Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4064 and Pf1N1B4_4065 are separated by 0 nucleotides Pf1N1B4_4065 and Pf1N1B4_4066 are separated by 139 nucleotides
Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289
_4064
Pf1N1B4_4065 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 4,519,290 to 4,520,684
_4065
Pf1N1B4_4066 - Glutathione S-transferase, omega (EC 2.5.1.18), at 4,520,824 to 4,521,828
_4066
Position (kb)
4519
4520
4521 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4519.287 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.297 kb on - strand at 4519.333 kb on + strand at 4519.333 kb on + strand at 4519.408 kb on + strand at 4519.408 kb on + strand at 4519.741 kb on - strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.204 kb on + strand, within Pf1N1B4_4065 at 4520.205 kb on - strand, within Pf1N1B4_4065 at 4520.304 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.434 kb on - strand, within Pf1N1B4_4065 at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.597 kb on - strand at 4520.597 kb on - strand at 4520.597 kb on - strand at 4520.727 kb on - strand at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.951 kb on + strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.081 kb on - strand, within Pf1N1B4_4066 at 4521.200 kb on - strand at 4521.221 kb on + strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.479 kb on + strand, within Pf1N1B4_4066 at 4521.662 kb on + strand at 4521.663 kb on - strand, within Pf1N1B4_4066
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,519,287 + -2.2 4,519,296 + -0.7 4,519,296 + -3.7 4,519,296 + -2.7 4,519,297 - -1.4 4,519,333 + -3.0 4,519,333 + -3.2 4,519,408 + -2.4 4,519,408 + -1.4 4,519,741 - Pf1N1B4_4065 0.32 -0.4 4,519,834 + Pf1N1B4_4065 0.39 -2.0 4,519,834 + Pf1N1B4_4065 0.39 -0.4 4,519,834 + -0.4 4,519,834 + Pf1N1B4_4065 0.39 -1.4 4,519,834 + Pf1N1B4_4065 0.39 -2.0 4,519,834 + Pf1N1B4_4065 0.39 -1.4 4,519,834 + Pf1N1B4_4065 0.39 -3.0 4,519,835 - Pf1N1B4_4065 0.39 -2.4 4,519,835 - Pf1N1B4_4065 0.39 -0.4 4,519,835 - Pf1N1B4_4065 0.39 -0.4 4,519,835 - Pf1N1B4_4065 0.39 -2.4 4,519,835 - Pf1N1B4_4065 0.39 -2.7 4,520,028 + Pf1N1B4_4065 0.53 +0.2 4,520,028 + Pf1N1B4_4065 0.53 -3.0 4,520,028 + Pf1N1B4_4065 0.53 -1.7 4,520,028 + Pf1N1B4_4065 0.53 -2.0 4,520,028 + Pf1N1B4_4065 0.53 -1.4 4,520,028 + Pf1N1B4_4065 0.53 -2.0 4,520,029 - Pf1N1B4_4065 0.53 -1.4 4,520,029 - Pf1N1B4_4065 0.53 -2.4 4,520,088 + Pf1N1B4_4065 0.57 -2.7 4,520,088 + Pf1N1B4_4065 0.57 -2.0 4,520,088 + Pf1N1B4_4065 0.57 -3.2 4,520,088 + Pf1N1B4_4065 0.57 -0.9 4,520,088 + Pf1N1B4_4065 0.57 -2.4 4,520,204 + Pf1N1B4_4065 0.66 -4.4 4,520,205 - Pf1N1B4_4065 0.66 -1.4 4,520,304 + Pf1N1B4_4065 0.73 -0.4 4,520,433 + Pf1N1B4_4065 0.82 -1.0 4,520,433 + Pf1N1B4_4065 0.82 -3.2 4,520,433 + Pf1N1B4_4065 0.82 -2.4 4,520,433 + Pf1N1B4_4065 0.82 -2.4 4,520,433 + Pf1N1B4_4065 0.82 -2.4 4,520,433 + Pf1N1B4_4065 0.82 -2.0 4,520,434 - Pf1N1B4_4065 0.82 -3.0 4,520,596 + -3.2 4,520,596 + -1.4 4,520,596 + -2.4 4,520,596 + -3.7 4,520,596 + -3.2 4,520,596 + -4.0 4,520,596 + -2.4 4,520,596 + -2.0 4,520,597 - -0.4 4,520,597 - -0.4 4,520,597 - -1.4 4,520,727 - -1.0 4,520,949 - Pf1N1B4_4066 0.12 +0.6 4,520,949 - Pf1N1B4_4066 0.12 +1.6 4,520,951 + Pf1N1B4_4066 0.13 -1.7 4,520,952 - Pf1N1B4_4066 0.13 -0.4 4,520,952 - Pf1N1B4_4066 0.13 -0.6 4,520,952 - Pf1N1B4_4066 0.13 -0.8 4,520,952 - Pf1N1B4_4066 0.13 -1.4 4,521,006 - Pf1N1B4_4066 0.18 +1.2 4,521,006 - Pf1N1B4_4066 0.18 +0.5 4,521,080 + Pf1N1B4_4066 0.25 -1.1 4,521,080 + Pf1N1B4_4066 0.25 -0.2 4,521,080 + Pf1N1B4_4066 0.25 -1.4 4,521,081 - Pf1N1B4_4066 0.26 -0.5 4,521,200 - -0.4 4,521,221 + Pf1N1B4_4066 0.40 +0.3 4,521,222 - Pf1N1B4_4066 0.40 +0.4 4,521,222 - Pf1N1B4_4066 0.40 -1.8 4,521,479 + Pf1N1B4_4066 0.65 +0.0 4,521,662 + -0.4 4,521,663 - Pf1N1B4_4066 0.83 -0.1
Or see this region's nucleotide sequence