Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3810 and Pf1N1B4_3811 are separated by 464 nucleotides Pf1N1B4_3811 and Pf1N1B4_3812 are separated by 79 nucleotides Pf1N1B4_3812 and Pf1N1B4_3813 are separated by 174 nucleotides Pf1N1B4_3813 and Pf1N1B4_3814 are separated by 92 nucleotides
Pf1N1B4_3810 - Transcriptional regulator, LysR family, at 4,221,163 to 4,222,089
_3810
Pf1N1B4_3811 - Hydantoin racemase (EC 5.1.99.-), at 4,222,554 to 4,222,910
_3811
Pf1N1B4_3812 - Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10), at 4,222,990 to 4,223,730
_3812
Pf1N1B4_3813 - Homoserine kinase (EC 2.7.1.39), at 4,223,905 to 4,224,522
_3813
Pf1N1B4_3814 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 4,224,615 to 4,225,952
_3814
Position (kb)
4222
4223
4224 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4222.123 kb on + strand at 4222.124 kb on - strand at 4222.148 kb on + strand at 4222.149 kb on - strand at 4222.149 kb on - strand at 4222.149 kb on - strand at 4222.149 kb on - strand at 4222.156 kb on - strand at 4222.156 kb on - strand at 4222.156 kb on - strand at 4222.172 kb on - strand at 4222.207 kb on - strand at 4222.207 kb on - strand at 4222.247 kb on - strand at 4222.247 kb on - strand at 4222.247 kb on - strand at 4222.303 kb on + strand at 4222.311 kb on - strand at 4222.311 kb on - strand at 4222.325 kb on - strand at 4222.354 kb on - strand at 4222.354 kb on - strand at 4222.357 kb on + strand at 4222.357 kb on + strand at 4222.357 kb on + strand at 4222.357 kb on + strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.370 kb on + strand at 4222.403 kb on - strand at 4222.440 kb on - strand at 4222.447 kb on - strand at 4222.447 kb on - strand at 4222.556 kb on - strand at 4222.556 kb on - strand at 4222.556 kb on - strand at 4222.556 kb on - strand at 4222.556 kb on - strand at 4222.590 kb on - strand at 4222.590 kb on - strand, within Pf1N1B4_3811 at 4222.651 kb on + strand, within Pf1N1B4_3811 at 4222.652 kb on - strand, within Pf1N1B4_3811 at 4222.652 kb on - strand, within Pf1N1B4_3811 at 4222.652 kb on - strand, within Pf1N1B4_3811 at 4222.965 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.968 kb on - strand at 4222.978 kb on + strand at 4222.978 kb on + strand at 4222.978 kb on + strand at 4222.978 kb on + strand at 4222.979 kb on - strand at 4222.979 kb on - strand at 4222.991 kb on + strand at 4223.156 kb on + strand, within Pf1N1B4_3812 at 4223.156 kb on + strand, within Pf1N1B4_3812 at 4223.156 kb on + strand, within Pf1N1B4_3812 at 4223.157 kb on - strand, within Pf1N1B4_3812 at 4223.157 kb on - strand, within Pf1N1B4_3812 at 4223.157 kb on - strand, within Pf1N1B4_3812 at 4223.180 kb on + strand, within Pf1N1B4_3812 at 4223.180 kb on + strand, within Pf1N1B4_3812 at 4223.181 kb on - strand, within Pf1N1B4_3812 at 4223.229 kb on - strand, within Pf1N1B4_3812 at 4223.229 kb on - strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.406 kb on - strand, within Pf1N1B4_3812 at 4223.406 kb on - strand, within Pf1N1B4_3812 at 4223.406 kb on - strand, within Pf1N1B4_3812 at 4223.453 kb on + strand, within Pf1N1B4_3812 at 4223.454 kb on - strand, within Pf1N1B4_3812 at 4223.454 kb on - strand, within Pf1N1B4_3812 at 4223.454 kb on - strand at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.516 kb on + strand, within Pf1N1B4_3812 at 4223.516 kb on + strand, within Pf1N1B4_3812 at 4223.517 kb on - strand, within Pf1N1B4_3812 at 4223.517 kb on - strand, within Pf1N1B4_3812 at 4223.623 kb on + strand, within Pf1N1B4_3812 at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.744 kb on + strand at 4223.744 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4224.334 kb on + strand, within Pf1N1B4_3813 at 4224.334 kb on + strand, within Pf1N1B4_3813 at 4224.334 kb on + strand, within Pf1N1B4_3813 at 4224.335 kb on - strand, within Pf1N1B4_3813 at 4224.337 kb on + strand, within Pf1N1B4_3813 at 4224.364 kb on + strand, within Pf1N1B4_3813 at 4224.364 kb on + strand, within Pf1N1B4_3813 at 4224.364 kb on + strand, within Pf1N1B4_3813 at 4224.365 kb on - strand, within Pf1N1B4_3813 at 4224.365 kb on - strand, within Pf1N1B4_3813 at 4224.372 kb on + strand, within Pf1N1B4_3813 at 4224.520 kb on + strand at 4224.583 kb on - strand at 4224.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,222,123 + +0.0 4,222,124 - -2.5 4,222,148 + +1.1 4,222,149 - +0.4 4,222,149 - +0.8 4,222,149 - -0.1 4,222,149 - +0.0 4,222,156 - -1.8 4,222,156 - -1.8 4,222,156 - -2.2 4,222,172 - -0.2 4,222,207 - -1.8 4,222,207 - -0.2 4,222,247 - -2.0 4,222,247 - -2.8 4,222,247 - -0.8 4,222,303 + +0.8 4,222,311 - +0.2 4,222,311 - -1.4 4,222,325 - +0.4 4,222,354 - -1.9 4,222,354 - -0.2 4,222,357 + -0.2 4,222,357 + -0.9 4,222,357 + -0.2 4,222,357 + -0.8 4,222,358 - -1.2 4,222,358 - -1.8 4,222,358 - +0.4 4,222,358 - +0.1 4,222,358 - +1.0 4,222,358 - +0.2 4,222,358 - -0.8 4,222,358 - +0.4 4,222,358 - -0.2 4,222,358 - -0.2 4,222,358 - -0.2 4,222,358 - +0.4 4,222,358 - -0.9 4,222,370 + +2.0 4,222,403 - -0.8 4,222,440 - -0.2 4,222,447 - +1.4 4,222,447 - -0.7 4,222,556 - +0.4 4,222,556 - +0.6 4,222,556 - +0.8 4,222,556 - -0.2 4,222,556 - -0.9 4,222,590 - +0.4 4,222,590 - Pf1N1B4_3811 0.10 -0.5 4,222,651 + Pf1N1B4_3811 0.27 +0.5 4,222,652 - Pf1N1B4_3811 0.27 -2.0 4,222,652 - Pf1N1B4_3811 0.27 +1.5 4,222,652 - Pf1N1B4_3811 0.27 -1.9 4,222,965 + -0.5 4,222,967 + -0.5 4,222,967 + +1.4 4,222,967 + +0.4 4,222,967 + -0.4 4,222,967 + -1.5 4,222,967 + +2.6 4,222,967 + -2.2 4,222,967 + +0.4 4,222,967 + +1.4 4,222,967 + -0.5 4,222,968 - -1.2 4,222,978 + -0.2 4,222,978 + -0.2 4,222,978 + +1.1 4,222,978 + -1.2 4,222,979 - -0.5 4,222,979 - +0.2 4,222,991 + -1.5 4,223,156 + Pf1N1B4_3812 0.22 -1.8 4,223,156 + Pf1N1B4_3812 0.22 -1.2 4,223,156 + Pf1N1B4_3812 0.22 -1.2 4,223,157 - Pf1N1B4_3812 0.23 -2.2 4,223,157 - Pf1N1B4_3812 0.23 -3.0 4,223,157 - Pf1N1B4_3812 0.23 -0.2 4,223,180 + Pf1N1B4_3812 0.26 -1.8 4,223,180 + Pf1N1B4_3812 0.26 -2.2 4,223,181 - Pf1N1B4_3812 0.26 -1.5 4,223,229 - Pf1N1B4_3812 0.32 -1.2 4,223,229 - Pf1N1B4_3812 0.32 -2.2 4,223,405 + Pf1N1B4_3812 0.56 -0.8 4,223,405 + Pf1N1B4_3812 0.56 -1.9 4,223,405 + Pf1N1B4_3812 0.56 -3.0 4,223,405 + Pf1N1B4_3812 0.56 -1.8 4,223,405 + Pf1N1B4_3812 0.56 -1.2 4,223,405 + Pf1N1B4_3812 0.56 -2.5 4,223,406 - Pf1N1B4_3812 0.56 -2.5 4,223,406 - Pf1N1B4_3812 0.56 -3.0 4,223,406 - Pf1N1B4_3812 0.56 -3.2 4,223,453 + Pf1N1B4_3812 0.62 -1.5 4,223,454 - Pf1N1B4_3812 0.63 -0.2 4,223,454 - Pf1N1B4_3812 0.63 -1.2 4,223,454 - -0.2 4,223,486 + Pf1N1B4_3812 0.67 -3.0 4,223,486 + Pf1N1B4_3812 0.67 -2.2 4,223,486 + Pf1N1B4_3812 0.67 -2.4 4,223,486 + Pf1N1B4_3812 0.67 -1.4 4,223,486 + Pf1N1B4_3812 0.67 -0.2 4,223,486 + Pf1N1B4_3812 0.67 -3.0 4,223,486 + Pf1N1B4_3812 0.67 -1.8 4,223,487 - Pf1N1B4_3812 0.67 -2.5 4,223,487 - Pf1N1B4_3812 0.67 -3.0 4,223,487 - Pf1N1B4_3812 0.67 -1.9 4,223,487 - Pf1N1B4_3812 0.67 -0.9 4,223,487 - Pf1N1B4_3812 0.67 -0.8 4,223,487 - Pf1N1B4_3812 0.67 -2.2 4,223,487 - Pf1N1B4_3812 0.67 -1.4 4,223,487 - Pf1N1B4_3812 0.67 -2.2 4,223,487 - Pf1N1B4_3812 0.67 -0.2 4,223,487 - Pf1N1B4_3812 0.67 -2.2 4,223,487 - Pf1N1B4_3812 0.67 -2.5 4,223,516 + Pf1N1B4_3812 0.71 -2.2 4,223,516 + Pf1N1B4_3812 0.71 -4.2 4,223,517 - Pf1N1B4_3812 0.71 -0.6 4,223,517 - Pf1N1B4_3812 0.71 -2.2 4,223,623 + Pf1N1B4_3812 0.85 -1.2 4,223,690 + -1.3 4,223,690 + -1.2 4,223,690 + -1.6 4,223,690 + -3.7 4,223,690 + -3.5 4,223,690 + -2.2 4,223,690 + -1.8 4,223,690 + -1.2 4,223,690 + -3.4 4,223,690 + -2.5 4,223,691 - -2.8 4,223,691 - -2.5 4,223,691 - -1.6 4,223,691 - -2.2 4,223,691 - -2.0 4,223,691 - -3.4 4,223,744 + -0.2 4,223,744 + -3.5 4,223,769 + -3.5 4,223,769 + -0.2 4,223,769 + -0.2 4,223,769 + -1.2 4,223,769 + -2.0 4,223,769 + -3.7 4,223,769 + -0.2 4,223,769 + -2.8 4,223,769 + -1.8 4,223,769 + -1.5 4,223,769 + -2.7 4,223,769 + -2.3 4,223,769 + -2.9 4,223,769 + -2.5 4,223,769 + -1.9 4,223,769 + -1.2 4,223,769 + -3.4 4,223,770 - -0.2 4,223,770 - -0.2 4,223,770 - -1.2 4,223,770 - -0.2 4,223,770 - -1.2 4,223,770 - -0.7 4,223,770 - -0.2 4,223,771 + +0.8 4,223,771 + -0.8 4,223,771 + -3.4 4,223,771 + -1.4 4,223,771 + -0.2 4,223,771 + -3.2 4,223,771 + -3.0 4,223,771 + -3.2 4,223,771 + -0.2 4,223,771 + -2.0 4,223,771 + -3.4 4,223,771 + -3.2 4,223,771 + -2.7 4,223,771 + -4.2 4,223,771 + -1.8 4,223,771 + -1.4 4,223,771 + -2.5 4,223,771 + -0.6 4,223,771 + -4.0 4,223,771 + -1.8 4,223,771 + -3.2 4,223,771 + -2.8 4,223,771 + -1.8 4,223,771 + -2.5 4,223,771 + -0.2 4,223,771 + -1.2 4,223,771 + -2.7 4,223,771 + -1.2 4,223,771 + -0.8 4,223,771 + +1.1 4,223,771 + -1.8 4,223,771 + -2.0 4,223,771 + +0.2 4,223,771 + -2.5 4,223,771 + -2.1 4,223,771 + -2.5 4,223,771 + -1.2 4,223,772 - -1.4 4,223,772 - +1.1 4,223,772 - +1.1 4,223,772 - -2.8 4,223,772 - +1.4 4,223,772 - +0.8 4,223,772 - +0.8 4,223,772 - +0.7 4,223,772 - -0.2 4,223,772 - +0.8 4,223,772 - +1.4 4,223,772 - -0.5 4,224,334 + Pf1N1B4_3813 0.69 +1.8 4,224,334 + Pf1N1B4_3813 0.69 -0.4 4,224,334 + Pf1N1B4_3813 0.69 +0.2 4,224,335 - Pf1N1B4_3813 0.70 -0.2 4,224,337 + Pf1N1B4_3813 0.70 -1.5 4,224,364 + Pf1N1B4_3813 0.74 +1.4 4,224,364 + Pf1N1B4_3813 0.74 -1.2 4,224,364 + Pf1N1B4_3813 0.74 -0.6 4,224,365 - Pf1N1B4_3813 0.74 -1.2 4,224,365 - Pf1N1B4_3813 0.74 +1.5 4,224,372 + Pf1N1B4_3813 0.76 +0.8 4,224,520 + +0.5 4,224,583 - +0.2 4,224,656 + -0.2
Or see this region's nucleotide sequence