Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4282 and Pf1N1B4_4283 are separated by 4 nucleotides Pf1N1B4_4283 and Pf1N1B4_4284 are separated by 33 nucleotides Pf1N1B4_4284 and Pf1N1B4_4285 are separated by 78 nucleotides Pf1N1B4_4285 and Pf1N1B4_4286 are separated by 54 nucleotides
Pf1N1B4_4282 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,748,892 to 4,749,944
_4282
Pf1N1B4_4283 - hypothetical protein, at 4,749,949 to 4,750,065
_4283
Pf1N1B4_4284 - hypothetical protein, at 4,750,099 to 4,750,233
_4284
Pf1N1B4_4285 - Inositol transport system sugar-binding protein, at 4,750,312 to 4,751,238
_4285
Pf1N1B4_4286 - Inositol transport system ATP-binding protein, at 4,751,293 to 4,752,873
_4286
Position (kb)
4750
4751
4752 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4749.457 kb on - strand, within Pf1N1B4_4282 at 4749.471 kb on + strand, within Pf1N1B4_4282 at 4749.509 kb on + strand, within Pf1N1B4_4282 at 4749.510 kb on - strand at 4749.510 kb on - strand, within Pf1N1B4_4282 at 4749.510 kb on - strand, within Pf1N1B4_4282 at 4749.613 kb on - strand, within Pf1N1B4_4282 at 4749.781 kb on - strand, within Pf1N1B4_4282 at 4749.781 kb on - strand, within Pf1N1B4_4282 at 4749.790 kb on + strand, within Pf1N1B4_4282 at 4750.121 kb on - strand, within Pf1N1B4_4284 at 4750.221 kb on - strand at 4750.279 kb on + strand at 4750.383 kb on + strand at 4750.432 kb on + strand, within Pf1N1B4_4285 at 4750.535 kb on + strand, within Pf1N1B4_4285 at 4750.645 kb on + strand at 4750.645 kb on + strand, within Pf1N1B4_4285 at 4750.645 kb on + strand, within Pf1N1B4_4285 at 4750.739 kb on - strand, within Pf1N1B4_4285 at 4750.849 kb on + strand, within Pf1N1B4_4285 at 4751.236 kb on + strand at 4751.237 kb on - strand at 4751.261 kb on + strand at 4751.261 kb on + strand at 4751.262 kb on - strand at 4751.262 kb on - strand at 4751.262 kb on - strand at 4751.299 kb on + strand at 4751.383 kb on + strand at 4751.536 kb on + strand, within Pf1N1B4_4286 at 4751.849 kb on - strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.032 kb on - strand, within Pf1N1B4_4286 at 4752.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,749,457 - Pf1N1B4_4282 0.54 +0.1 4,749,471 + Pf1N1B4_4282 0.55 +0.1 4,749,509 + Pf1N1B4_4282 0.59 -0.3 4,749,510 - +1.1 4,749,510 - Pf1N1B4_4282 0.59 -2.1 4,749,510 - Pf1N1B4_4282 0.59 -1.7 4,749,613 - Pf1N1B4_4282 0.68 -2.5 4,749,781 - Pf1N1B4_4282 0.84 -0.5 4,749,781 - Pf1N1B4_4282 0.84 +0.5 4,749,790 + Pf1N1B4_4282 0.85 +1.1 4,750,121 - Pf1N1B4_4284 0.16 +0.5 4,750,221 - +0.5 4,750,279 + +0.5 4,750,383 + +0.5 4,750,432 + Pf1N1B4_4285 0.13 -0.3 4,750,535 + Pf1N1B4_4285 0.24 -2.5 4,750,645 + -1.5 4,750,645 + Pf1N1B4_4285 0.36 -0.5 4,750,645 + Pf1N1B4_4285 0.36 -1.5 4,750,739 - Pf1N1B4_4285 0.46 -2.5 4,750,849 + Pf1N1B4_4285 0.58 -1.9 4,751,236 + +0.1 4,751,237 - +0.5 4,751,261 + -0.7 4,751,261 + -1.5 4,751,262 - -0.8 4,751,262 - +1.7 4,751,262 - -0.8 4,751,299 + -0.2 4,751,383 + +0.2 4,751,536 + Pf1N1B4_4286 0.15 +0.2 4,751,849 - Pf1N1B4_4286 0.35 +0.5 4,751,962 + Pf1N1B4_4286 0.42 -0.8 4,751,962 + Pf1N1B4_4286 0.42 -0.5 4,751,962 + Pf1N1B4_4286 0.42 +1.0 4,751,962 + Pf1N1B4_4286 0.42 -0.9 4,751,962 + Pf1N1B4_4286 0.42 +2.5 4,751,963 - Pf1N1B4_4286 0.42 -0.1 4,751,963 - Pf1N1B4_4286 0.42 -0.5 4,751,963 - Pf1N1B4_4286 0.42 -0.8 4,752,031 + Pf1N1B4_4286 0.47 +1.5 4,752,031 + Pf1N1B4_4286 0.47 -0.2 4,752,031 + Pf1N1B4_4286 0.47 +0.8 4,752,032 - Pf1N1B4_4286 0.47 -1.1 4,752,032 - -0.5
Or see this region's nucleotide sequence