Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3904 and Pf1N1B4_3905 are separated by 98 nucleotides Pf1N1B4_3905 and Pf1N1B4_3906 are separated by 114 nucleotides Pf1N1B4_3906 and Pf1N1B4_3907 are separated by 138 nucleotides Pf1N1B4_3907 and Pf1N1B4_3908 are separated by 141 nucleotides
Pf1N1B4_3904 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,335,300 to 4,336,451
_3904
Pf1N1B4_3905 - FIG00953423: hypothetical protein, at 4,336,550 to 4,336,786
_3905
Pf1N1B4_3906 - DNA topoisomerase I (EC 5.99.1.2), at 4,336,901 to 4,339,528
_3906
Pf1N1B4_3907 - FIG00953287: hypothetical protein, at 4,339,667 to 4,340,191
_3907
Pf1N1B4_3908 - FIG00954079: hypothetical protein, at 4,340,333 to 4,340,566
_3908
Position (kb)
4336
4337
4338
4339
4340 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4336.519 kb on - strand at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.581 kb on - strand, within Pf1N1B4_3905 at 4336.603 kb on - strand, within Pf1N1B4_3905 at 4336.603 kb on - strand, within Pf1N1B4_3905 at 4336.603 kb on - strand, within Pf1N1B4_3905 at 4336.649 kb on + strand, within Pf1N1B4_3905 at 4336.650 kb on - strand, within Pf1N1B4_3905 at 4336.650 kb on - strand at 4336.650 kb on - strand, within Pf1N1B4_3905 at 4336.835 kb on + strand at 4336.835 kb on + strand at 4336.835 kb on + strand at 4336.835 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.837 kb on + strand at 4336.839 kb on + strand at 4336.877 kb on + strand at 4336.877 kb on + strand at 4336.877 kb on + strand at 4336.877 kb on + strand at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.220 kb on + strand, within Pf1N1B4_3906 at 4339.221 kb on - strand, within Pf1N1B4_3906 at 4339.427 kb on + strand at 4339.428 kb on - strand at 4339.526 kb on + strand at 4339.526 kb on + strand at 4339.537 kb on - strand at 4339.644 kb on - strand at 4339.644 kb on - strand at 4339.644 kb on - strand at 4339.682 kb on + strand at 4339.682 kb on + strand at 4339.683 kb on - strand at 4339.683 kb on - strand at 4339.683 kb on - strand at 4339.683 kb on - strand at 4339.706 kb on + strand at 4339.707 kb on - strand at 4339.707 kb on - strand at 4339.707 kb on - strand at 4339.707 kb on - strand at 4339.707 kb on - strand at 4339.857 kb on - strand, within Pf1N1B4_3907 at 4339.928 kb on - strand, within Pf1N1B4_3907 at 4340.064 kb on + strand, within Pf1N1B4_3907 at 4340.077 kb on + strand, within Pf1N1B4_3907 at 4340.147 kb on - strand at 4340.299 kb on + strand at 4340.299 kb on + strand at 4340.299 kb on + strand at 4340.299 kb on + strand at 4340.300 kb on - strand at 4340.300 kb on - strand at 4340.300 kb on - strand at 4340.301 kb on + strand at 4340.301 kb on + strand at 4340.301 kb on + strand at 4340.301 kb on + strand at 4340.302 kb on - strand at 4340.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,336,519 - -0.7 4,336,581 - Pf1N1B4_3905 0.13 -0.9 4,336,581 - Pf1N1B4_3905 0.13 +1.1 4,336,581 - Pf1N1B4_3905 0.13 -0.8 4,336,581 - Pf1N1B4_3905 0.13 -0.9 4,336,581 - Pf1N1B4_3905 0.13 -0.3 4,336,603 - Pf1N1B4_3905 0.22 +0.7 4,336,603 - Pf1N1B4_3905 0.22 +0.3 4,336,603 - Pf1N1B4_3905 0.22 -1.9 4,336,649 + Pf1N1B4_3905 0.42 -0.3 4,336,650 - Pf1N1B4_3905 0.42 -1.5 4,336,650 - -1.3 4,336,650 - Pf1N1B4_3905 0.42 -1.1 4,336,835 + +0.9 4,336,835 + +0.4 4,336,835 + -1.3 4,336,835 + +0.7 4,336,837 + -1.0 4,336,837 + -0.3 4,336,837 + +0.7 4,336,837 + +0.4 4,336,837 + +0.5 4,336,837 + -0.3 4,336,837 + -2.6 4,336,839 + +0.1 4,336,877 + -1.3 4,336,877 + +0.9 4,336,877 + +0.0 4,336,877 + +0.0 4,339,220 + Pf1N1B4_3906 0.88 -2.6 4,339,220 + Pf1N1B4_3906 0.88 -1.3 4,339,220 + Pf1N1B4_3906 0.88 -3.3 4,339,220 + Pf1N1B4_3906 0.88 -1.3 4,339,220 + Pf1N1B4_3906 0.88 -3.1 4,339,220 + Pf1N1B4_3906 0.88 -2.7 4,339,220 + -1.3 4,339,220 + Pf1N1B4_3906 0.88 -0.3 4,339,220 + Pf1N1B4_3906 0.88 -4.6 4,339,221 - Pf1N1B4_3906 0.88 -1.9 4,339,427 + -1.9 4,339,428 - -2.9 4,339,526 + -0.9 4,339,526 + +2.3 4,339,537 - +0.3 4,339,644 - -1.9 4,339,644 - -0.3 4,339,644 - +0.3 4,339,682 + +0.4 4,339,682 + -1.3 4,339,683 - -0.8 4,339,683 - -0.9 4,339,683 - -0.7 4,339,683 - -1.3 4,339,706 + +0.7 4,339,707 - -1.7 4,339,707 - -1.9 4,339,707 - -1.3 4,339,707 - -0.7 4,339,707 - +0.7 4,339,857 - Pf1N1B4_3907 0.36 -0.3 4,339,928 - Pf1N1B4_3907 0.50 -0.3 4,340,064 + Pf1N1B4_3907 0.76 +0.3 4,340,077 + Pf1N1B4_3907 0.78 -0.1 4,340,147 - -0.5 4,340,299 + +0.1 4,340,299 + -0.3 4,340,299 + +1.3 4,340,299 + -0.3 4,340,300 - -2.3 4,340,300 - -0.3 4,340,300 - -1.9 4,340,301 + +0.3 4,340,301 + -0.3 4,340,301 + +0.5 4,340,301 + +0.3 4,340,302 - -0.7 4,340,302 - +0.7
Or see this region's nucleotide sequence