Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3989 and Pf1N1B4_3990 are separated by 781 nucleotides Pf1N1B4_3990 and Pf1N1B4_3991 are separated by 145 nucleotides Pf1N1B4_3991 and Pf1N1B4_3992 are separated by 188 nucleotides
Pf1N1B4_3989 - small molecule metabolism, at 4,432,617 to 4,433,678
_3989
Pf1N1B4_3990 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 4,434,460 to 4,435,359
_3990
Pf1N1B4_3991 - Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family, at 4,435,505 to 4,435,906
_3991
Pf1N1B4_3992 - Transcriptional regulator, LysR family, at 4,436,095 to 4,437,024
_3992
Position (kb)
4434
4435
4436 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4433.552 kb on + strand, within Pf1N1B4_3989 at 4433.670 kb on + strand at 4433.693 kb on - strand at 4433.711 kb on + strand at 4433.817 kb on + strand at 4433.818 kb on - strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.901 kb on + strand at 4433.902 kb on - strand at 4433.902 kb on - strand at 4433.902 kb on - strand at 4433.902 kb on - strand at 4433.956 kb on - strand at 4433.971 kb on - strand at 4433.971 kb on - strand at 4433.971 kb on - strand at 4434.118 kb on - strand at 4434.118 kb on - strand at 4434.128 kb on - strand at 4434.133 kb on + strand at 4434.133 kb on + strand at 4434.134 kb on - strand at 4434.134 kb on - strand at 4434.134 kb on - strand at 4434.134 kb on - strand at 4434.235 kb on + strand at 4434.236 kb on - strand at 4434.236 kb on - strand at 4434.250 kb on - strand at 4434.257 kb on - strand at 4434.701 kb on + strand, within Pf1N1B4_3990 at 4434.702 kb on - strand, within Pf1N1B4_3990 at 4434.702 kb on - strand at 4434.702 kb on - strand, within Pf1N1B4_3990 at 4434.729 kb on + strand, within Pf1N1B4_3990 at 4435.225 kb on + strand, within Pf1N1B4_3990 at 4435.225 kb on + strand, within Pf1N1B4_3990 at 4435.226 kb on - strand, within Pf1N1B4_3990 at 4435.226 kb on - strand, within Pf1N1B4_3990 at 4435.227 kb on + strand, within Pf1N1B4_3990 at 4435.229 kb on + strand, within Pf1N1B4_3990 at 4435.230 kb on - strand, within Pf1N1B4_3990 at 4435.340 kb on - strand at 4435.375 kb on - strand at 4435.397 kb on - strand at 4435.500 kb on + strand at 4435.500 kb on + strand at 4435.690 kb on - strand, within Pf1N1B4_3991 at 4435.690 kb on - strand, within Pf1N1B4_3991 at 4435.690 kb on - strand, within Pf1N1B4_3991 at 4435.834 kb on - strand, within Pf1N1B4_3991 at 4435.889 kb on - strand at 4435.891 kb on - strand at 4435.891 kb on - strand at 4435.891 kb on - strand at 4435.940 kb on - strand at 4435.940 kb on - strand at 4435.977 kb on + strand at 4435.977 kb on + strand at 4435.977 kb on + strand at 4435.977 kb on + strand at 4435.978 kb on - strand at 4436.020 kb on - strand at 4436.041 kb on + strand at 4436.041 kb on + strand at 4436.041 kb on + strand at 4436.097 kb on - strand at 4436.204 kb on + strand at 4436.256 kb on - strand, within Pf1N1B4_3992 at 4436.256 kb on - strand, within Pf1N1B4_3992 at 4436.336 kb on + strand, within Pf1N1B4_3992 at 4436.337 kb on - strand, within Pf1N1B4_3992 at 4436.337 kb on - strand, within Pf1N1B4_3992
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,433,552 + Pf1N1B4_3989 0.88 +0.3 4,433,670 + -0.7 4,433,693 - +0.9 4,433,711 + +1.7 4,433,817 + -0.0 4,433,818 - -0.8 4,433,901 + -0.4 4,433,901 + -1.3 4,433,901 + -1.3 4,433,901 + +1.0 4,433,901 + -0.9 4,433,901 + +0.6 4,433,901 + +1.3 4,433,901 + +0.3 4,433,901 + -0.1 4,433,901 + +0.7 4,433,901 + -1.3 4,433,902 - +1.9 4,433,902 - +1.0 4,433,902 - -0.4 4,433,902 - +1.3 4,433,956 - -0.3 4,433,971 - -0.3 4,433,971 - +0.7 4,433,971 - +0.0 4,434,118 - +1.7 4,434,118 - -0.6 4,434,128 - -0.5 4,434,133 + +0.3 4,434,133 + -0.7 4,434,134 - +2.0 4,434,134 - +1.0 4,434,134 - +0.1 4,434,134 - +0.3 4,434,235 + +0.1 4,434,236 - +0.7 4,434,236 - -2.6 4,434,250 - -0.3 4,434,257 - -0.7 4,434,701 + Pf1N1B4_3990 0.27 +1.3 4,434,702 - Pf1N1B4_3990 0.27 -1.9 4,434,702 - -1.3 4,434,702 - Pf1N1B4_3990 0.27 -1.9 4,434,729 + Pf1N1B4_3990 0.30 -2.4 4,435,225 + Pf1N1B4_3990 0.85 -1.9 4,435,225 + Pf1N1B4_3990 0.85 -1.9 4,435,226 - Pf1N1B4_3990 0.85 -0.6 4,435,226 - Pf1N1B4_3990 0.85 +0.7 4,435,227 + Pf1N1B4_3990 0.85 -1.3 4,435,229 + Pf1N1B4_3990 0.85 -1.3 4,435,230 - Pf1N1B4_3990 0.86 -3.6 4,435,340 - -1.3 4,435,375 - +1.7 4,435,397 - -1.3 4,435,500 + -0.7 4,435,500 + -1.9 4,435,690 - Pf1N1B4_3991 0.46 +0.7 4,435,690 - Pf1N1B4_3991 0.46 +0.0 4,435,690 - Pf1N1B4_3991 0.46 +0.3 4,435,834 - Pf1N1B4_3991 0.82 +1.7 4,435,889 - -0.3 4,435,891 - -1.9 4,435,891 - +0.0 4,435,891 - +1.3 4,435,940 - +0.2 4,435,940 - +1.3 4,435,977 + +0.1 4,435,977 + -1.0 4,435,977 + +1.3 4,435,977 + -0.4 4,435,978 - -1.0 4,436,020 - -1.6 4,436,041 + +0.3 4,436,041 + +0.7 4,436,041 + -0.7 4,436,097 - +0.7 4,436,204 + -0.3 4,436,256 - Pf1N1B4_3992 0.17 -0.9 4,436,256 - Pf1N1B4_3992 0.17 -0.7 4,436,336 + Pf1N1B4_3992 0.26 +0.9 4,436,337 - Pf1N1B4_3992 0.26 -1.1 4,436,337 - Pf1N1B4_3992 0.26 +0.3
Or see this region's nucleotide sequence