Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3917 and Pf1N1B4_3918 are separated by 256 nucleotides Pf1N1B4_3918 and Pf1N1B4_3919 are separated by 206 nucleotides
Pf1N1B4_3917 - NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13), at 4,354,679 to 4,356,127
_3917
Pf1N1B4_3918 - Thiamin biosynthesis lipoprotein ApbE, at 4,356,384 to 4,357,328
_3918
Pf1N1B4_3919 - Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1), at 4,357,535 to 4,358,929
_3919
Position (kb)
4356
4357
4358 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4355.397 kb on + strand, within Pf1N1B4_3917 at 4355.642 kb on + strand at 4355.642 kb on + strand, within Pf1N1B4_3917 at 4355.642 kb on + strand, within Pf1N1B4_3917 at 4355.643 kb on - strand, within Pf1N1B4_3917 at 4355.879 kb on + strand, within Pf1N1B4_3917 at 4355.879 kb on + strand, within Pf1N1B4_3917 at 4355.880 kb on - strand, within Pf1N1B4_3917 at 4355.888 kb on + strand, within Pf1N1B4_3917 at 4355.888 kb on + strand, within Pf1N1B4_3917 at 4355.928 kb on - strand, within Pf1N1B4_3917 at 4356.032 kb on + strand at 4356.032 kb on + strand at 4356.033 kb on - strand at 4356.260 kb on - strand at 4356.272 kb on + strand at 4356.350 kb on - strand at 4356.421 kb on - strand at 4356.432 kb on + strand at 4356.432 kb on + strand at 4356.432 kb on + strand at 4356.433 kb on - strand at 4356.433 kb on - strand at 4356.433 kb on - strand at 4356.433 kb on - strand at 4356.433 kb on - strand at 4356.554 kb on + strand, within Pf1N1B4_3918 at 4356.554 kb on + strand, within Pf1N1B4_3918 at 4356.892 kb on - strand at 4357.021 kb on - strand, within Pf1N1B4_3918 at 4357.049 kb on + strand, within Pf1N1B4_3918 at 4357.049 kb on + strand, within Pf1N1B4_3918 at 4357.050 kb on - strand, within Pf1N1B4_3918 at 4357.050 kb on - strand, within Pf1N1B4_3918 at 4357.050 kb on - strand, within Pf1N1B4_3918 at 4357.050 kb on - strand, within Pf1N1B4_3918 at 4357.237 kb on + strand at 4357.237 kb on + strand at 4357.237 kb on + strand at 4357.237 kb on + strand at 4357.237 kb on + strand at 4357.237 kb on + strand at 4357.237 kb on + strand at 4357.237 kb on + strand at 4357.237 kb on + strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.238 kb on - strand at 4357.361 kb on + strand at 4357.361 kb on + strand at 4357.361 kb on + strand at 4357.361 kb on + strand at 4357.361 kb on + strand at 4357.362 kb on - strand at 4357.376 kb on + strand at 4357.381 kb on + strand at 4357.382 kb on - strand at 4357.423 kb on + strand at 4357.423 kb on + strand at 4357.423 kb on + strand at 4357.423 kb on + strand at 4357.423 kb on + strand at 4357.423 kb on + strand at 4357.423 kb on + strand at 4357.423 kb on + strand at 4357.424 kb on - strand at 4357.424 kb on - strand at 4357.530 kb on + strand at 4357.544 kb on + strand at 4357.545 kb on - strand at 4357.545 kb on - strand at 4357.957 kb on + strand, within Pf1N1B4_3919 at 4358.021 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,355,397 + Pf1N1B4_3917 0.50 -1.9 4,355,642 + -2.3 4,355,642 + Pf1N1B4_3917 0.66 -1.3 4,355,642 + Pf1N1B4_3917 0.66 -0.3 4,355,643 - Pf1N1B4_3917 0.67 -1.6 4,355,879 + Pf1N1B4_3917 0.83 -2.3 4,355,879 + Pf1N1B4_3917 0.83 -2.3 4,355,880 - Pf1N1B4_3917 0.83 -1.3 4,355,888 + Pf1N1B4_3917 0.83 -2.7 4,355,888 + Pf1N1B4_3917 0.83 -2.9 4,355,928 - Pf1N1B4_3917 0.86 -0.3 4,356,032 + -2.3 4,356,032 + -1.9 4,356,033 - -1.3 4,356,260 - +0.3 4,356,272 + -0.6 4,356,350 - +0.0 4,356,421 - -0.3 4,356,432 + +1.0 4,356,432 + -0.3 4,356,432 + +0.0 4,356,433 - -1.0 4,356,433 - -1.1 4,356,433 - -0.6 4,356,433 - +0.7 4,356,433 - -1.1 4,356,554 + Pf1N1B4_3918 0.18 -0.8 4,356,554 + Pf1N1B4_3918 0.18 +1.3 4,356,892 - +0.7 4,357,021 - Pf1N1B4_3918 0.67 +0.3 4,357,049 + Pf1N1B4_3918 0.70 -0.3 4,357,049 + Pf1N1B4_3918 0.70 +0.7 4,357,050 - Pf1N1B4_3918 0.70 +0.7 4,357,050 - Pf1N1B4_3918 0.70 +0.4 4,357,050 - Pf1N1B4_3918 0.70 +0.1 4,357,050 - Pf1N1B4_3918 0.70 -0.3 4,357,237 + -0.5 4,357,237 + +2.0 4,357,237 + +0.5 4,357,237 + +0.3 4,357,237 + +0.3 4,357,237 + -1.9 4,357,237 + +0.3 4,357,237 + -0.3 4,357,237 + -0.3 4,357,238 - -0.9 4,357,238 - -2.3 4,357,238 - -0.3 4,357,238 - -1.3 4,357,238 - +0.3 4,357,238 - -3.1 4,357,238 - -1.4 4,357,238 - -1.5 4,357,238 - +1.7 4,357,238 - -0.0 4,357,361 + +1.5 4,357,361 + -0.9 4,357,361 + -0.3 4,357,361 + +1.0 4,357,361 + +2.0 4,357,362 - -0.3 4,357,376 + -0.3 4,357,381 + -1.1 4,357,382 - -1.9 4,357,423 + -1.3 4,357,423 + -0.3 4,357,423 + +0.3 4,357,423 + +1.0 4,357,423 + -1.1 4,357,423 + -0.3 4,357,423 + +0.5 4,357,423 + -0.9 4,357,424 - -0.3 4,357,424 - -1.3 4,357,530 + +0.7 4,357,544 + -0.4 4,357,545 - -1.9 4,357,545 - -1.9 4,357,957 + Pf1N1B4_3919 0.30 -0.3 4,358,021 + -1.9
Or see this region's nucleotide sequence