Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4052 and Pf1N1B4_4053 are separated by 61 nucleotides Pf1N1B4_4053 and Pf1N1B4_4054 are separated by 341 nucleotides Pf1N1B4_4054 and Pf1N1B4_4055 are separated by 30 nucleotides Pf1N1B4_4055 and Pf1N1B4_4056 are separated by 259 nucleotides Pf1N1B4_4056 and Pf1N1B4_4057 are separated by 225 nucleotides
Pf1N1B4_4052 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,505,027 to 4,506,283
_4052
Pf1N1B4_4053 - Cold shock protein CspD, at 4,506,345 to 4,506,626
_4053
Pf1N1B4_4054 - ATP-dependent Clp protease adaptor protein ClpS, at 4,506,968 to 4,507,213
_4054
Pf1N1B4_4055 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 4,507,244 to 4,509,514
_4055
Pf1N1B4_4056 - Translation initiation factor 1, at 4,509,774 to 4,509,992
_4056
Pf1N1B4_4057 - Arginine-tRNA-protein transferase (EC 2.3.2.8), at 4,510,218 to 4,510,925
_4057
Position (kb)
4507
4508
4509
4510 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4506.281 kb on + strand at 4506.281 kb on + strand at 4506.281 kb on + strand at 4506.282 kb on - strand at 4506.487 kb on + strand, within Pf1N1B4_4053 at 4506.488 kb on - strand, within Pf1N1B4_4053 at 4506.512 kb on - strand, within Pf1N1B4_4053 at 4506.555 kb on + strand, within Pf1N1B4_4053 at 4506.555 kb on + strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.663 kb on + strand at 4506.664 kb on - strand at 4506.686 kb on + strand at 4506.687 kb on - strand at 4506.687 kb on - strand at 4506.698 kb on + strand at 4506.739 kb on - strand at 4506.821 kb on + strand at 4506.821 kb on + strand at 4506.822 kb on - strand at 4506.822 kb on - strand at 4506.876 kb on + strand at 4506.903 kb on + strand at 4506.903 kb on + strand at 4506.943 kb on + strand at 4506.954 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.972 kb on - strand at 4506.972 kb on - strand at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.999 kb on - strand, within Pf1N1B4_4054 at 4506.999 kb on - strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.105 kb on - strand, within Pf1N1B4_4054 at 4507.160 kb on + strand, within Pf1N1B4_4054 at 4507.244 kb on - strand at 4507.503 kb on + strand, within Pf1N1B4_4055 at 4507.504 kb on - strand at 4507.527 kb on + strand, within Pf1N1B4_4055 at 4507.527 kb on + strand, within Pf1N1B4_4055 at 4507.527 kb on + strand, within Pf1N1B4_4055 at 4507.527 kb on + strand, within Pf1N1B4_4055 at 4507.528 kb on - strand, within Pf1N1B4_4055 at 4507.561 kb on + strand, within Pf1N1B4_4055 at 4507.652 kb on + strand, within Pf1N1B4_4055 at 4507.652 kb on + strand, within Pf1N1B4_4055 at 4507.652 kb on + strand, within Pf1N1B4_4055 at 4507.652 kb on + strand, within Pf1N1B4_4055 at 4507.652 kb on + strand, within Pf1N1B4_4055 at 4507.653 kb on - strand at 4507.653 kb on - strand, within Pf1N1B4_4055 at 4507.653 kb on - strand, within Pf1N1B4_4055 at 4507.714 kb on + strand, within Pf1N1B4_4055 at 4507.714 kb on + strand, within Pf1N1B4_4055 at 4507.769 kb on + strand, within Pf1N1B4_4055 at 4507.821 kb on + strand, within Pf1N1B4_4055 at 4507.991 kb on + strand, within Pf1N1B4_4055 at 4508.372 kb on + strand, within Pf1N1B4_4055 at 4508.515 kb on + strand, within Pf1N1B4_4055 at 4508.606 kb on - strand, within Pf1N1B4_4055 at 4508.634 kb on + strand, within Pf1N1B4_4055 at 4508.634 kb on + strand, within Pf1N1B4_4055 at 4508.635 kb on - strand, within Pf1N1B4_4055 at 4508.825 kb on + strand, within Pf1N1B4_4055 at 4508.825 kb on + strand, within Pf1N1B4_4055 at 4508.825 kb on + strand, within Pf1N1B4_4055 at 4508.825 kb on + strand, within Pf1N1B4_4055 at 4508.825 kb on + strand, within Pf1N1B4_4055 at 4508.826 kb on - strand, within Pf1N1B4_4055 at 4508.826 kb on - strand, within Pf1N1B4_4055 at 4508.873 kb on + strand, within Pf1N1B4_4055 at 4508.873 kb on + strand, within Pf1N1B4_4055 at 4509.116 kb on + strand, within Pf1N1B4_4055 at 4509.338 kb on + strand at 4509.450 kb on + strand at 4509.450 kb on + strand at 4509.450 kb on + strand at 4509.450 kb on + strand at 4509.718 kb on + strand at 4509.719 kb on - strand at 4509.719 kb on - strand at 4510.112 kb on - strand at 4510.112 kb on - strand at 4510.112 kb on - strand at 4510.112 kb on - strand at 4510.200 kb on + strand at 4510.201 kb on - strand at 4510.201 kb on - strand at 4510.280 kb on - strand at 4510.280 kb on - strand at 4510.319 kb on - strand, within Pf1N1B4_4057 at 4510.319 kb on - strand, within Pf1N1B4_4057 at 4510.408 kb on + strand, within Pf1N1B4_4057 at 4510.417 kb on + strand, within Pf1N1B4_4057 at 4510.418 kb on - strand, within Pf1N1B4_4057 at 4510.493 kb on - strand, within Pf1N1B4_4057 at 4510.493 kb on - strand, within Pf1N1B4_4057 at 4510.493 kb on - strand, within Pf1N1B4_4057
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,506,281 + -0.4 4,506,281 + -2.4 4,506,281 + +1.2 4,506,282 - +2.0 4,506,487 + Pf1N1B4_4053 0.50 +0.2 4,506,488 - Pf1N1B4_4053 0.51 -2.0 4,506,512 - Pf1N1B4_4053 0.59 -2.0 4,506,555 + Pf1N1B4_4053 0.74 -1.1 4,506,555 + Pf1N1B4_4053 0.74 +0.4 4,506,556 - Pf1N1B4_4053 0.75 +0.2 4,506,556 - Pf1N1B4_4053 0.75 -0.6 4,506,556 - Pf1N1B4_4053 0.75 -1.0 4,506,663 + -0.4 4,506,664 - +0.9 4,506,686 + +0.4 4,506,687 - -1.0 4,506,687 - -1.4 4,506,698 + +1.2 4,506,739 - -1.4 4,506,821 + -3.0 4,506,821 + +0.6 4,506,822 - +0.0 4,506,822 - -2.2 4,506,876 + -3.0 4,506,903 + -0.4 4,506,903 + -0.4 4,506,943 + -0.5 4,506,954 + +0.9 4,506,971 + +0.2 4,506,971 + -1.0 4,506,971 + +0.4 4,506,971 + -0.0 4,506,971 + -0.4 4,506,971 + +0.6 4,506,971 + +0.5 4,506,971 + +0.4 4,506,971 + -1.6 4,506,971 + -0.0 4,506,971 + +1.0 4,506,971 + -1.0 4,506,972 - -0.4 4,506,972 - -2.0 4,506,998 + Pf1N1B4_4054 0.12 +0.0 4,506,998 + Pf1N1B4_4054 0.12 -1.6 4,506,998 + Pf1N1B4_4054 0.12 -0.5 4,506,998 + Pf1N1B4_4054 0.12 +0.4 4,506,998 + Pf1N1B4_4054 0.12 +1.0 4,506,999 - Pf1N1B4_4054 0.13 +0.0 4,506,999 - Pf1N1B4_4054 0.13 -0.4 4,507,104 + Pf1N1B4_4054 0.55 -1.4 4,507,104 + Pf1N1B4_4054 0.55 +0.5 4,507,104 + Pf1N1B4_4054 0.55 -1.0 4,507,104 + Pf1N1B4_4054 0.55 -0.4 4,507,104 + Pf1N1B4_4054 0.55 -0.1 4,507,104 + Pf1N1B4_4054 0.55 -0.6 4,507,104 + Pf1N1B4_4054 0.55 -2.0 4,507,104 + Pf1N1B4_4054 0.55 -0.9 4,507,104 + Pf1N1B4_4054 0.55 -0.1 4,507,104 + Pf1N1B4_4054 0.55 +0.5 4,507,104 + Pf1N1B4_4054 0.55 +0.3 4,507,105 - Pf1N1B4_4054 0.56 +0.6 4,507,160 + Pf1N1B4_4054 0.78 +0.0 4,507,244 - -2.4 4,507,503 + Pf1N1B4_4055 0.11 -1.7 4,507,504 - -0.4 4,507,527 + Pf1N1B4_4055 0.12 -3.7 4,507,527 + Pf1N1B4_4055 0.12 +0.0 4,507,527 + Pf1N1B4_4055 0.12 -1.4 4,507,527 + Pf1N1B4_4055 0.12 -2.0 4,507,528 - Pf1N1B4_4055 0.13 -1.4 4,507,561 + Pf1N1B4_4055 0.14 -2.4 4,507,652 + Pf1N1B4_4055 0.18 -3.4 4,507,652 + Pf1N1B4_4055 0.18 -2.4 4,507,652 + Pf1N1B4_4055 0.18 -1.6 4,507,652 + Pf1N1B4_4055 0.18 -3.0 4,507,652 + Pf1N1B4_4055 0.18 -2.7 4,507,653 - -1.4 4,507,653 - Pf1N1B4_4055 0.18 -2.0 4,507,653 - Pf1N1B4_4055 0.18 -0.4 4,507,714 + Pf1N1B4_4055 0.21 -1.7 4,507,714 + Pf1N1B4_4055 0.21 -2.0 4,507,769 + Pf1N1B4_4055 0.23 -0.4 4,507,821 + Pf1N1B4_4055 0.25 -2.4 4,507,991 + Pf1N1B4_4055 0.33 -0.4 4,508,372 + Pf1N1B4_4055 0.50 -1.1 4,508,515 + Pf1N1B4_4055 0.56 -1.4 4,508,606 - Pf1N1B4_4055 0.60 -2.4 4,508,634 + Pf1N1B4_4055 0.61 -2.3 4,508,634 + Pf1N1B4_4055 0.61 -2.4 4,508,635 - Pf1N1B4_4055 0.61 -3.0 4,508,825 + Pf1N1B4_4055 0.70 -1.6 4,508,825 + Pf1N1B4_4055 0.70 -1.7 4,508,825 + Pf1N1B4_4055 0.70 +0.6 4,508,825 + Pf1N1B4_4055 0.70 -2.7 4,508,825 + Pf1N1B4_4055 0.70 -2.7 4,508,826 - Pf1N1B4_4055 0.70 -1.7 4,508,826 - Pf1N1B4_4055 0.70 -2.0 4,508,873 + Pf1N1B4_4055 0.72 -0.4 4,508,873 + Pf1N1B4_4055 0.72 -2.0 4,509,116 + Pf1N1B4_4055 0.82 -2.0 4,509,338 + -2.0 4,509,450 + +0.6 4,509,450 + -2.4 4,509,450 + -2.2 4,509,450 + -1.5 4,509,718 + -1.0 4,509,719 - -1.4 4,509,719 - +0.6 4,510,112 - +1.9 4,510,112 - +0.8 4,510,112 - -2.2 4,510,112 - -1.0 4,510,200 + -1.4 4,510,201 - -2.7 4,510,201 - +1.6 4,510,280 - +0.6 4,510,280 - -0.5 4,510,319 - Pf1N1B4_4057 0.14 -1.2 4,510,319 - Pf1N1B4_4057 0.14 +2.1 4,510,408 + Pf1N1B4_4057 0.27 -0.4 4,510,417 + Pf1N1B4_4057 0.28 -0.9 4,510,418 - Pf1N1B4_4057 0.28 +0.2 4,510,493 - Pf1N1B4_4057 0.39 -0.2 4,510,493 - Pf1N1B4_4057 0.39 -0.4 4,510,493 - Pf1N1B4_4057 0.39 -0.4
Or see this region's nucleotide sequence