Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4285

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4282 and Pf1N1B4_4283 are separated by 4 nucleotidesPf1N1B4_4283 and Pf1N1B4_4284 are separated by 33 nucleotidesPf1N1B4_4284 and Pf1N1B4_4285 are separated by 78 nucleotidesPf1N1B4_4285 and Pf1N1B4_4286 are separated by 54 nucleotides Pf1N1B4_4282 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,748,892 to 4,749,944 _4282 Pf1N1B4_4283 - hypothetical protein, at 4,749,949 to 4,750,065 _4283 Pf1N1B4_4284 - hypothetical protein, at 4,750,099 to 4,750,233 _4284 Pf1N1B4_4285 - Inositol transport system sugar-binding protein, at 4,750,312 to 4,751,238 _4285 Pf1N1B4_4286 - Inositol transport system ATP-binding protein, at 4,751,293 to 4,752,873 _4286 Position (kb) 4750 4751 4752Strain fitness (log2 ratio) -2 -1 0 1 2at 4749.457 kb on - strand, within Pf1N1B4_4282at 4749.471 kb on + strand, within Pf1N1B4_4282at 4749.509 kb on + strand, within Pf1N1B4_4282at 4749.510 kb on - strandat 4749.510 kb on - strand, within Pf1N1B4_4282at 4749.510 kb on - strand, within Pf1N1B4_4282at 4749.613 kb on - strand, within Pf1N1B4_4282at 4749.781 kb on - strand, within Pf1N1B4_4282at 4749.781 kb on - strand, within Pf1N1B4_4282at 4749.790 kb on + strand, within Pf1N1B4_4282at 4750.121 kb on - strand, within Pf1N1B4_4284at 4750.221 kb on - strandat 4750.279 kb on + strandat 4750.383 kb on + strandat 4750.432 kb on + strand, within Pf1N1B4_4285at 4750.535 kb on + strand, within Pf1N1B4_4285at 4750.645 kb on + strandat 4750.645 kb on + strand, within Pf1N1B4_4285at 4750.645 kb on + strand, within Pf1N1B4_4285at 4750.739 kb on - strand, within Pf1N1B4_4285at 4750.849 kb on + strand, within Pf1N1B4_4285at 4751.236 kb on + strandat 4751.237 kb on - strandat 4751.261 kb on + strandat 4751.261 kb on + strandat 4751.262 kb on - strandat 4751.262 kb on - strandat 4751.262 kb on - strandat 4751.299 kb on + strandat 4751.383 kb on + strandat 4751.536 kb on + strand, within Pf1N1B4_4286at 4751.849 kb on - strand, within Pf1N1B4_4286at 4751.962 kb on + strand, within Pf1N1B4_4286at 4751.962 kb on + strand, within Pf1N1B4_4286at 4751.962 kb on + strand, within Pf1N1B4_4286at 4751.962 kb on + strand, within Pf1N1B4_4286at 4751.962 kb on + strand, within Pf1N1B4_4286at 4751.963 kb on - strand, within Pf1N1B4_4286at 4751.963 kb on - strand, within Pf1N1B4_4286at 4751.963 kb on - strand, within Pf1N1B4_4286at 4752.031 kb on + strand, within Pf1N1B4_4286at 4752.031 kb on + strand, within Pf1N1B4_4286at 4752.031 kb on + strand, within Pf1N1B4_4286at 4752.032 kb on - strand, within Pf1N1B4_4286at 4752.032 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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4,749,457 - Pf1N1B4_4282 0.54 +0.1
4,749,471 + Pf1N1B4_4282 0.55 +0.1
4,749,509 + Pf1N1B4_4282 0.59 -0.3
4,749,510 - +1.1
4,749,510 - Pf1N1B4_4282 0.59 -2.1
4,749,510 - Pf1N1B4_4282 0.59 -1.7
4,749,613 - Pf1N1B4_4282 0.68 -2.5
4,749,781 - Pf1N1B4_4282 0.84 -0.5
4,749,781 - Pf1N1B4_4282 0.84 +0.5
4,749,790 + Pf1N1B4_4282 0.85 +1.1
4,750,121 - Pf1N1B4_4284 0.16 +0.5
4,750,221 - +0.5
4,750,279 + +0.5
4,750,383 + +0.5
4,750,432 + Pf1N1B4_4285 0.13 -0.3
4,750,535 + Pf1N1B4_4285 0.24 -2.5
4,750,645 + -1.5
4,750,645 + Pf1N1B4_4285 0.36 -0.5
4,750,645 + Pf1N1B4_4285 0.36 -1.5
4,750,739 - Pf1N1B4_4285 0.46 -2.5
4,750,849 + Pf1N1B4_4285 0.58 -1.9
4,751,236 + +0.1
4,751,237 - +0.5
4,751,261 + -0.7
4,751,261 + -1.5
4,751,262 - -0.8
4,751,262 - +1.7
4,751,262 - -0.8
4,751,299 + -0.2
4,751,383 + +0.2
4,751,536 + Pf1N1B4_4286 0.15 +0.2
4,751,849 - Pf1N1B4_4286 0.35 +0.5
4,751,962 + Pf1N1B4_4286 0.42 -0.8
4,751,962 + Pf1N1B4_4286 0.42 -0.5
4,751,962 + Pf1N1B4_4286 0.42 +1.0
4,751,962 + Pf1N1B4_4286 0.42 -0.9
4,751,962 + Pf1N1B4_4286 0.42 +2.5
4,751,963 - Pf1N1B4_4286 0.42 -0.1
4,751,963 - Pf1N1B4_4286 0.42 -0.5
4,751,963 - Pf1N1B4_4286 0.42 -0.8
4,752,031 + Pf1N1B4_4286 0.47 +1.5
4,752,031 + Pf1N1B4_4286 0.47 -0.2
4,752,031 + Pf1N1B4_4286 0.47 +0.8
4,752,032 - Pf1N1B4_4286 0.47 -1.1
4,752,032 - -0.5

Or see this region's nucleotide sequence