Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4139 and Pf1N1B4_4140 are separated by 941 nucleotides Pf1N1B4_4140 and Pf1N1B4_4141 are separated by 103 nucleotides
Pf1N1B4_4139 - Transcriptional regulator, MerR family, at 4,581,739 to 4,582,152
_4139
Pf1N1B4_4140 - hypothetical protein, at 4,583,094 to 4,583,225
_4140
Pf1N1B4_4141 - putative serine protease, at 4,583,329 to 4,585,803
_4141
Position (kb)
4583
4584 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4582.095 kb on - strand, within Pf1N1B4_4139 at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.144 kb on + strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.145 kb on - strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.148 kb on + strand at 4582.149 kb on - strand at 4582.149 kb on - strand at 4582.149 kb on - strand at 4582.149 kb on - strand at 4582.149 kb on - strand at 4582.149 kb on - strand at 4582.149 kb on - strand at 4582.290 kb on + strand at 4582.290 kb on + strand at 4582.290 kb on + strand at 4582.290 kb on + strand at 4582.290 kb on + strand at 4582.290 kb on + strand at 4582.290 kb on + strand at 4582.291 kb on - strand at 4582.291 kb on - strand at 4582.291 kb on - strand at 4582.291 kb on - strand at 4582.479 kb on - strand at 4582.479 kb on - strand at 4582.517 kb on - strand at 4582.517 kb on - strand at 4582.517 kb on - strand at 4582.698 kb on + strand at 4582.699 kb on - strand at 4582.699 kb on - strand at 4582.699 kb on - strand at 4582.699 kb on - strand at 4582.705 kb on - strand at 4582.705 kb on - strand at 4582.707 kb on - strand at 4582.810 kb on - strand at 4582.834 kb on - strand at 4582.863 kb on - strand at 4582.940 kb on - strand at 4582.941 kb on + strand at 4582.942 kb on - strand at 4583.001 kb on + strand at 4583.002 kb on - strand at 4583.031 kb on - strand at 4583.031 kb on - strand at 4583.031 kb on - strand at 4583.031 kb on - strand at 4583.036 kb on - strand at 4583.036 kb on - strand at 4583.064 kb on - strand at 4583.110 kb on - strand, within Pf1N1B4_4140 at 4583.110 kb on - strand, within Pf1N1B4_4140 at 4583.110 kb on - strand, within Pf1N1B4_4140 at 4583.125 kb on + strand, within Pf1N1B4_4140 at 4583.126 kb on - strand, within Pf1N1B4_4140 at 4583.196 kb on + strand, within Pf1N1B4_4140 at 4583.253 kb on + strand at 4583.287 kb on + strand at 4583.330 kb on + strand at 4583.330 kb on + strand at 4583.331 kb on - strand at 4583.331 kb on - strand at 4583.331 kb on - strand at 4583.366 kb on + strand at 4583.366 kb on + strand at 4583.367 kb on - strand at 4583.367 kb on - strand at 4583.403 kb on + strand at 4583.404 kb on - strand at 4583.404 kb on - strand at 4583.404 kb on - strand at 4583.405 kb on - strand at 4583.479 kb on + strand at 4583.670 kb on + strand, within Pf1N1B4_4141 at 4583.670 kb on + strand, within Pf1N1B4_4141 at 4583.671 kb on - strand at 4583.671 kb on - strand, within Pf1N1B4_4141 at 4583.683 kb on + strand, within Pf1N1B4_4141 at 4583.705 kb on + strand, within Pf1N1B4_4141 at 4583.705 kb on + strand, within Pf1N1B4_4141 at 4583.706 kb on - strand, within Pf1N1B4_4141 at 4583.706 kb on - strand, within Pf1N1B4_4141 at 4583.706 kb on - strand at 4583.706 kb on - strand, within Pf1N1B4_4141 at 4583.706 kb on - strand, within Pf1N1B4_4141 at 4583.750 kb on + strand, within Pf1N1B4_4141 at 4583.750 kb on + strand, within Pf1N1B4_4141 at 4583.751 kb on - strand, within Pf1N1B4_4141 at 4583.781 kb on + strand, within Pf1N1B4_4141 at 4583.781 kb on + strand, within Pf1N1B4_4141 at 4583.845 kb on - strand, within Pf1N1B4_4141 at 4583.868 kb on + strand, within Pf1N1B4_4141 at 4583.868 kb on + strand, within Pf1N1B4_4141 at 4583.868 kb on + strand, within Pf1N1B4_4141 at 4583.868 kb on + strand, within Pf1N1B4_4141 at 4583.868 kb on + strand, within Pf1N1B4_4141 at 4583.869 kb on - strand at 4583.869 kb on - strand, within Pf1N1B4_4141 at 4583.869 kb on - strand, within Pf1N1B4_4141 at 4583.869 kb on - strand, within Pf1N1B4_4141 at 4583.870 kb on + strand, within Pf1N1B4_4141 at 4583.877 kb on - strand, within Pf1N1B4_4141 at 4584.008 kb on + strand, within Pf1N1B4_4141 at 4584.009 kb on - strand, within Pf1N1B4_4141 at 4584.009 kb on - strand, within Pf1N1B4_4141 at 4584.009 kb on - strand, within Pf1N1B4_4141 at 4584.021 kb on + strand, within Pf1N1B4_4141
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,582,095 - Pf1N1B4_4139 0.86 +0.6 4,582,144 + -2.4 4,582,144 + +0.1 4,582,144 + -1.4 4,582,144 + -0.9 4,582,144 + -1.0 4,582,144 + -2.0 4,582,144 + -2.8 4,582,144 + -0.4 4,582,144 + -0.6 4,582,144 + +0.1 4,582,144 + -1.4 4,582,144 + -2.0 4,582,145 - +0.1 4,582,145 - -1.2 4,582,145 - -0.4 4,582,145 - -3.0 4,582,145 - -1.4 4,582,145 - -1.4 4,582,145 - +0.2 4,582,145 - -0.4 4,582,145 - +1.9 4,582,145 - -1.3 4,582,145 - -0.0 4,582,145 - -0.4 4,582,145 - +0.4 4,582,145 - -2.0 4,582,148 + +0.3 4,582,148 + -0.9 4,582,148 + -2.4 4,582,148 + -1.2 4,582,148 + -1.2 4,582,148 + -2.4 4,582,148 + -1.4 4,582,148 + -0.9 4,582,148 + -0.0 4,582,148 + +0.6 4,582,149 - +0.6 4,582,149 - +0.6 4,582,149 - -0.3 4,582,149 - +0.3 4,582,149 - -0.4 4,582,149 - -1.4 4,582,149 - -2.0 4,582,290 + -2.0 4,582,290 + +0.1 4,582,290 + -1.4 4,582,290 + +0.1 4,582,290 + +0.6 4,582,290 + -0.4 4,582,290 + -0.0 4,582,291 - -0.4 4,582,291 - +2.4 4,582,291 - -0.9 4,582,291 - -1.4 4,582,479 - +0.1 4,582,479 - -1.0 4,582,517 - -1.7 4,582,517 - -0.4 4,582,517 - -0.0 4,582,698 + -0.4 4,582,699 - -2.0 4,582,699 - -1.7 4,582,699 - +0.7 4,582,699 - -1.0 4,582,705 - +1.6 4,582,705 - -1.0 4,582,707 - +0.6 4,582,810 - -0.4 4,582,834 - -0.9 4,582,863 - +0.4 4,582,940 - -0.1 4,582,941 + +0.6 4,582,942 - -1.0 4,583,001 + -0.8 4,583,002 - -0.9 4,583,031 - -2.4 4,583,031 - +0.1 4,583,031 - -0.0 4,583,031 - -0.9 4,583,036 - -0.9 4,583,036 - +0.4 4,583,064 - -1.4 4,583,110 - Pf1N1B4_4140 0.12 -0.9 4,583,110 - Pf1N1B4_4140 0.12 -1.0 4,583,110 - Pf1N1B4_4140 0.12 -1.0 4,583,125 + Pf1N1B4_4140 0.23 -1.0 4,583,126 - Pf1N1B4_4140 0.24 -0.4 4,583,196 + Pf1N1B4_4140 0.77 -2.2 4,583,253 + -2.0 4,583,287 + -0.4 4,583,330 + +0.1 4,583,330 + -1.0 4,583,331 - -1.2 4,583,331 - +0.0 4,583,331 - -0.7 4,583,366 + +0.6 4,583,366 + -0.0 4,583,367 - +0.6 4,583,367 - -0.8 4,583,403 + -0.7 4,583,404 - -0.8 4,583,404 - +0.1 4,583,404 - -0.4 4,583,405 - +1.1 4,583,479 + -0.8 4,583,670 + Pf1N1B4_4141 0.14 +0.1 4,583,670 + Pf1N1B4_4141 0.14 -1.0 4,583,671 - -0.4 4,583,671 - Pf1N1B4_4141 0.14 -0.0 4,583,683 + Pf1N1B4_4141 0.14 +0.6 4,583,705 + Pf1N1B4_4141 0.15 -1.4 4,583,705 + Pf1N1B4_4141 0.15 -0.8 4,583,706 - Pf1N1B4_4141 0.15 -0.1 4,583,706 - Pf1N1B4_4141 0.15 -0.7 4,583,706 - -0.4 4,583,706 - Pf1N1B4_4141 0.15 +0.3 4,583,706 - Pf1N1B4_4141 0.15 -1.0 4,583,750 + Pf1N1B4_4141 0.17 -0.4 4,583,750 + Pf1N1B4_4141 0.17 +0.1 4,583,751 - Pf1N1B4_4141 0.17 -1.0 4,583,781 + Pf1N1B4_4141 0.18 -0.1 4,583,781 + Pf1N1B4_4141 0.18 -1.4 4,583,845 - Pf1N1B4_4141 0.21 +0.9 4,583,868 + Pf1N1B4_4141 0.22 -0.2 4,583,868 + Pf1N1B4_4141 0.22 +0.6 4,583,868 + Pf1N1B4_4141 0.22 +0.1 4,583,868 + Pf1N1B4_4141 0.22 +0.1 4,583,868 + Pf1N1B4_4141 0.22 -0.4 4,583,869 - -2.0 4,583,869 - Pf1N1B4_4141 0.22 +0.1 4,583,869 - Pf1N1B4_4141 0.22 -0.4 4,583,869 - Pf1N1B4_4141 0.22 -1.2 4,583,870 + Pf1N1B4_4141 0.22 +0.1 4,583,877 - Pf1N1B4_4141 0.22 -2.0 4,584,008 + Pf1N1B4_4141 0.27 -1.4 4,584,009 - Pf1N1B4_4141 0.27 -0.4 4,584,009 - Pf1N1B4_4141 0.27 +0.6 4,584,009 - Pf1N1B4_4141 0.27 +0.9 4,584,021 + Pf1N1B4_4141 0.28 -0.0
Or see this region's nucleotide sequence