Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3819 and Pf1N1B4_3820 are separated by 184 nucleotides Pf1N1B4_3820 and Pf1N1B4_3821 are separated by 205 nucleotides
Pf1N1B4_3819 - Methylisocitrate lyase (EC 4.1.3.30), at 4,230,262 to 4,231,152
_3819
Pf1N1B4_3820 - 2-methylcitrate synthase (EC 2.3.3.5), at 4,231,337 to 4,232,464
_3820
Pf1N1B4_3821 - 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79), at 4,232,670 to 4,235,264
_3821
Position (kb)
4231
4232
4233 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4230.394 kb on + strand, within Pf1N1B4_3819 at 4230.394 kb on + strand, within Pf1N1B4_3819 at 4230.395 kb on - strand, within Pf1N1B4_3819 at 4230.648 kb on + strand, within Pf1N1B4_3819 at 4230.651 kb on + strand at 4230.652 kb on - strand, within Pf1N1B4_3819 at 4230.759 kb on - strand, within Pf1N1B4_3819 at 4230.853 kb on + strand at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.853 kb on + strand at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.925 kb on + strand, within Pf1N1B4_3819 at 4230.925 kb on + strand, within Pf1N1B4_3819 at 4230.926 kb on - strand, within Pf1N1B4_3819 at 4230.926 kb on - strand, within Pf1N1B4_3819 at 4230.926 kb on - strand at 4231.019 kb on - strand, within Pf1N1B4_3819 at 4231.019 kb on - strand, within Pf1N1B4_3819 at 4231.151 kb on - strand at 4231.201 kb on - strand at 4231.201 kb on - strand at 4231.229 kb on + strand at 4231.229 kb on + strand at 4231.229 kb on + strand at 4231.353 kb on + strand at 4231.353 kb on + strand at 4231.436 kb on + strand at 4231.436 kb on + strand at 4231.437 kb on - strand at 4231.541 kb on + strand, within Pf1N1B4_3820 at 4231.542 kb on - strand, within Pf1N1B4_3820 at 4231.743 kb on - strand, within Pf1N1B4_3820 at 4231.955 kb on + strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.128 kb on + strand, within Pf1N1B4_3820 at 4232.129 kb on - strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.292 kb on - strand, within Pf1N1B4_3820 at 4232.417 kb on + strand at 4232.417 kb on + strand at 4232.417 kb on + strand at 4232.418 kb on - strand at 4232.418 kb on - strand at 4232.418 kb on - strand at 4232.619 kb on + strand at 4232.620 kb on - strand at 4232.620 kb on - strand at 4232.646 kb on - strand at 4232.902 kb on + strand at 4232.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,230,394 + Pf1N1B4_3819 0.15 -2.2 4,230,394 + Pf1N1B4_3819 0.15 -0.2 4,230,395 - Pf1N1B4_3819 0.15 -2.5 4,230,648 + Pf1N1B4_3819 0.43 -1.2 4,230,651 + -2.5 4,230,652 - Pf1N1B4_3819 0.44 -0.2 4,230,759 - Pf1N1B4_3819 0.56 -0.2 4,230,853 + -0.2 4,230,853 + Pf1N1B4_3819 0.66 -3.5 4,230,853 + Pf1N1B4_3819 0.66 -2.8 4,230,853 + -2.2 4,230,853 + Pf1N1B4_3819 0.66 -0.2 4,230,854 - Pf1N1B4_3819 0.66 -3.5 4,230,854 - Pf1N1B4_3819 0.66 -0.2 4,230,854 - Pf1N1B4_3819 0.66 -2.5 4,230,854 - Pf1N1B4_3819 0.66 -1.8 4,230,854 - Pf1N1B4_3819 0.66 +0.8 4,230,925 + Pf1N1B4_3819 0.74 -1.8 4,230,925 + Pf1N1B4_3819 0.74 -1.2 4,230,926 - Pf1N1B4_3819 0.75 -3.2 4,230,926 - Pf1N1B4_3819 0.75 -2.5 4,230,926 - -1.2 4,231,019 - Pf1N1B4_3819 0.85 -1.2 4,231,019 - Pf1N1B4_3819 0.85 -2.2 4,231,151 - -1.2 4,231,201 - -0.2 4,231,201 - -1.8 4,231,229 + +0.4 4,231,229 + -0.6 4,231,229 + +1.1 4,231,353 + -3.7 4,231,353 + -1.2 4,231,436 + +0.8 4,231,436 + -2.2 4,231,437 - -0.2 4,231,541 + Pf1N1B4_3820 0.18 -0.2 4,231,542 - Pf1N1B4_3820 0.18 -2.2 4,231,743 - Pf1N1B4_3820 0.36 -1.6 4,231,955 + Pf1N1B4_3820 0.55 +1.4 4,231,956 - Pf1N1B4_3820 0.55 -1.8 4,231,956 - Pf1N1B4_3820 0.55 -1.8 4,231,956 - Pf1N1B4_3820 0.55 -2.5 4,232,126 + Pf1N1B4_3820 0.70 -4.7 4,232,126 + Pf1N1B4_3820 0.70 -3.2 4,232,126 + Pf1N1B4_3820 0.70 -0.2 4,232,126 + Pf1N1B4_3820 0.70 -2.5 4,232,126 + Pf1N1B4_3820 0.70 -1.2 4,232,126 + Pf1N1B4_3820 0.70 -1.9 4,232,126 + -2.2 4,232,126 + Pf1N1B4_3820 0.70 -4.4 4,232,126 + Pf1N1B4_3820 0.70 -3.2 4,232,127 - Pf1N1B4_3820 0.70 -2.2 4,232,127 - Pf1N1B4_3820 0.70 -2.2 4,232,127 - Pf1N1B4_3820 0.70 -2.8 4,232,127 - Pf1N1B4_3820 0.70 -2.2 4,232,127 - -0.2 4,232,127 - Pf1N1B4_3820 0.70 -1.8 4,232,128 + Pf1N1B4_3820 0.70 -2.2 4,232,129 - Pf1N1B4_3820 0.70 -1.2 4,232,276 + Pf1N1B4_3820 0.83 -1.2 4,232,276 + Pf1N1B4_3820 0.83 -3.0 4,232,276 + Pf1N1B4_3820 0.83 -1.2 4,232,276 + Pf1N1B4_3820 0.83 -2.5 4,232,276 + Pf1N1B4_3820 0.83 -1.2 4,232,276 + Pf1N1B4_3820 0.83 -4.4 4,232,276 + Pf1N1B4_3820 0.83 -2.5 4,232,276 + Pf1N1B4_3820 0.83 -2.5 4,232,277 - Pf1N1B4_3820 0.83 -3.0 4,232,277 - Pf1N1B4_3820 0.83 -2.5 4,232,277 - Pf1N1B4_3820 0.83 -0.2 4,232,292 - Pf1N1B4_3820 0.85 -3.2 4,232,417 + -2.8 4,232,417 + -1.8 4,232,417 + -3.2 4,232,418 - -0.2 4,232,418 - -2.2 4,232,418 - -0.2 4,232,619 + -1.7 4,232,620 - -3.2 4,232,620 - -1.2 4,232,646 - -1.8 4,232,902 + -0.2 4,232,903 - -2.0
Or see this region's nucleotide sequence