Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3720 and Pf1N1B4_3721 are separated by 146 nucleotides Pf1N1B4_3721 and Pf1N1B4_3722 are separated by 274 nucleotides
Pf1N1B4_3720 - Long-chain fatty acid transport protein, at 4,126,740 to 4,128,008
_3720
Pf1N1B4_3721 - FIG00956435: hypothetical protein, at 4,128,155 to 4,128,637
_3721
Pf1N1B4_3722 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,128,912 to 4,129,136
_3722
Position (kb)
4128
4129
4130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4127.998 kb on - strand at 4128.012 kb on + strand at 4128.013 kb on - strand at 4128.044 kb on - strand at 4128.435 kb on + strand, within Pf1N1B4_3721 at 4128.435 kb on + strand, within Pf1N1B4_3721 at 4128.436 kb on - strand, within Pf1N1B4_3721 at 4128.583 kb on - strand, within Pf1N1B4_3721 at 4128.606 kb on + strand at 4128.668 kb on + strand at 4128.753 kb on - strand at 4128.753 kb on - strand at 4128.936 kb on - strand, within Pf1N1B4_3722 at 4128.974 kb on - strand, within Pf1N1B4_3722 at 4128.999 kb on - strand, within Pf1N1B4_3722 at 4128.999 kb on - strand, within Pf1N1B4_3722 at 4129.136 kb on + strand at 4129.136 kb on + strand at 4129.136 kb on + strand at 4129.137 kb on - strand at 4129.137 kb on - strand at 4129.137 kb on - strand at 4129.137 kb on - strand at 4129.137 kb on - strand at 4129.137 kb on - strand at 4129.137 kb on - strand at 4129.137 kb on - strand at 4129.149 kb on + strand at 4129.150 kb on - strand at 4129.150 kb on - strand at 4129.182 kb on - strand at 4129.182 kb on - strand at 4129.288 kb on - strand at 4129.288 kb on - strand at 4129.288 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.292 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.296 kb on - strand at 4129.381 kb on - strand at 4129.381 kb on - strand at 4129.381 kb on - strand at 4129.543 kb on + strand at 4129.544 kb on - strand at 4129.544 kb on - strand at 4130.031 kb on - strand at 4130.081 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,127,998 - -1.2 4,128,012 + +0.5 4,128,013 - -0.5 4,128,044 - +1.1 4,128,435 + Pf1N1B4_3721 0.58 +1.8 4,128,435 + Pf1N1B4_3721 0.58 +0.8 4,128,436 - Pf1N1B4_3721 0.58 -0.8 4,128,583 - Pf1N1B4_3721 0.89 +0.4 4,128,606 + -1.2 4,128,668 + +0.2 4,128,753 - -0.8 4,128,753 - -0.2 4,128,936 - Pf1N1B4_3722 0.11 +0.5 4,128,974 - Pf1N1B4_3722 0.28 +1.3 4,128,999 - Pf1N1B4_3722 0.39 +1.4 4,128,999 - Pf1N1B4_3722 0.39 -0.3 4,129,136 + -0.5 4,129,136 + -0.5 4,129,136 + -0.2 4,129,137 - -1.5 4,129,137 - +0.8 4,129,137 - -0.2 4,129,137 - +2.6 4,129,137 - +1.1 4,129,137 - -0.9 4,129,137 - -0.8 4,129,137 - -0.2 4,129,149 + +0.8 4,129,150 - -1.2 4,129,150 - +1.4 4,129,182 - -0.2 4,129,182 - +1.4 4,129,288 - -1.6 4,129,288 - +0.6 4,129,288 - -1.6 4,129,292 - -0.2 4,129,292 - -0.8 4,129,292 - -0.6 4,129,292 - -0.5 4,129,292 - -1.2 4,129,292 - -0.2 4,129,292 - +0.5 4,129,292 - -0.8 4,129,292 - +1.4 4,129,292 - +0.5 4,129,296 - -2.0 4,129,296 - +0.2 4,129,296 - +0.6 4,129,296 - -1.4 4,129,296 - -1.6 4,129,296 - -0.2 4,129,296 - +0.2 4,129,296 - -0.5 4,129,296 - -2.4 4,129,296 - +0.8 4,129,296 - -0.2 4,129,381 - +0.5 4,129,381 - -1.5 4,129,381 - -0.2 4,129,543 + -0.2 4,129,544 - +0.2 4,129,544 - -0.6 4,130,031 - -1.0 4,130,081 - -0.2
Or see this region's nucleotide sequence