Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4065

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4064 and Pf1N1B4_4065 are separated by 0 nucleotidesPf1N1B4_4065 and Pf1N1B4_4066 are separated by 139 nucleotides Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289 _4064 Pf1N1B4_4065 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 4,519,290 to 4,520,684 _4065 Pf1N1B4_4066 - Glutathione S-transferase, omega (EC 2.5.1.18), at 4,520,824 to 4,521,828 _4066 Position (kb) 4519 4520 4521Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4519.287 kb on + strandat 4519.296 kb on + strandat 4519.296 kb on + strandat 4519.296 kb on + strandat 4519.297 kb on - strandat 4519.333 kb on + strandat 4519.333 kb on + strandat 4519.408 kb on + strandat 4519.408 kb on + strandat 4519.741 kb on - strand, within Pf1N1B4_4065at 4519.834 kb on + strand, within Pf1N1B4_4065at 4519.834 kb on + strand, within Pf1N1B4_4065at 4519.834 kb on + strandat 4519.834 kb on + strand, within Pf1N1B4_4065at 4519.834 kb on + strand, within Pf1N1B4_4065at 4519.834 kb on + strand, within Pf1N1B4_4065at 4519.834 kb on + strand, within Pf1N1B4_4065at 4519.835 kb on - strand, within Pf1N1B4_4065at 4519.835 kb on - strand, within Pf1N1B4_4065at 4519.835 kb on - strand, within Pf1N1B4_4065at 4519.835 kb on - strand, within Pf1N1B4_4065at 4519.835 kb on - strand, within Pf1N1B4_4065at 4520.028 kb on + strand, within Pf1N1B4_4065at 4520.028 kb on + strand, within Pf1N1B4_4065at 4520.028 kb on + strand, within Pf1N1B4_4065at 4520.028 kb on + strand, within Pf1N1B4_4065at 4520.028 kb on + strand, within Pf1N1B4_4065at 4520.028 kb on + strand, within Pf1N1B4_4065at 4520.029 kb on - strand, within Pf1N1B4_4065at 4520.029 kb on - strand, within Pf1N1B4_4065at 4520.088 kb on + strand, within Pf1N1B4_4065at 4520.088 kb on + strand, within Pf1N1B4_4065at 4520.088 kb on + strand, within Pf1N1B4_4065at 4520.088 kb on + strand, within Pf1N1B4_4065at 4520.088 kb on + strand, within Pf1N1B4_4065at 4520.204 kb on + strand, within Pf1N1B4_4065at 4520.205 kb on - strand, within Pf1N1B4_4065at 4520.304 kb on + strand, within Pf1N1B4_4065at 4520.433 kb on + strand, within Pf1N1B4_4065at 4520.433 kb on + strand, within Pf1N1B4_4065at 4520.433 kb on + strand, within Pf1N1B4_4065at 4520.433 kb on + strand, within Pf1N1B4_4065at 4520.433 kb on + strand, within Pf1N1B4_4065at 4520.433 kb on + strand, within Pf1N1B4_4065at 4520.434 kb on - strand, within Pf1N1B4_4065at 4520.596 kb on + strandat 4520.596 kb on + strandat 4520.596 kb on + strandat 4520.596 kb on + strandat 4520.596 kb on + strandat 4520.596 kb on + strandat 4520.596 kb on + strandat 4520.596 kb on + strandat 4520.597 kb on - strandat 4520.597 kb on - strandat 4520.597 kb on - strandat 4520.727 kb on - strandat 4520.949 kb on - strand, within Pf1N1B4_4066at 4520.949 kb on - strand, within Pf1N1B4_4066at 4520.951 kb on + strand, within Pf1N1B4_4066at 4520.952 kb on - strand, within Pf1N1B4_4066at 4520.952 kb on - strand, within Pf1N1B4_4066at 4520.952 kb on - strand, within Pf1N1B4_4066at 4520.952 kb on - strand, within Pf1N1B4_4066at 4521.006 kb on - strand, within Pf1N1B4_4066at 4521.006 kb on - strand, within Pf1N1B4_4066at 4521.080 kb on + strand, within Pf1N1B4_4066at 4521.080 kb on + strand, within Pf1N1B4_4066at 4521.080 kb on + strand, within Pf1N1B4_4066at 4521.081 kb on - strand, within Pf1N1B4_4066at 4521.200 kb on - strandat 4521.221 kb on + strand, within Pf1N1B4_4066at 4521.222 kb on - strand, within Pf1N1B4_4066at 4521.222 kb on - strand, within Pf1N1B4_4066at 4521.479 kb on + strand, within Pf1N1B4_4066at 4521.662 kb on + strandat 4521.663 kb on - strand, within Pf1N1B4_4066

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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4,519,287 + -2.2
4,519,296 + -0.7
4,519,296 + -3.7
4,519,296 + -2.7
4,519,297 - -1.4
4,519,333 + -3.0
4,519,333 + -3.2
4,519,408 + -2.4
4,519,408 + -1.4
4,519,741 - Pf1N1B4_4065 0.32 -0.4
4,519,834 + Pf1N1B4_4065 0.39 -2.0
4,519,834 + Pf1N1B4_4065 0.39 -0.4
4,519,834 + -0.4
4,519,834 + Pf1N1B4_4065 0.39 -1.4
4,519,834 + Pf1N1B4_4065 0.39 -2.0
4,519,834 + Pf1N1B4_4065 0.39 -1.4
4,519,834 + Pf1N1B4_4065 0.39 -3.0
4,519,835 - Pf1N1B4_4065 0.39 -2.4
4,519,835 - Pf1N1B4_4065 0.39 -0.4
4,519,835 - Pf1N1B4_4065 0.39 -0.4
4,519,835 - Pf1N1B4_4065 0.39 -2.4
4,519,835 - Pf1N1B4_4065 0.39 -2.7
4,520,028 + Pf1N1B4_4065 0.53 +0.2
4,520,028 + Pf1N1B4_4065 0.53 -3.0
4,520,028 + Pf1N1B4_4065 0.53 -1.7
4,520,028 + Pf1N1B4_4065 0.53 -2.0
4,520,028 + Pf1N1B4_4065 0.53 -1.4
4,520,028 + Pf1N1B4_4065 0.53 -2.0
4,520,029 - Pf1N1B4_4065 0.53 -1.4
4,520,029 - Pf1N1B4_4065 0.53 -2.4
4,520,088 + Pf1N1B4_4065 0.57 -2.7
4,520,088 + Pf1N1B4_4065 0.57 -2.0
4,520,088 + Pf1N1B4_4065 0.57 -3.2
4,520,088 + Pf1N1B4_4065 0.57 -0.9
4,520,088 + Pf1N1B4_4065 0.57 -2.4
4,520,204 + Pf1N1B4_4065 0.66 -4.4
4,520,205 - Pf1N1B4_4065 0.66 -1.4
4,520,304 + Pf1N1B4_4065 0.73 -0.4
4,520,433 + Pf1N1B4_4065 0.82 -1.0
4,520,433 + Pf1N1B4_4065 0.82 -3.2
4,520,433 + Pf1N1B4_4065 0.82 -2.4
4,520,433 + Pf1N1B4_4065 0.82 -2.4
4,520,433 + Pf1N1B4_4065 0.82 -2.4
4,520,433 + Pf1N1B4_4065 0.82 -2.0
4,520,434 - Pf1N1B4_4065 0.82 -3.0
4,520,596 + -3.2
4,520,596 + -1.4
4,520,596 + -2.4
4,520,596 + -3.7
4,520,596 + -3.2
4,520,596 + -4.0
4,520,596 + -2.4
4,520,596 + -2.0
4,520,597 - -0.4
4,520,597 - -0.4
4,520,597 - -1.4
4,520,727 - -1.0
4,520,949 - Pf1N1B4_4066 0.12 +0.6
4,520,949 - Pf1N1B4_4066 0.12 +1.6
4,520,951 + Pf1N1B4_4066 0.13 -1.7
4,520,952 - Pf1N1B4_4066 0.13 -0.4
4,520,952 - Pf1N1B4_4066 0.13 -0.6
4,520,952 - Pf1N1B4_4066 0.13 -0.8
4,520,952 - Pf1N1B4_4066 0.13 -1.4
4,521,006 - Pf1N1B4_4066 0.18 +1.2
4,521,006 - Pf1N1B4_4066 0.18 +0.5
4,521,080 + Pf1N1B4_4066 0.25 -1.1
4,521,080 + Pf1N1B4_4066 0.25 -0.2
4,521,080 + Pf1N1B4_4066 0.25 -1.4
4,521,081 - Pf1N1B4_4066 0.26 -0.5
4,521,200 - -0.4
4,521,221 + Pf1N1B4_4066 0.40 +0.3
4,521,222 - Pf1N1B4_4066 0.40 +0.4
4,521,222 - Pf1N1B4_4066 0.40 -1.8
4,521,479 + Pf1N1B4_4066 0.65 +0.0
4,521,662 + -0.4
4,521,663 - Pf1N1B4_4066 0.83 -0.1

Or see this region's nucleotide sequence