Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4117 and Pf1N1B4_4118 are separated by 748 nucleotides Pf1N1B4_4118 and Pf1N1B4_4119 are separated by 486 nucleotides Pf1N1B4_4119 and Pf1N1B4_4120 are separated by 67 nucleotides
Pf1N1B4_4117 - hypothetical protein, at 4,559,628 to 4,559,915
_4117
Pf1N1B4_4118 - Cyn operon transcriptional activator, at 4,560,664 to 4,561,593
_4118
Pf1N1B4_4119 - dioxygenase, at 4,562,080 to 4,562,391
_4119
Pf1N1B4_4120 - hypothetical protein, at 4,562,459 to 4,562,647
_4120
Position (kb)
4560
4561
4562 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4559.691 kb on - strand, within Pf1N1B4_4117 at 4559.932 kb on + strand at 4559.932 kb on + strand at 4559.932 kb on + strand at 4560.044 kb on - strand at 4560.076 kb on + strand at 4560.080 kb on + strand at 4560.080 kb on + strand at 4560.081 kb on - strand at 4560.090 kb on + strand at 4560.091 kb on - strand at 4560.149 kb on + strand at 4560.247 kb on - strand at 4560.247 kb on - strand at 4560.304 kb on - strand at 4560.352 kb on - strand at 4560.352 kb on - strand at 4560.355 kb on + strand at 4560.356 kb on - strand at 4560.356 kb on - strand at 4560.508 kb on + strand at 4560.509 kb on - strand at 4560.509 kb on - strand at 4560.509 kb on - strand at 4560.512 kb on + strand at 4560.512 kb on + strand at 4560.513 kb on - strand at 4560.513 kb on - strand at 4560.575 kb on + strand at 4560.575 kb on + strand at 4560.575 kb on + strand at 4560.575 kb on + strand at 4560.576 kb on - strand at 4560.576 kb on - strand at 4560.675 kb on + strand at 4560.675 kb on + strand at 4560.676 kb on - strand at 4560.676 kb on - strand at 4560.676 kb on - strand at 4560.689 kb on + strand at 4560.689 kb on + strand at 4560.689 kb on + strand at 4560.690 kb on - strand at 4560.690 kb on - strand at 4560.690 kb on - strand at 4560.690 kb on - strand at 4560.741 kb on + strand at 4560.741 kb on + strand at 4560.742 kb on - strand at 4560.742 kb on - strand at 4560.877 kb on + strand at 4560.878 kb on - strand, within Pf1N1B4_4118 at 4561.009 kb on + strand, within Pf1N1B4_4118 at 4561.151 kb on - strand, within Pf1N1B4_4118 at 4561.151 kb on - strand, within Pf1N1B4_4118 at 4561.159 kb on + strand at 4561.159 kb on + strand, within Pf1N1B4_4118 at 4561.165 kb on + strand, within Pf1N1B4_4118 at 4561.447 kb on + strand, within Pf1N1B4_4118 at 4561.592 kb on - strand at 4561.684 kb on - strand at 4561.742 kb on + strand at 4561.837 kb on + strand at 4561.838 kb on - strand at 4561.838 kb on - strand at 4561.838 kb on - strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.892 kb on + strand at 4561.893 kb on - strand at 4561.893 kb on - strand at 4561.922 kb on + strand at 4561.975 kb on + strand at 4562.079 kb on + strand at 4562.084 kb on + strand at 4562.146 kb on + strand, within Pf1N1B4_4119 at 4562.147 kb on - strand, within Pf1N1B4_4119 at 4562.185 kb on + strand, within Pf1N1B4_4119 at 4562.186 kb on - strand, within Pf1N1B4_4119 at 4562.186 kb on - strand, within Pf1N1B4_4119 at 4562.279 kb on + strand, within Pf1N1B4_4119 at 4562.279 kb on + strand, within Pf1N1B4_4119 at 4562.417 kb on + strand at 4562.587 kb on + strand, within Pf1N1B4_4120 at 4562.588 kb on - strand, within Pf1N1B4_4120
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,559,691 - Pf1N1B4_4117 0.22 +1.4 4,559,932 + +0.0 4,559,932 + +0.9 4,559,932 + -0.4 4,560,044 - +0.2 4,560,076 + -0.0 4,560,080 + -0.6 4,560,080 + -1.4 4,560,081 - +1.2 4,560,090 + +0.6 4,560,091 - +0.6 4,560,149 + +0.6 4,560,247 - -0.4 4,560,247 - -0.1 4,560,304 - -1.0 4,560,352 - +0.1 4,560,352 - -1.0 4,560,355 + -1.0 4,560,356 - +0.6 4,560,356 - +0.1 4,560,508 + -0.6 4,560,509 - +2.2 4,560,509 - -2.0 4,560,509 - -1.6 4,560,512 + +0.2 4,560,512 + -0.8 4,560,513 - -0.8 4,560,513 - +2.2 4,560,575 + -0.2 4,560,575 + -0.4 4,560,575 + +1.2 4,560,575 + +0.6 4,560,576 - -1.4 4,560,576 - +0.8 4,560,675 + -1.6 4,560,675 + -1.0 4,560,676 - -2.4 4,560,676 - -1.0 4,560,676 - -1.4 4,560,689 + -0.8 4,560,689 + -1.0 4,560,689 + +0.9 4,560,690 - +0.3 4,560,690 - +0.3 4,560,690 - -0.4 4,560,690 - +1.9 4,560,741 + -1.7 4,560,741 + -0.4 4,560,742 - -0.4 4,560,742 - -0.4 4,560,877 + -0.4 4,560,878 - Pf1N1B4_4118 0.23 +0.6 4,561,009 + Pf1N1B4_4118 0.37 -0.4 4,561,151 - Pf1N1B4_4118 0.52 +1.2 4,561,151 - Pf1N1B4_4118 0.52 +0.2 4,561,159 + +1.6 4,561,159 + Pf1N1B4_4118 0.53 -2.0 4,561,165 + Pf1N1B4_4118 0.54 -0.0 4,561,447 + Pf1N1B4_4118 0.84 +0.1 4,561,592 - -0.4 4,561,684 - -1.0 4,561,742 + -0.4 4,561,837 + +1.2 4,561,838 - -0.1 4,561,838 - -0.4 4,561,838 - -1.2 4,561,892 + -2.0 4,561,892 + +0.3 4,561,892 + +1.2 4,561,892 + +0.6 4,561,893 - +0.5 4,561,893 - +0.6 4,561,922 + +1.6 4,561,975 + +1.2 4,562,079 + +0.5 4,562,084 + +0.3 4,562,146 + Pf1N1B4_4119 0.21 +0.6 4,562,147 - Pf1N1B4_4119 0.21 -0.8 4,562,185 + Pf1N1B4_4119 0.34 +3.0 4,562,186 - Pf1N1B4_4119 0.34 -1.4 4,562,186 - Pf1N1B4_4119 0.34 -0.2 4,562,279 + Pf1N1B4_4119 0.64 -0.8 4,562,279 + Pf1N1B4_4119 0.64 -1.4 4,562,417 + -0.8 4,562,587 + Pf1N1B4_4120 0.68 -0.4 4,562,588 - Pf1N1B4_4120 0.68 +1.6
Or see this region's nucleotide sequence