Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4041 and Pf1N1B4_4042 are separated by 649 nucleotides Pf1N1B4_4042 and Pf1N1B4_4043 are separated by 498 nucleotides
Pf1N1B4_4041 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 4,492,779 to 4,493,192
_4041
Pf1N1B4_4042 - Isocitrate lyase (EC 4.1.3.1) (from data) , at 4,493,842 to 4,495,167
_4042
Pf1N1B4_4043 - Type II and III secretion system family protein, at 4,495,666 to 4,496,427
_4043
Position (kb)
4493
4494
4495
4496 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.879 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.284 kb on + strand at 4493.326 kb on - strand at 4493.329 kb on + strand at 4493.330 kb on - strand at 4493.345 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.425 kb on - strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.551 kb on + strand at 4493.780 kb on + strand at 4493.781 kb on - strand at 4493.781 kb on - strand at 4493.825 kb on + strand at 4493.930 kb on + strand at 4494.111 kb on - strand, within Pf1N1B4_4042 at 4494.198 kb on - strand, within Pf1N1B4_4042 at 4494.198 kb on - strand, within Pf1N1B4_4042 at 4494.308 kb on + strand, within Pf1N1B4_4042 at 4494.356 kb on + strand, within Pf1N1B4_4042 at 4494.357 kb on - strand, within Pf1N1B4_4042 at 4494.402 kb on - strand, within Pf1N1B4_4042 at 4494.705 kb on - strand, within Pf1N1B4_4042 at 4494.705 kb on - strand, within Pf1N1B4_4042 at 4494.859 kb on + strand, within Pf1N1B4_4042 at 4494.860 kb on - strand, within Pf1N1B4_4042 at 4494.860 kb on - strand, within Pf1N1B4_4042 at 4494.878 kb on + strand, within Pf1N1B4_4042 at 4494.878 kb on + strand, within Pf1N1B4_4042 at 4494.879 kb on - strand, within Pf1N1B4_4042 at 4495.083 kb on - strand at 4495.187 kb on + strand at 4495.188 kb on - strand at 4495.188 kb on - strand at 4495.267 kb on + strand at 4495.267 kb on + strand at 4495.267 kb on + strand at 4495.268 kb on - strand at 4495.268 kb on - strand at 4495.268 kb on - strand at 4495.268 kb on - strand at 4495.337 kb on + strand at 4495.409 kb on + strand at 4495.409 kb on + strand at 4495.410 kb on - strand at 4495.414 kb on - strand at 4495.623 kb on - strand at 4495.635 kb on + strand at 4495.636 kb on - strand at 4495.638 kb on + strand at 4495.638 kb on + strand at 4495.638 kb on + strand at 4495.668 kb on - strand at 4495.678 kb on - strand at 4495.724 kb on + strand at 4495.724 kb on + strand at 4495.724 kb on + strand at 4495.725 kb on - strand at 4495.837 kb on + strand, within Pf1N1B4_4043 at 4495.837 kb on + strand at 4495.838 kb on - strand, within Pf1N1B4_4043 at 4495.875 kb on + strand, within Pf1N1B4_4043 at 4495.922 kb on + strand, within Pf1N1B4_4043 at 4495.974 kb on - strand, within Pf1N1B4_4043 at 4496.154 kb on + strand, within Pf1N1B4_4043 at 4496.155 kb on - strand, within Pf1N1B4_4043
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,492,853 - Pf1N1B4_4041 0.18 -0.9 4,492,853 - Pf1N1B4_4041 0.18 -1.9 4,492,853 - Pf1N1B4_4041 0.18 -1.4 4,492,879 - Pf1N1B4_4041 0.24 -0.0 4,492,994 - Pf1N1B4_4041 0.52 -0.4 4,492,994 - Pf1N1B4_4041 0.52 +0.6 4,493,035 - Pf1N1B4_4041 0.62 -0.4 4,493,035 - Pf1N1B4_4041 0.62 +1.2 4,493,035 - Pf1N1B4_4041 0.62 -0.9 4,493,284 + -0.4 4,493,326 - -0.4 4,493,329 + -1.1 4,493,330 - +0.6 4,493,345 + -0.1 4,493,406 + -0.8 4,493,406 + -0.4 4,493,406 + -0.4 4,493,425 - +1.0 4,493,433 + -0.4 4,493,433 + -0.9 4,493,433 + +0.1 4,493,433 + -1.9 4,493,433 + +0.6 4,493,433 + -1.8 4,493,433 + -0.7 4,493,433 + -0.7 4,493,433 + +1.2 4,493,433 + +0.1 4,493,433 + -0.8 4,493,434 - -1.4 4,493,434 - -0.4 4,493,434 - -0.4 4,493,434 - -1.4 4,493,434 - +0.2 4,493,513 + -0.9 4,493,513 + -2.7 4,493,513 + -0.4 4,493,513 + +0.1 4,493,514 - -0.6 4,493,514 - -2.7 4,493,514 - +0.1 4,493,514 - +1.2 4,493,514 - -0.4 4,493,515 + +0.2 4,493,515 + +0.5 4,493,515 + +0.1 4,493,515 + -0.9 4,493,515 + -1.8 4,493,516 - -1.2 4,493,516 - +0.1 4,493,516 - +0.2 4,493,516 - +0.5 4,493,551 + +0.2 4,493,780 + -1.1 4,493,781 - -1.7 4,493,781 - -0.9 4,493,825 + -0.5 4,493,930 + -1.9 4,494,111 - Pf1N1B4_4042 0.20 -1.9 4,494,198 - Pf1N1B4_4042 0.27 -0.4 4,494,198 - Pf1N1B4_4042 0.27 -0.8 4,494,308 + Pf1N1B4_4042 0.35 -1.4 4,494,356 + Pf1N1B4_4042 0.39 -1.7 4,494,357 - Pf1N1B4_4042 0.39 -1.7 4,494,402 - Pf1N1B4_4042 0.42 -1.1 4,494,705 - Pf1N1B4_4042 0.65 -1.4 4,494,705 - Pf1N1B4_4042 0.65 -1.7 4,494,859 + Pf1N1B4_4042 0.77 -1.4 4,494,860 - Pf1N1B4_4042 0.77 -1.4 4,494,860 - Pf1N1B4_4042 0.77 -1.9 4,494,878 + Pf1N1B4_4042 0.78 +0.2 4,494,878 + Pf1N1B4_4042 0.78 -0.8 4,494,879 - Pf1N1B4_4042 0.78 -2.4 4,495,083 - -1.4 4,495,187 + -0.4 4,495,188 - +0.2 4,495,188 - -0.4 4,495,267 + -2.7 4,495,267 + -2.9 4,495,267 + +0.4 4,495,268 - -2.4 4,495,268 - +0.9 4,495,268 - -0.6 4,495,268 - -1.5 4,495,337 + +0.2 4,495,409 + -1.1 4,495,409 + -0.4 4,495,410 - -1.4 4,495,414 - -0.6 4,495,623 - -0.4 4,495,635 + +1.1 4,495,636 - +1.5 4,495,638 + -2.4 4,495,638 + -1.1 4,495,638 + +0.6 4,495,668 - -2.4 4,495,678 - -0.8 4,495,724 + +0.4 4,495,724 + -0.4 4,495,724 + +0.1 4,495,725 - +0.9 4,495,837 + Pf1N1B4_4043 0.22 -0.8 4,495,837 + -1.4 4,495,838 - Pf1N1B4_4043 0.23 +0.6 4,495,875 + Pf1N1B4_4043 0.27 -0.4 4,495,922 + Pf1N1B4_4043 0.34 +0.6 4,495,974 - Pf1N1B4_4043 0.40 +0.1 4,496,154 + Pf1N1B4_4043 0.64 -0.9 4,496,155 - Pf1N1B4_4043 0.64 +0.1
Or see this region's nucleotide sequence