Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3816 and Pf1N1B4_3817 are separated by 5 nucleotides Pf1N1B4_3817 and Pf1N1B4_3818 are separated by 298 nucleotides Pf1N1B4_3818 and Pf1N1B4_3819 overlap by 4 nucleotides
Pf1N1B4_3816 - FIG139976: hypothetical protein, at 4,227,167 to 4,228,702
_3816
Pf1N1B4_3817 - FIG008443: hypothetical protein, at 4,228,708 to 4,229,244
_3817
Pf1N1B4_3818 - Propionate catabolism operon transcriptional regulator of GntR family [predicted], at 4,229,543 to 4,230,265
_3818
Pf1N1B4_3819 - Methylisocitrate lyase (EC 4.1.3.30), at 4,230,262 to 4,231,152
_3819
Position (kb)
4229
4230
4231 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4228.629 kb on + strand at 4228.709 kb on + strand at 4228.709 kb on + strand at 4228.709 kb on + strand at 4228.709 kb on + strand at 4228.710 kb on - strand at 4228.710 kb on - strand at 4228.710 kb on - strand at 4228.710 kb on - strand at 4228.719 kb on + strand at 4228.720 kb on - strand at 4228.757 kb on + strand at 4228.757 kb on + strand at 4228.757 kb on + strand at 4228.758 kb on - strand at 4228.758 kb on - strand at 4228.761 kb on + strand at 4228.761 kb on + strand at 4228.761 kb on + strand at 4228.762 kb on - strand, within Pf1N1B4_3817 at 4228.762 kb on - strand, within Pf1N1B4_3817 at 4228.867 kb on + strand, within Pf1N1B4_3817 at 4228.887 kb on + strand, within Pf1N1B4_3817 at 4228.916 kb on + strand, within Pf1N1B4_3817 at 4228.940 kb on + strand, within Pf1N1B4_3817 at 4228.940 kb on + strand, within Pf1N1B4_3817 at 4228.941 kb on - strand, within Pf1N1B4_3817 at 4229.024 kb on + strand, within Pf1N1B4_3817 at 4229.024 kb on + strand, within Pf1N1B4_3817 at 4229.024 kb on + strand, within Pf1N1B4_3817 at 4229.150 kb on + strand, within Pf1N1B4_3817 at 4229.165 kb on + strand, within Pf1N1B4_3817 at 4229.165 kb on + strand, within Pf1N1B4_3817 at 4229.244 kb on + strand at 4229.244 kb on + strand at 4229.244 kb on + strand at 4229.244 kb on + strand at 4229.311 kb on + strand at 4229.311 kb on + strand at 4229.311 kb on + strand at 4229.311 kb on + strand at 4229.317 kb on + strand at 4229.318 kb on - strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.385 kb on + strand at 4229.410 kb on + strand at 4229.412 kb on + strand at 4229.412 kb on + strand at 4229.412 kb on + strand at 4229.499 kb on + strand at 4229.542 kb on + strand at 4229.691 kb on - strand, within Pf1N1B4_3818 at 4229.816 kb on + strand, within Pf1N1B4_3818 at 4229.954 kb on + strand at 4229.954 kb on + strand, within Pf1N1B4_3818 at 4230.062 kb on + strand, within Pf1N1B4_3818 at 4230.062 kb on + strand, within Pf1N1B4_3818 at 4230.062 kb on + strand, within Pf1N1B4_3818 at 4230.063 kb on - strand, within Pf1N1B4_3818 at 4230.218 kb on + strand at 4230.218 kb on + strand at 4230.394 kb on + strand, within Pf1N1B4_3819 at 4230.394 kb on + strand, within Pf1N1B4_3819 at 4230.395 kb on - strand, within Pf1N1B4_3819 at 4230.648 kb on + strand, within Pf1N1B4_3819 at 4230.651 kb on + strand at 4230.652 kb on - strand, within Pf1N1B4_3819 at 4230.759 kb on - strand, within Pf1N1B4_3819 at 4230.853 kb on + strand at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.853 kb on + strand at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.925 kb on + strand, within Pf1N1B4_3819 at 4230.925 kb on + strand, within Pf1N1B4_3819 at 4230.926 kb on - strand, within Pf1N1B4_3819 at 4230.926 kb on - strand, within Pf1N1B4_3819 at 4230.926 kb on - strand at 4231.019 kb on - strand, within Pf1N1B4_3819 at 4231.019 kb on - strand, within Pf1N1B4_3819 at 4231.151 kb on - strand at 4231.201 kb on - strand at 4231.201 kb on - strand at 4231.229 kb on + strand at 4231.229 kb on + strand at 4231.229 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,228,629 + -1.2 4,228,709 + -0.9 4,228,709 + -0.6 4,228,709 + +1.6 4,228,709 + +1.8 4,228,710 - -0.2 4,228,710 - +0.5 4,228,710 - +0.2 4,228,710 - -1.2 4,228,719 + +0.8 4,228,720 - -1.8 4,228,757 + -0.2 4,228,757 + -0.2 4,228,757 + -0.2 4,228,758 - -2.5 4,228,758 - -2.5 4,228,761 + -2.2 4,228,761 + -0.2 4,228,761 + -0.2 4,228,762 - Pf1N1B4_3817 0.10 -0.2 4,228,762 - Pf1N1B4_3817 0.10 +2.4 4,228,867 + Pf1N1B4_3817 0.30 -1.8 4,228,887 + Pf1N1B4_3817 0.33 -0.8 4,228,916 + Pf1N1B4_3817 0.39 -0.1 4,228,940 + Pf1N1B4_3817 0.43 -1.0 4,228,940 + Pf1N1B4_3817 0.43 -1.2 4,228,941 - Pf1N1B4_3817 0.43 -0.4 4,229,024 + Pf1N1B4_3817 0.59 -0.2 4,229,024 + Pf1N1B4_3817 0.59 -0.2 4,229,024 + Pf1N1B4_3817 0.59 +0.8 4,229,150 + Pf1N1B4_3817 0.82 -3.0 4,229,165 + Pf1N1B4_3817 0.85 -0.3 4,229,165 + Pf1N1B4_3817 0.85 -0.8 4,229,244 + -0.2 4,229,244 + +0.0 4,229,244 + -0.6 4,229,244 + +1.4 4,229,311 + -2.5 4,229,311 + -0.8 4,229,311 + -0.0 4,229,311 + -1.8 4,229,317 + +0.4 4,229,318 - -1.3 4,229,371 + +0.5 4,229,371 + -2.2 4,229,371 + -1.8 4,229,371 + +1.0 4,229,371 + -0.5 4,229,371 + +1.6 4,229,385 + -1.2 4,229,410 + -0.2 4,229,412 + +0.4 4,229,412 + -0.2 4,229,412 + +0.4 4,229,499 + +0.5 4,229,542 + -1.8 4,229,691 - Pf1N1B4_3818 0.20 -2.2 4,229,816 + Pf1N1B4_3818 0.38 -1.8 4,229,954 + -0.2 4,229,954 + Pf1N1B4_3818 0.57 -0.2 4,230,062 + Pf1N1B4_3818 0.72 -1.2 4,230,062 + Pf1N1B4_3818 0.72 -0.8 4,230,062 + Pf1N1B4_3818 0.72 -2.8 4,230,063 - Pf1N1B4_3818 0.72 -2.2 4,230,218 + -0.5 4,230,218 + -1.8 4,230,394 + Pf1N1B4_3819 0.15 -2.2 4,230,394 + Pf1N1B4_3819 0.15 -0.2 4,230,395 - Pf1N1B4_3819 0.15 -2.5 4,230,648 + Pf1N1B4_3819 0.43 -1.2 4,230,651 + -2.5 4,230,652 - Pf1N1B4_3819 0.44 -0.2 4,230,759 - Pf1N1B4_3819 0.56 -0.2 4,230,853 + -0.2 4,230,853 + Pf1N1B4_3819 0.66 -3.5 4,230,853 + Pf1N1B4_3819 0.66 -2.8 4,230,853 + -2.2 4,230,853 + Pf1N1B4_3819 0.66 -0.2 4,230,854 - Pf1N1B4_3819 0.66 -3.5 4,230,854 - Pf1N1B4_3819 0.66 -0.2 4,230,854 - Pf1N1B4_3819 0.66 -2.5 4,230,854 - Pf1N1B4_3819 0.66 -1.8 4,230,854 - Pf1N1B4_3819 0.66 +0.8 4,230,925 + Pf1N1B4_3819 0.74 -1.8 4,230,925 + Pf1N1B4_3819 0.74 -1.2 4,230,926 - Pf1N1B4_3819 0.75 -3.2 4,230,926 - Pf1N1B4_3819 0.75 -2.5 4,230,926 - -1.2 4,231,019 - Pf1N1B4_3819 0.85 -1.2 4,231,019 - Pf1N1B4_3819 0.85 -2.2 4,231,151 - -1.2 4,231,201 - -0.2 4,231,201 - -1.8 4,231,229 + +0.4 4,231,229 + -0.6 4,231,229 + +1.1
Or see this region's nucleotide sequence