765,461
_4301
Pf1N1B4_4302 - hypothetical protein, at 4,765,510 to 4,765,731
_4302
Pf1N1B4_4303 - DipZ protein, at 4,765,902 to 4,767,113
_4303
Pf1N1B4_4304 - hypothetical protein, at 4,767,302 to 4,767,574
_4304
Pf1N1B4_4305 - PUTATIVE METHYLTRANSFERASE, at 4,767,612 to 4,768,424
_4305
Position (kb)
4765
4766
4767
4768 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4765.034 kb on + strand at 4765.174 kb on + strand at 4765.175 kb on - strand at 4765.175 kb on - strand at 4765.175 kb on - strand at 4765.294 kb on + strand, within Pf1N1B4_4301 at 4765.294 kb on + strand, within Pf1N1B4_4301 at 4765.295 kb on - strand, within Pf1N1B4_4301 at 4765.295 kb on - strand, within Pf1N1B4_4301 at 4765.301 kb on - strand, within Pf1N1B4_4301 at 4765.387 kb on + strand, within Pf1N1B4_4301 at 4765.388 kb on - strand, within Pf1N1B4_4301 at 4765.423 kb on + strand, within Pf1N1B4_4301 at 4765.423 kb on + strand, within Pf1N1B4_4301 at 4765.424 kb on - strand, within Pf1N1B4_4301 at 4765.721 kb on + strand at 4765.721 kb on + strand at 4765.884 kb on - strand at 4765.884 kb on - strand at 4765.884 kb on - strand at 4766.374 kb on - strand, within Pf1N1B4_4303 at 4766.616 kb on + strand, within Pf1N1B4_4303 at 4766.766 kb on + strand, within Pf1N1B4_4303 at 4766.767 kb on - strand, within Pf1N1B4_4303 at 4766.767 kb on - strand, within Pf1N1B4_4303 at 4766.767 kb on - strand, within Pf1N1B4_4303 at 4766.800 kb on - strand, within Pf1N1B4_4303 at 4766.869 kb on - strand, within Pf1N1B4_4303 at 4766.948 kb on + strand, within Pf1N1B4_4303 at 4766.948 kb on + strand, within Pf1N1B4_4303 at 4766.948 kb on + strand, within Pf1N1B4_4303 at 4766.949 kb on - strand, within Pf1N1B4_4303 at 4766.998 kb on - strand at 4767.033 kb on + strand at 4767.033 kb on + strand at 4767.034 kb on - strand at 4767.034 kb on - strand at 4767.160 kb on + strand at 4767.307 kb on + strand at 4767.308 kb on - strand at 4767.311 kb on + strand at 4767.312 kb on - strand at 4767.312 kb on - strand at 4767.434 kb on + strand, within Pf1N1B4_4304 at 4767.434 kb on + strand, within Pf1N1B4_4304 at 4767.434 kb on + strand, within Pf1N1B4_4304 at 4767.435 kb on - strand at 4767.435 kb on - strand, within Pf1N1B4_4304 at 4767.435 kb on - strand, within Pf1N1B4_4304 at 4767.456 kb on - strand at 4767.462 kb on + strand, within Pf1N1B4_4304 at 4767.464 kb on + strand at 4767.464 kb on + strand, within Pf1N1B4_4304 at 4767.465 kb on - strand at 4767.465 kb on - strand at 4767.618 kb on - strand at 4767.692 kb on + strand at 4767.700 kb on + strand, within Pf1N1B4_4305 at 4767.700 kb on + strand, within Pf1N1B4_4305 at 4767.701 kb on - strand, within Pf1N1B4_4305 at 4767.701 kb on - strand, within Pf1N1B4_4305 at 4767.834 kb on + strand, within Pf1N1B4_4305 at 4767.834 kb on + strand, within Pf1N1B4_4305
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,765,034 + -0.9 4,765,174 + -1.9 4,765,175 - -0.5 4,765,175 - +1.5 4,765,175 - +0.1 4,765,294 + Pf1N1B4_4301 0.36 -0.3 4,765,294 + Pf1N1B4_4301 0.36 -0.5 4,765,295 - Pf1N1B4_4301 0.37 +0.1 4,765,295 - Pf1N1B4_4301 0.37 -1.5 4,765,301 - Pf1N1B4_4301 0.39 -0.7 4,765,387 + Pf1N1B4_4301 0.72 +0.5 4,765,388 - Pf1N1B4_4301 0.72 -0.2 4,765,423 + Pf1N1B4_4301 0.85 +0.3 4,765,423 + Pf1N1B4_4301 0.85 -1.3 4,765,424 - Pf1N1B4_4301 0.86 -2.5 4,765,721 + -0.5 4,765,721 + -0.3 4,765,884 - -1.1 4,765,884 - -0.1 4,765,884 - +1.3 4,766,374 - Pf1N1B4_4303 0.39 +0.5 4,766,616 + Pf1N1B4_4303 0.59 +1.1 4,766,766 + Pf1N1B4_4303 0.71 -0.5 4,766,767 - Pf1N1B4_4303 0.71 +0.7 4,766,767 - Pf1N1B4_4303 0.71 +0.2 4,766,767 - Pf1N1B4_4303 0.71 -0.5 4,766,800 - Pf1N1B4_4303 0.74 -0.5 4,766,869 - Pf1N1B4_4303 0.80 +1.8 4,766,948 + Pf1N1B4_4303 0.86 -1.7 4,766,948 + Pf1N1B4_4303 0.86 +1.1 4,766,948 + Pf1N1B4_4303 0.86 +0.1 4,766,949 - Pf1N1B4_4303 0.86 +0.5 4,766,998 - -0.2 4,767,033 + -0.5 4,767,033 + -3.3 4,767,034 - -1.9 4,767,034 - -0.9 4,767,160 + -0.7 4,767,307 + -1.7 4,767,308 - +1.1 4,767,311 + +0.5 4,767,312 - -0.5 4,767,312 - -0.5 4,767,434 + Pf1N1B4_4304 0.48 +0.1 4,767,434 + Pf1N1B4_4304 0.48 +1.8 4,767,434 + Pf1N1B4_4304 0.48 -0.5 4,767,435 - -0.5 4,767,435 - Pf1N1B4_4304 0.49 -2.1 4,767,435 - Pf1N1B4_4304 0.49 -1.1 4,767,456 - -0.5 4,767,462 + Pf1N1B4_4304 0.59 +0.4 4,767,464 + +0.1 4,767,464 + Pf1N1B4_4304 0.59 -1.0 4,767,465 - -0.5 4,767,465 - -1.5 4,767,618 - -0.7 4,767,692 + +1.8 4,767,700 + Pf1N1B4_4305 0.11 +0.3 4,767,700 + Pf1N1B4_4305 0.11 +0.1 4,767,701 - Pf1N1B4_4305 0.11 -1.7 4,767,701 - Pf1N1B4_4305 0.11 +0.3 4,767,834 + Pf1N1B4_4305 0.27 -0.2 4,767,834 + Pf1N1B4_4305 0.27 -0.5
Or see this region's nucleotide sequence