Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3799 and Pf1N1B4_3800 are separated by 214 nucleotides Pf1N1B4_3800 and Pf1N1B4_5229 are separated by 308 nucleotides Pf1N1B4_5229 and Pf1N1B4_3801 are separated by 321 nucleotides Pf1N1B4_3801 and Pf1N1B4_3802 are separated by 68 nucleotides
Pf1N1B4_3799 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 4,208,246 to 4,208,968
_3799
Pf1N1B4_3800 - Enoyl-CoA hydratase/isomerase family protein, at 4,209,183 to 4,209,872
_3800
Pf1N1B4_5229 - tRNA-Ser-GGA, at 4,210,181 to 4,210,267
_5229
Pf1N1B4_3801 - hypothetical protein, at 4,210,589 to 4,210,786
_3801
Pf1N1B4_3802 - PlcB, ORFX, ORFP, ORFB, ORFA, ldh gene, at 4,210,855 to 4,211,175
_3802
Position (kb)
4209
4210 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4208.202 kb on + strand at 4208.258 kb on + strand at 4208.258 kb on + strand at 4208.258 kb on + strand at 4208.259 kb on - strand at 4208.259 kb on - strand at 4208.421 kb on + strand, within Pf1N1B4_3799 at 4208.422 kb on - strand, within Pf1N1B4_3799 at 4208.422 kb on - strand, within Pf1N1B4_3799 at 4208.737 kb on - strand, within Pf1N1B4_3799 at 4208.779 kb on + strand, within Pf1N1B4_3799 at 4208.844 kb on + strand, within Pf1N1B4_3799 at 4208.845 kb on - strand, within Pf1N1B4_3799 at 4208.845 kb on - strand, within Pf1N1B4_3799 at 4208.847 kb on - strand, within Pf1N1B4_3799 at 4208.859 kb on - strand, within Pf1N1B4_3799 at 4208.927 kb on - strand at 4208.950 kb on + strand at 4209.038 kb on + strand at 4209.038 kb on + strand at 4209.081 kb on + strand at 4209.081 kb on + strand at 4209.111 kb on - strand at 4209.185 kb on - strand at 4209.191 kb on + strand at 4209.192 kb on - strand at 4209.192 kb on - strand at 4209.267 kb on - strand at 4209.548 kb on + strand, within Pf1N1B4_3800 at 4209.549 kb on - strand, within Pf1N1B4_3800 at 4209.692 kb on - strand, within Pf1N1B4_3800 at 4209.722 kb on + strand, within Pf1N1B4_3800 at 4209.723 kb on - strand, within Pf1N1B4_3800 at 4209.854 kb on - strand at 4209.888 kb on - strand at 4209.888 kb on - strand at 4209.890 kb on + strand at 4209.891 kb on - strand at 4209.891 kb on - strand at 4209.928 kb on + strand at 4209.928 kb on + strand at 4210.010 kb on + strand at 4210.010 kb on + strand at 4210.010 kb on + strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.013 kb on - strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.370 kb on + strand at 4210.370 kb on + strand at 4210.370 kb on + strand at 4210.370 kb on + strand at 4210.371 kb on - strand at 4210.371 kb on - strand at 4210.371 kb on - strand at 4210.371 kb on - strand at 4210.371 kb on - strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.400 kb on - strand at 4210.400 kb on - strand at 4210.426 kb on + strand at 4210.426 kb on + strand at 4210.426 kb on + strand at 4210.427 kb on - strand at 4210.427 kb on - strand at 4210.499 kb on + strand at 4210.499 kb on + strand at 4210.500 kb on - strand at 4210.588 kb on + strand at 4210.729 kb on + strand, within Pf1N1B4_3801 at 4210.730 kb on - strand, within Pf1N1B4_3801 at 4210.744 kb on + strand, within Pf1N1B4_3801 at 4210.745 kb on - strand, within Pf1N1B4_3801 at 4210.784 kb on + strand at 4210.785 kb on - strand at 4210.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,208,202 + -0.6 4,208,258 + +0.5 4,208,258 + -0.2 4,208,258 + -0.0 4,208,259 - +0.3 4,208,259 - +0.8 4,208,421 + Pf1N1B4_3799 0.24 +1.6 4,208,422 - Pf1N1B4_3799 0.24 +1.0 4,208,422 - Pf1N1B4_3799 0.24 -0.4 4,208,737 - Pf1N1B4_3799 0.68 +0.4 4,208,779 + Pf1N1B4_3799 0.74 -1.8 4,208,844 + Pf1N1B4_3799 0.83 +0.1 4,208,845 - Pf1N1B4_3799 0.83 -0.2 4,208,845 - Pf1N1B4_3799 0.83 -0.2 4,208,847 - Pf1N1B4_3799 0.83 +0.1 4,208,859 - Pf1N1B4_3799 0.85 -0.2 4,208,927 - -2.2 4,208,950 + -1.2 4,209,038 + -0.2 4,209,038 + +0.2 4,209,081 + +0.1 4,209,081 + -1.2 4,209,111 - -0.8 4,209,185 - -0.2 4,209,191 + -0.4 4,209,192 - -0.8 4,209,192 - -1.2 4,209,267 - -1.2 4,209,548 + Pf1N1B4_3800 0.53 +1.1 4,209,549 - Pf1N1B4_3800 0.53 -0.4 4,209,692 - Pf1N1B4_3800 0.74 -1.8 4,209,722 + Pf1N1B4_3800 0.78 +1.1 4,209,723 - Pf1N1B4_3800 0.78 +1.4 4,209,854 - +0.8 4,209,888 - -0.2 4,209,888 - -0.7 4,209,890 + +0.2 4,209,891 - +0.4 4,209,891 - -0.2 4,209,928 + -0.2 4,209,928 + -0.2 4,210,010 + -0.6 4,210,010 + +0.9 4,210,010 + -1.0 4,210,011 - +0.3 4,210,011 - -0.9 4,210,011 - -0.9 4,210,011 - -0.8 4,210,011 - -0.2 4,210,011 - +1.4 4,210,013 - -0.4 4,210,014 + -0.2 4,210,014 + +0.4 4,210,014 + -0.2 4,210,014 + -2.0 4,210,014 + -0.2 4,210,014 + +0.4 4,210,014 + -0.9 4,210,015 - -0.2 4,210,015 - +1.1 4,210,015 - -1.1 4,210,015 - -0.9 4,210,015 - +0.4 4,210,015 - +0.2 4,210,015 - -0.2 4,210,015 - -0.6 4,210,015 - +1.0 4,210,015 - -0.6 4,210,370 + +0.6 4,210,370 + -1.8 4,210,370 + -1.8 4,210,370 + -0.2 4,210,371 - +1.8 4,210,371 - -1.8 4,210,371 - +0.4 4,210,371 - -1.2 4,210,371 - -0.2 4,210,399 + -2.2 4,210,399 + +0.8 4,210,399 + -1.2 4,210,399 + -1.0 4,210,399 + -1.4 4,210,399 + -0.2 4,210,400 - +0.4 4,210,400 - +1.8 4,210,426 + +0.4 4,210,426 + +0.5 4,210,426 + -0.2 4,210,427 - -1.8 4,210,427 - -0.2 4,210,499 + -0.2 4,210,499 + -0.6 4,210,500 - -0.5 4,210,588 + -0.2 4,210,729 + Pf1N1B4_3801 0.71 -2.0 4,210,730 - Pf1N1B4_3801 0.71 -0.2 4,210,744 + Pf1N1B4_3801 0.78 -0.2 4,210,745 - Pf1N1B4_3801 0.79 -1.8 4,210,784 + -0.6 4,210,785 - -0.0 4,210,838 - -2.2
Or see this region's nucleotide sequence