Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4110

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4108 and Pf1N1B4_4109 are separated by 133 nucleotidesPf1N1B4_4109 and Pf1N1B4_4110 are separated by 130 nucleotidesPf1N1B4_4110 and Pf1N1B4_4111 are separated by 173 nucleotidesPf1N1B4_4111 and Pf1N1B4_4112 are separated by 141 nucleotidesPf1N1B4_4112 and Pf1N1B4_4113 overlap by 4 nucleotides Pf1N1B4_4108 - Hydrolase, alpha/beta fold family, at 4,554,921 to 4,555,718 _4108 Pf1N1B4_4109 - Cytochrome c2, at 4,555,852 to 4,556,241 _4109 Pf1N1B4_4110 - hypothetical protein, at 4,556,372 to 4,556,707 _4110 Pf1N1B4_4111 - Panthothenate synthetase, at 4,556,881 to 4,557,171 _4111 Pf1N1B4_4112 - hypothetical protein, at 4,557,313 to 4,557,564 _4112 Pf1N1B4_4113 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 4,557,561 to 4,558,040 _4113 Position (kb) 4556 4557Strain fitness (log2 ratio) -2 -1 0 1 2at 4555.385 kb on - strand, within Pf1N1B4_4108at 4555.385 kb on - strand, within Pf1N1B4_4108at 4555.450 kb on + strand, within Pf1N1B4_4108at 4555.451 kb on - strand, within Pf1N1B4_4108at 4555.451 kb on - strand, within Pf1N1B4_4108at 4555.451 kb on - strandat 4555.451 kb on - strand, within Pf1N1B4_4108at 4555.697 kb on - strandat 4555.761 kb on + strandat 4555.761 kb on + strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.767 kb on - strandat 4555.767 kb on - strandat 4555.786 kb on - strandat 4555.952 kb on + strand, within Pf1N1B4_4109at 4555.952 kb on + strand, within Pf1N1B4_4109at 4555.953 kb on - strand, within Pf1N1B4_4109at 4555.953 kb on - strandat 4555.953 kb on - strandat 4555.962 kb on + strand, within Pf1N1B4_4109at 4555.963 kb on - strand, within Pf1N1B4_4109at 4555.985 kb on + strand, within Pf1N1B4_4109at 4555.986 kb on - strand, within Pf1N1B4_4109at 4556.062 kb on + strandat 4556.063 kb on - strand, within Pf1N1B4_4109at 4556.068 kb on + strand, within Pf1N1B4_4109at 4556.119 kb on - strand, within Pf1N1B4_4109at 4556.125 kb on + strand, within Pf1N1B4_4109at 4556.147 kb on + strand, within Pf1N1B4_4109at 4556.216 kb on - strandat 4556.251 kb on + strandat 4556.378 kb on + strandat 4556.379 kb on - strandat 4556.379 kb on - strandat 4556.379 kb on - strandat 4556.379 kb on - strandat 4556.379 kb on - strandat 4556.474 kb on + strand, within Pf1N1B4_4110at 4556.474 kb on + strand, within Pf1N1B4_4110at 4556.475 kb on - strand, within Pf1N1B4_4110at 4556.475 kb on - strand, within Pf1N1B4_4110at 4557.020 kb on - strand, within Pf1N1B4_4111at 4557.020 kb on - strand, within Pf1N1B4_4111at 4557.020 kb on - strand, within Pf1N1B4_4111at 4557.020 kb on - strand, within Pf1N1B4_4111at 4557.020 kb on - strand, within Pf1N1B4_4111at 4557.188 kb on - strandat 4557.194 kb on + strandat 4557.231 kb on + strandat 4557.231 kb on + strandat 4557.231 kb on + strandat 4557.232 kb on - strandat 4557.291 kb on - strandat 4557.328 kb on + strandat 4557.329 kb on - strandat 4557.423 kb on + strand, within Pf1N1B4_4112at 4557.425 kb on + strand, within Pf1N1B4_4112at 4557.425 kb on + strandat 4557.425 kb on + strand, within Pf1N1B4_4112at 4557.425 kb on + strand, within Pf1N1B4_4112at 4557.425 kb on + strand, within Pf1N1B4_4112at 4557.425 kb on + strand, within Pf1N1B4_4112at 4557.425 kb on + strand, within Pf1N1B4_4112at 4557.425 kb on + strand, within Pf1N1B4_4112at 4557.425 kb on + strand, within Pf1N1B4_4112at 4557.426 kb on - strand, within Pf1N1B4_4112at 4557.426 kb on - strand, within Pf1N1B4_4112at 4557.426 kb on - strand, within Pf1N1B4_4112at 4557.426 kb on - strand, within Pf1N1B4_4112at 4557.426 kb on - strand, within Pf1N1B4_4112at 4557.472 kb on - strand, within Pf1N1B4_4112at 4557.472 kb on - strand, within Pf1N1B4_4112at 4557.472 kb on - strand, within Pf1N1B4_4112at 4557.549 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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4,555,385 - Pf1N1B4_4108 0.58 +1.2
4,555,385 - Pf1N1B4_4108 0.58 -0.3
4,555,450 + Pf1N1B4_4108 0.66 -1.0
4,555,451 - Pf1N1B4_4108 0.66 -0.4
4,555,451 - Pf1N1B4_4108 0.66 -0.4
4,555,451 - -2.0
4,555,451 - Pf1N1B4_4108 0.66 -0.4
4,555,697 - -0.4
4,555,761 + +0.8
4,555,761 + +1.2
4,555,762 - -1.2
4,555,762 - -0.4
4,555,762 - -0.2
4,555,762 - -0.4
4,555,762 - +0.4
4,555,762 - +0.6
4,555,762 - -2.0
4,555,762 - -0.3
4,555,762 - +0.2
4,555,767 - -1.4
4,555,767 - -0.9
4,555,786 - -0.6
4,555,952 + Pf1N1B4_4109 0.26 +0.2
4,555,952 + Pf1N1B4_4109 0.26 +1.2
4,555,953 - Pf1N1B4_4109 0.26 +2.4
4,555,953 - +0.6
4,555,953 - -0.4
4,555,962 + Pf1N1B4_4109 0.28 +0.6
4,555,963 - Pf1N1B4_4109 0.28 -0.4
4,555,985 + Pf1N1B4_4109 0.34 -0.2
4,555,986 - Pf1N1B4_4109 0.34 -0.8
4,556,062 + +0.6
4,556,063 - Pf1N1B4_4109 0.54 -1.2
4,556,068 + Pf1N1B4_4109 0.55 -0.1
4,556,119 - Pf1N1B4_4109 0.68 -2.0
4,556,125 + Pf1N1B4_4109 0.70 -0.4
4,556,147 + Pf1N1B4_4109 0.76 -1.2
4,556,216 - -0.0
4,556,251 + -0.4
4,556,378 + -1.3
4,556,379 - +1.2
4,556,379 - -0.7
4,556,379 - +1.2
4,556,379 - +0.2
4,556,379 - -1.0
4,556,474 + Pf1N1B4_4110 0.30 -2.0
4,556,474 + Pf1N1B4_4110 0.30 -0.4
4,556,475 - Pf1N1B4_4110 0.31 -0.8
4,556,475 - Pf1N1B4_4110 0.31 -1.8
4,557,020 - Pf1N1B4_4111 0.48 +0.4
4,557,020 - Pf1N1B4_4111 0.48 -0.3
4,557,020 - Pf1N1B4_4111 0.48 -1.4
4,557,020 - Pf1N1B4_4111 0.48 +0.1
4,557,020 - Pf1N1B4_4111 0.48 -1.2
4,557,188 - +0.8
4,557,194 + -0.4
4,557,231 + +1.6
4,557,231 + -2.4
4,557,231 + -0.5
4,557,232 - -0.4
4,557,291 - +0.9
4,557,328 + -2.4
4,557,329 - -0.1
4,557,423 + Pf1N1B4_4112 0.44 -1.4
4,557,425 + Pf1N1B4_4112 0.44 -1.8
4,557,425 + -0.4
4,557,425 + Pf1N1B4_4112 0.44 -1.1
4,557,425 + Pf1N1B4_4112 0.44 -0.2
4,557,425 + Pf1N1B4_4112 0.44 -2.4
4,557,425 + Pf1N1B4_4112 0.44 -0.4
4,557,425 + Pf1N1B4_4112 0.44 -0.4
4,557,425 + Pf1N1B4_4112 0.44 -0.7
4,557,425 + Pf1N1B4_4112 0.44 -1.4
4,557,426 - Pf1N1B4_4112 0.45 +0.2
4,557,426 - Pf1N1B4_4112 0.45 -1.8
4,557,426 - Pf1N1B4_4112 0.45 -1.4
4,557,426 - Pf1N1B4_4112 0.45 -0.7
4,557,426 - Pf1N1B4_4112 0.45 -0.7
4,557,472 - Pf1N1B4_4112 0.63 +0.2
4,557,472 - Pf1N1B4_4112 0.63 -0.4
4,557,472 - Pf1N1B4_4112 0.63 -2.3
4,557,549 - -1.0

Or see this region's nucleotide sequence