Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3991

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3990 and Pf1N1B4_3991 are separated by 145 nucleotidesPf1N1B4_3991 and Pf1N1B4_3992 are separated by 188 nucleotides Pf1N1B4_3990 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 4,434,460 to 4,435,359 _3990 Pf1N1B4_3991 - Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family, at 4,435,505 to 4,435,906 _3991 Pf1N1B4_3992 - Transcriptional regulator, LysR family, at 4,436,095 to 4,437,024 _3992 Position (kb) 4435 4436Strain fitness (log2 ratio) -3 -2 -1 0 1at 4434.701 kb on + strand, within Pf1N1B4_3990at 4434.702 kb on - strand, within Pf1N1B4_3990at 4434.702 kb on - strandat 4434.702 kb on - strand, within Pf1N1B4_3990at 4434.729 kb on + strand, within Pf1N1B4_3990at 4435.225 kb on + strand, within Pf1N1B4_3990at 4435.225 kb on + strand, within Pf1N1B4_3990at 4435.226 kb on - strand, within Pf1N1B4_3990at 4435.226 kb on - strand, within Pf1N1B4_3990at 4435.227 kb on + strand, within Pf1N1B4_3990at 4435.229 kb on + strand, within Pf1N1B4_3990at 4435.230 kb on - strand, within Pf1N1B4_3990at 4435.340 kb on - strandat 4435.375 kb on - strandat 4435.397 kb on - strandat 4435.500 kb on + strandat 4435.500 kb on + strandat 4435.690 kb on - strand, within Pf1N1B4_3991at 4435.690 kb on - strand, within Pf1N1B4_3991at 4435.690 kb on - strand, within Pf1N1B4_3991at 4435.834 kb on - strand, within Pf1N1B4_3991at 4435.889 kb on - strandat 4435.891 kb on - strandat 4435.891 kb on - strandat 4435.891 kb on - strandat 4435.940 kb on - strandat 4435.940 kb on - strandat 4435.977 kb on + strandat 4435.977 kb on + strandat 4435.977 kb on + strandat 4435.977 kb on + strandat 4435.978 kb on - strandat 4436.020 kb on - strandat 4436.041 kb on + strandat 4436.041 kb on + strandat 4436.041 kb on + strandat 4436.097 kb on - strandat 4436.204 kb on + strandat 4436.256 kb on - strand, within Pf1N1B4_3992at 4436.256 kb on - strand, within Pf1N1B4_3992at 4436.336 kb on + strand, within Pf1N1B4_3992at 4436.337 kb on - strand, within Pf1N1B4_3992at 4436.337 kb on - strand, within Pf1N1B4_3992at 4436.399 kb on + strand, within Pf1N1B4_3992at 4436.428 kb on - strand, within Pf1N1B4_3992at 4436.451 kb on - strandat 4436.513 kb on + strand, within Pf1N1B4_3992

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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4,434,701 + Pf1N1B4_3990 0.27 +1.3
4,434,702 - Pf1N1B4_3990 0.27 -1.9
4,434,702 - -1.3
4,434,702 - Pf1N1B4_3990 0.27 -1.9
4,434,729 + Pf1N1B4_3990 0.30 -2.4
4,435,225 + Pf1N1B4_3990 0.85 -1.9
4,435,225 + Pf1N1B4_3990 0.85 -1.9
4,435,226 - Pf1N1B4_3990 0.85 -0.6
4,435,226 - Pf1N1B4_3990 0.85 +0.7
4,435,227 + Pf1N1B4_3990 0.85 -1.3
4,435,229 + Pf1N1B4_3990 0.85 -1.3
4,435,230 - Pf1N1B4_3990 0.86 -3.6
4,435,340 - -1.3
4,435,375 - +1.7
4,435,397 - -1.3
4,435,500 + -0.7
4,435,500 + -1.9
4,435,690 - Pf1N1B4_3991 0.46 +0.7
4,435,690 - Pf1N1B4_3991 0.46 +0.0
4,435,690 - Pf1N1B4_3991 0.46 +0.3
4,435,834 - Pf1N1B4_3991 0.82 +1.7
4,435,889 - -0.3
4,435,891 - -1.9
4,435,891 - +0.0
4,435,891 - +1.3
4,435,940 - +0.2
4,435,940 - +1.3
4,435,977 + +0.1
4,435,977 + -1.0
4,435,977 + +1.3
4,435,977 + -0.4
4,435,978 - -1.0
4,436,020 - -1.6
4,436,041 + +0.3
4,436,041 + +0.7
4,436,041 + -0.7
4,436,097 - +0.7
4,436,204 + -0.3
4,436,256 - Pf1N1B4_3992 0.17 -0.9
4,436,256 - Pf1N1B4_3992 0.17 -0.7
4,436,336 + Pf1N1B4_3992 0.26 +0.9
4,436,337 - Pf1N1B4_3992 0.26 -1.1
4,436,337 - Pf1N1B4_3992 0.26 +0.3
4,436,399 + Pf1N1B4_3992 0.33 +1.3
4,436,428 - Pf1N1B4_3992 0.36 -0.3
4,436,451 - +0.7
4,436,513 + Pf1N1B4_3992 0.45 -0.2

Or see this region's nucleotide sequence