Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3848 and Pf1N1B4_3849 are separated by 357 nucleotides Pf1N1B4_3849 and Pf1N1B4_3850 are separated by 179 nucleotides
Pf1N1B4_3848 - Benzoate transport protein, at 4,268,837 to 4,270,027
_3848
Pf1N1B4_3849 - Transcriptional regulator, GntR family, at 4,270,385 to 4,271,149
_3849
Pf1N1B4_3850 - Guanine deaminase (EC 3.5.4.3), at 4,271,329 to 4,272,633
_3850
Position (kb)
4270
4271
4272 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4269.452 kb on - strand, within Pf1N1B4_3848 at 4269.499 kb on - strand, within Pf1N1B4_3848 at 4269.528 kb on + strand, within Pf1N1B4_3848 at 4269.528 kb on + strand, within Pf1N1B4_3848 at 4269.528 kb on + strand, within Pf1N1B4_3848 at 4269.529 kb on - strand, within Pf1N1B4_3848 at 4269.529 kb on - strand, within Pf1N1B4_3848 at 4269.529 kb on - strand, within Pf1N1B4_3848 at 4269.707 kb on + strand, within Pf1N1B4_3848 at 4269.707 kb on + strand, within Pf1N1B4_3848 at 4269.707 kb on + strand, within Pf1N1B4_3848 at 4269.707 kb on + strand, within Pf1N1B4_3848 at 4269.707 kb on + strand, within Pf1N1B4_3848 at 4269.707 kb on + strand, within Pf1N1B4_3848 at 4269.707 kb on + strand, within Pf1N1B4_3848 at 4269.707 kb on + strand at 4269.708 kb on - strand, within Pf1N1B4_3848 at 4269.708 kb on - strand, within Pf1N1B4_3848 at 4269.708 kb on - strand, within Pf1N1B4_3848 at 4269.708 kb on - strand, within Pf1N1B4_3848 at 4269.708 kb on - strand, within Pf1N1B4_3848 at 4269.708 kb on - strand, within Pf1N1B4_3848 at 4269.708 kb on - strand, within Pf1N1B4_3848 at 4269.709 kb on + strand, within Pf1N1B4_3848 at 4269.709 kb on + strand, within Pf1N1B4_3848 at 4269.709 kb on + strand, within Pf1N1B4_3848 at 4269.709 kb on + strand, within Pf1N1B4_3848 at 4269.709 kb on + strand, within Pf1N1B4_3848 at 4269.709 kb on + strand, within Pf1N1B4_3848 at 4269.711 kb on + strand, within Pf1N1B4_3848 at 4269.711 kb on + strand, within Pf1N1B4_3848 at 4269.711 kb on + strand, within Pf1N1B4_3848 at 4269.711 kb on + strand, within Pf1N1B4_3848 at 4269.712 kb on - strand, within Pf1N1B4_3848 at 4269.712 kb on - strand, within Pf1N1B4_3848 at 4269.712 kb on - strand, within Pf1N1B4_3848 at 4270.045 kb on + strand at 4270.045 kb on + strand at 4270.045 kb on + strand at 4270.045 kb on + strand at 4270.046 kb on - strand at 4270.046 kb on - strand at 4270.208 kb on - strand at 4270.235 kb on + strand at 4270.259 kb on + strand at 4270.287 kb on + strand at 4270.287 kb on + strand at 4270.288 kb on - strand at 4270.288 kb on - strand at 4270.313 kb on - strand at 4270.337 kb on + strand at 4270.337 kb on + strand at 4270.338 kb on - strand at 4270.338 kb on - strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.340 kb on + strand at 4270.341 kb on - strand at 4270.341 kb on - strand at 4270.341 kb on - strand at 4270.341 kb on - strand at 4270.341 kb on - strand at 4270.459 kb on + strand at 4270.459 kb on + strand at 4270.459 kb on + strand at 4270.467 kb on - strand, within Pf1N1B4_3849 at 4270.467 kb on - strand, within Pf1N1B4_3849 at 4270.474 kb on + strand, within Pf1N1B4_3849 at 4270.474 kb on + strand, within Pf1N1B4_3849 at 4270.474 kb on + strand at 4270.474 kb on + strand, within Pf1N1B4_3849 at 4270.475 kb on - strand, within Pf1N1B4_3849 at 4270.475 kb on - strand, within Pf1N1B4_3849 at 4270.922 kb on + strand, within Pf1N1B4_3849 at 4270.922 kb on + strand, within Pf1N1B4_3849 at 4270.922 kb on + strand, within Pf1N1B4_3849 at 4270.952 kb on + strand, within Pf1N1B4_3849 at 4270.952 kb on + strand, within Pf1N1B4_3849 at 4270.953 kb on - strand, within Pf1N1B4_3849 at 4270.953 kb on - strand, within Pf1N1B4_3849 at 4271.360 kb on + strand at 4271.360 kb on + strand at 4271.360 kb on + strand at 4271.361 kb on - strand at 4271.361 kb on - strand at 4271.361 kb on - strand at 4271.361 kb on - strand at 4271.401 kb on + strand at 4271.401 kb on + strand at 4271.402 kb on - strand at 4271.402 kb on - strand at 4271.402 kb on - strand at 4271.402 kb on - strand at 4271.444 kb on + strand at 4271.469 kb on - strand, within Pf1N1B4_3850 at 4271.533 kb on + strand, within Pf1N1B4_3850 at 4271.533 kb on + strand, within Pf1N1B4_3850 at 4271.534 kb on - strand, within Pf1N1B4_3850 at 4271.564 kb on + strand, within Pf1N1B4_3850 at 4271.600 kb on - strand, within Pf1N1B4_3850 at 4271.600 kb on - strand, within Pf1N1B4_3850 at 4271.600 kb on - strand, within Pf1N1B4_3850 at 4271.615 kb on + strand, within Pf1N1B4_3850 at 4271.616 kb on - strand, within Pf1N1B4_3850 at 4271.616 kb on - strand at 4271.853 kb on - strand, within Pf1N1B4_3850 at 4271.861 kb on + strand, within Pf1N1B4_3850 at 4271.862 kb on - strand, within Pf1N1B4_3850 at 4271.862 kb on - strand at 4271.948 kb on + strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4272.089 kb on + strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.117 kb on - strand at 4272.117 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,269,452 - Pf1N1B4_3848 0.52 -1.8 4,269,499 - Pf1N1B4_3848 0.56 -0.2 4,269,528 + Pf1N1B4_3848 0.58 +0.8 4,269,528 + Pf1N1B4_3848 0.58 +0.4 4,269,528 + Pf1N1B4_3848 0.58 +0.8 4,269,529 - Pf1N1B4_3848 0.58 -0.3 4,269,529 - Pf1N1B4_3848 0.58 +0.2 4,269,529 - Pf1N1B4_3848 0.58 -1.2 4,269,707 + Pf1N1B4_3848 0.73 -0.6 4,269,707 + Pf1N1B4_3848 0.73 -1.2 4,269,707 + Pf1N1B4_3848 0.73 -0.2 4,269,707 + Pf1N1B4_3848 0.73 -0.4 4,269,707 + Pf1N1B4_3848 0.73 -0.8 4,269,707 + Pf1N1B4_3848 0.73 +0.8 4,269,707 + Pf1N1B4_3848 0.73 -1.8 4,269,707 + -0.2 4,269,708 - Pf1N1B4_3848 0.73 -0.6 4,269,708 - Pf1N1B4_3848 0.73 +0.0 4,269,708 - Pf1N1B4_3848 0.73 -1.8 4,269,708 - Pf1N1B4_3848 0.73 -0.2 4,269,708 - Pf1N1B4_3848 0.73 -0.6 4,269,708 - Pf1N1B4_3848 0.73 +0.4 4,269,708 - Pf1N1B4_3848 0.73 -1.5 4,269,709 + Pf1N1B4_3848 0.73 -0.2 4,269,709 + Pf1N1B4_3848 0.73 -1.2 4,269,709 + Pf1N1B4_3848 0.73 -2.0 4,269,709 + Pf1N1B4_3848 0.73 +1.1 4,269,709 + Pf1N1B4_3848 0.73 -0.2 4,269,709 + Pf1N1B4_3848 0.73 -1.4 4,269,711 + Pf1N1B4_3848 0.73 +0.8 4,269,711 + Pf1N1B4_3848 0.73 -0.6 4,269,711 + Pf1N1B4_3848 0.73 -0.2 4,269,711 + Pf1N1B4_3848 0.73 +0.4 4,269,712 - Pf1N1B4_3848 0.73 +0.4 4,269,712 - Pf1N1B4_3848 0.73 +0.0 4,269,712 - Pf1N1B4_3848 0.73 +1.8 4,270,045 + +0.8 4,270,045 + -1.2 4,270,045 + -1.8 4,270,045 + -0.2 4,270,046 - -0.2 4,270,046 - -0.1 4,270,208 - -2.2 4,270,235 + -0.2 4,270,259 + -1.2 4,270,287 + +1.4 4,270,287 + +0.8 4,270,288 - -1.5 4,270,288 - -0.8 4,270,313 - -0.2 4,270,337 + +0.1 4,270,337 + -1.2 4,270,338 - -1.2 4,270,338 - -0.7 4,270,340 + +1.1 4,270,340 + +0.4 4,270,340 + -0.5 4,270,340 + -0.7 4,270,340 + +0.5 4,270,340 + -0.4 4,270,341 - -1.2 4,270,341 - -0.2 4,270,341 - -1.4 4,270,341 - -0.8 4,270,341 - -3.5 4,270,459 + -0.2 4,270,459 + -0.2 4,270,459 + -0.2 4,270,467 - Pf1N1B4_3849 0.11 -1.2 4,270,467 - Pf1N1B4_3849 0.11 -1.5 4,270,474 + Pf1N1B4_3849 0.12 -1.0 4,270,474 + Pf1N1B4_3849 0.12 -1.5 4,270,474 + -0.8 4,270,474 + Pf1N1B4_3849 0.12 -0.8 4,270,475 - Pf1N1B4_3849 0.12 -0.2 4,270,475 - Pf1N1B4_3849 0.12 -2.2 4,270,922 + Pf1N1B4_3849 0.70 -1.8 4,270,922 + Pf1N1B4_3849 0.70 -1.6 4,270,922 + Pf1N1B4_3849 0.70 +0.0 4,270,952 + Pf1N1B4_3849 0.74 -0.2 4,270,952 + Pf1N1B4_3849 0.74 -1.2 4,270,953 - Pf1N1B4_3849 0.74 -1.8 4,270,953 - Pf1N1B4_3849 0.74 -0.6 4,271,360 + -0.2 4,271,360 + -0.2 4,271,360 + -0.1 4,271,361 - -0.2 4,271,361 - -1.2 4,271,361 - -0.2 4,271,361 - -2.5 4,271,401 + -0.8 4,271,401 + -0.2 4,271,402 - -0.6 4,271,402 - -1.2 4,271,402 - +1.1 4,271,402 - -0.2 4,271,444 + -0.2 4,271,469 - Pf1N1B4_3850 0.11 -0.5 4,271,533 + Pf1N1B4_3850 0.16 -1.2 4,271,533 + Pf1N1B4_3850 0.16 -0.6 4,271,534 - Pf1N1B4_3850 0.16 -1.8 4,271,564 + Pf1N1B4_3850 0.18 -0.6 4,271,600 - Pf1N1B4_3850 0.21 -1.0 4,271,600 - Pf1N1B4_3850 0.21 +0.6 4,271,600 - Pf1N1B4_3850 0.21 -1.5 4,271,615 + Pf1N1B4_3850 0.22 -0.5 4,271,616 - Pf1N1B4_3850 0.22 -0.2 4,271,616 - -0.2 4,271,853 - Pf1N1B4_3850 0.40 -1.0 4,271,861 + Pf1N1B4_3850 0.41 -0.8 4,271,862 - Pf1N1B4_3850 0.41 -0.8 4,271,862 - +0.4 4,271,948 + Pf1N1B4_3850 0.47 +1.8 4,271,949 - Pf1N1B4_3850 0.48 -2.5 4,271,949 - Pf1N1B4_3850 0.48 +0.8 4,271,949 - Pf1N1B4_3850 0.48 -0.6 4,271,949 - Pf1N1B4_3850 0.48 -0.8 4,271,949 - Pf1N1B4_3850 0.48 +0.1 4,272,089 + Pf1N1B4_3850 0.58 -0.2 4,272,090 - Pf1N1B4_3850 0.58 -0.9 4,272,090 - Pf1N1B4_3850 0.58 +0.4 4,272,090 - Pf1N1B4_3850 0.58 -1.2 4,272,117 - -0.2 4,272,117 - -1.8
Or see this region's nucleotide sequence