Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3762 and Pf1N1B4_3763 are separated by 65 nucleotides Pf1N1B4_3763 and Pf1N1B4_3764 are separated by 120 nucleotides Pf1N1B4_3764 and Pf1N1B4_3765 are separated by 88 nucleotides Pf1N1B4_3765 and Pf1N1B4_3766 overlap by 4 nucleotides
Pf1N1B4_3762 - FIG137887: membrane protein related to purine degradation, at 4,173,333 to 4,174,562
_3762
Pf1N1B4_3763 - Ureidoglycolate hydrolase (EC 3.5.3.19), at 4,174,628 to 4,175,131
_3763
Pf1N1B4_3764 - Allantoicase (EC 3.5.3.4), at 4,175,252 to 4,176,247
_3764
Pf1N1B4_3765 - 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) decarboxylase, at 4,176,336 to 4,176,851
_3765
Pf1N1B4_3766 - Uricase (urate oxidase) (EC 1.7.3.3), at 4,176,848 to 4,177,774
_3766
Position (kb)
4175
4176
4177 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4174.282 kb on + strand, within Pf1N1B4_3762 at 4174.282 kb on + strand, within Pf1N1B4_3762 at 4174.327 kb on + strand, within Pf1N1B4_3762 at 4174.328 kb on - strand, within Pf1N1B4_3762 at 4174.328 kb on - strand, within Pf1N1B4_3762 at 4174.328 kb on - strand, within Pf1N1B4_3762 at 4174.399 kb on + strand at 4174.400 kb on - strand, within Pf1N1B4_3762 at 4174.490 kb on - strand at 4174.630 kb on - strand at 4174.630 kb on - strand at 4174.630 kb on - strand at 4174.687 kb on + strand, within Pf1N1B4_3763 at 4174.688 kb on - strand, within Pf1N1B4_3763 at 4174.773 kb on + strand, within Pf1N1B4_3763 at 4174.773 kb on + strand, within Pf1N1B4_3763 at 4174.773 kb on + strand, within Pf1N1B4_3763 at 4174.773 kb on + strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.777 kb on + strand, within Pf1N1B4_3763 at 4174.777 kb on + strand, within Pf1N1B4_3763 at 4174.777 kb on + strand, within Pf1N1B4_3763 at 4174.777 kb on + strand, within Pf1N1B4_3763 at 4174.778 kb on - strand, within Pf1N1B4_3763 at 4174.778 kb on - strand, within Pf1N1B4_3763 at 4174.778 kb on - strand, within Pf1N1B4_3763 at 4174.778 kb on - strand, within Pf1N1B4_3763 at 4175.188 kb on + strand at 4175.253 kb on + strand at 4175.253 kb on + strand at 4175.253 kb on + strand at 4175.253 kb on + strand at 4175.254 kb on - strand at 4175.254 kb on - strand at 4175.257 kb on + strand at 4175.258 kb on - strand at 4175.362 kb on + strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.488 kb on - strand, within Pf1N1B4_3764 at 4175.579 kb on - strand at 4175.845 kb on - strand, within Pf1N1B4_3764 at 4176.237 kb on + strand at 4176.237 kb on + strand at 4176.264 kb on + strand at 4176.265 kb on - strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.269 kb on - strand at 4176.339 kb on + strand at 4176.339 kb on + strand at 4176.340 kb on - strand at 4176.466 kb on - strand, within Pf1N1B4_3765 at 4176.502 kb on + strand, within Pf1N1B4_3765 at 4176.503 kb on - strand, within Pf1N1B4_3765 at 4176.503 kb on - strand, within Pf1N1B4_3765 at 4176.503 kb on - strand at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.937 kb on + strand at 4177.044 kb on + strand, within Pf1N1B4_3766 at 4177.044 kb on + strand, within Pf1N1B4_3766 at 4177.045 kb on - strand, within Pf1N1B4_3766 at 4177.144 kb on - strand at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.210 kb on - strand, within Pf1N1B4_3766 at 4177.210 kb on - strand, within Pf1N1B4_3766
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,174,282 + Pf1N1B4_3762 0.77 +1.4 4,174,282 + Pf1N1B4_3762 0.77 -0.2 4,174,327 + Pf1N1B4_3762 0.81 +0.4 4,174,328 - Pf1N1B4_3762 0.81 -0.8 4,174,328 - Pf1N1B4_3762 0.81 -2.2 4,174,328 - Pf1N1B4_3762 0.81 +1.8 4,174,399 + -0.2 4,174,400 - Pf1N1B4_3762 0.87 -1.5 4,174,490 - -0.8 4,174,630 - +2.8 4,174,630 - +0.2 4,174,630 - +1.2 4,174,687 + Pf1N1B4_3763 0.12 -0.1 4,174,688 - Pf1N1B4_3763 0.12 -1.2 4,174,773 + Pf1N1B4_3763 0.29 +2.4 4,174,773 + Pf1N1B4_3763 0.29 +2.4 4,174,773 + Pf1N1B4_3763 0.29 -1.0 4,174,773 + Pf1N1B4_3763 0.29 +1.4 4,174,774 - Pf1N1B4_3763 0.29 -1.5 4,174,774 - Pf1N1B4_3763 0.29 -0.8 4,174,774 - Pf1N1B4_3763 0.29 -0.2 4,174,774 - Pf1N1B4_3763 0.29 -0.8 4,174,774 - Pf1N1B4_3763 0.29 -0.8 4,174,774 - Pf1N1B4_3763 0.29 +1.1 4,174,777 + Pf1N1B4_3763 0.30 -0.2 4,174,777 + Pf1N1B4_3763 0.30 -1.2 4,174,777 + Pf1N1B4_3763 0.30 -1.5 4,174,777 + Pf1N1B4_3763 0.30 -1.2 4,174,778 - Pf1N1B4_3763 0.30 -2.5 4,174,778 - Pf1N1B4_3763 0.30 +1.8 4,174,778 - Pf1N1B4_3763 0.30 -0.4 4,174,778 - Pf1N1B4_3763 0.30 +0.8 4,175,188 + -1.2 4,175,253 + -2.2 4,175,253 + +0.8 4,175,253 + +0.4 4,175,253 + +1.1 4,175,254 - -1.2 4,175,254 - -1.8 4,175,257 + -0.6 4,175,258 - -1.2 4,175,362 + Pf1N1B4_3764 0.11 +1.4 4,175,363 - Pf1N1B4_3764 0.11 +1.1 4,175,363 - Pf1N1B4_3764 0.11 +0.5 4,175,363 - Pf1N1B4_3764 0.11 +1.1 4,175,363 - Pf1N1B4_3764 0.11 +0.8 4,175,363 - Pf1N1B4_3764 0.11 -0.5 4,175,488 - Pf1N1B4_3764 0.24 +0.2 4,175,579 - -0.2 4,175,845 - Pf1N1B4_3764 0.60 +0.4 4,176,237 + +0.6 4,176,237 + -0.9 4,176,264 + -3.0 4,176,265 - +1.0 4,176,268 + -0.5 4,176,268 + -1.8 4,176,268 + -0.8 4,176,268 + +0.5 4,176,269 - -0.2 4,176,339 + +0.8 4,176,339 + -2.2 4,176,340 - -0.0 4,176,466 - Pf1N1B4_3765 0.25 -1.2 4,176,502 + Pf1N1B4_3765 0.32 +0.8 4,176,503 - Pf1N1B4_3765 0.32 +0.8 4,176,503 - Pf1N1B4_3765 0.32 -0.6 4,176,503 - +2.6 4,176,778 + Pf1N1B4_3765 0.86 -0.6 4,176,778 + Pf1N1B4_3765 0.86 -1.0 4,176,778 + Pf1N1B4_3765 0.86 -0.2 4,176,778 + Pf1N1B4_3765 0.86 -0.2 4,176,778 + Pf1N1B4_3765 0.86 -1.7 4,176,778 + Pf1N1B4_3765 0.86 +1.6 4,176,778 + Pf1N1B4_3765 0.86 +0.3 4,176,778 + Pf1N1B4_3765 0.86 -1.8 4,176,778 + Pf1N1B4_3765 0.86 -0.2 4,176,779 - Pf1N1B4_3765 0.86 -0.8 4,176,779 - Pf1N1B4_3765 0.86 +0.0 4,176,779 - Pf1N1B4_3765 0.86 -0.4 4,176,779 - Pf1N1B4_3765 0.86 +0.1 4,176,779 - Pf1N1B4_3765 0.86 -0.2 4,176,937 + +0.4 4,177,044 + Pf1N1B4_3766 0.21 -0.2 4,177,044 + Pf1N1B4_3766 0.21 -2.5 4,177,045 - Pf1N1B4_3766 0.21 -2.5 4,177,144 - -0.2 4,177,209 + Pf1N1B4_3766 0.39 -1.2 4,177,209 + Pf1N1B4_3766 0.39 +0.1 4,177,209 + Pf1N1B4_3766 0.39 -0.2 4,177,210 - Pf1N1B4_3766 0.39 -1.1 4,177,210 - Pf1N1B4_3766 0.39 +0.8
Or see this region's nucleotide sequence