Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3604 and Pf1N1B4_3605 overlap by 8 nucleotides Pf1N1B4_3605 and Pf1N1B4_3606 are separated by 7 nucleotides Pf1N1B4_3606 and Pf1N1B4_3607 overlap by 11 nucleotides
Pf1N1B4_3604 - Flagellar M-ring protein FliF, at 4,014,467 to 4,016,254
_3604
Pf1N1B4_3605 - Flagellar motor switch protein FliG, at 4,016,247 to 4,017,266
_3605
Pf1N1B4_3606 - Flagellar assembly protein FliH, at 4,017,274 to 4,018,083
_3606
Pf1N1B4_3607 - Flagellum-specific ATP synthase FliI, at 4,018,073 to 4,019,431
_3607
Position (kb) 4016
4017
4018 Strain fitness (log2 ratio) -2
-1
0
1 at 4015.263 kb on + strand, within Pf1N1B4_3604 at 4015.263 kb on + strand, within Pf1N1B4_3604 at 4015.264 kb on - strand, within Pf1N1B4_3604 at 4015.301 kb on + strand, within Pf1N1B4_3604 at 4015.301 kb on + strand, within Pf1N1B4_3604 at 4015.303 kb on + strand, within Pf1N1B4_3604 at 4015.911 kb on - strand, within Pf1N1B4_3604 at 4015.911 kb on - strand, within Pf1N1B4_3604 at 4016.493 kb on + strand, within Pf1N1B4_3605 at 4016.493 kb on + strand, within Pf1N1B4_3605 at 4016.671 kb on - strand, within Pf1N1B4_3605 at 4016.671 kb on - strand at 4016.910 kb on - strand, within Pf1N1B4_3605 at 4017.209 kb on + strand at 4017.264 kb on + strand at 4017.264 kb on + strand at 4017.264 kb on + strand at 4017.264 kb on + strand at 4017.264 kb on + strand at 4017.265 kb on - strand at 4017.265 kb on - strand at 4017.265 kb on - strand at 4017.265 kb on - strand at 4017.265 kb on - strand at 4017.273 kb on + strand at 4017.274 kb on - strand at 4017.274 kb on - strand at 4017.274 kb on - strand at 4017.274 kb on - strand at 4017.484 kb on + strand, within Pf1N1B4_3606 at 4017.484 kb on + strand, within Pf1N1B4_3606 at 4017.484 kb on + strand, within Pf1N1B4_3606 at 4017.485 kb on - strand, within Pf1N1B4_3606 at 4017.485 kb on - strand at 4017.784 kb on + strand, within Pf1N1B4_3606 at 4017.784 kb on + strand, within Pf1N1B4_3606 at 4017.784 kb on + strand, within Pf1N1B4_3606 at 4017.784 kb on + strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4017.785 kb on - strand, within Pf1N1B4_3606 at 4018.042 kb on - strand at 4018.115 kb on + strand at 4018.250 kb on + strand, within Pf1N1B4_3607 at 4018.251 kb on - strand, within Pf1N1B4_3607
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,015,263 + Pf1N1B4_3604 0.45 -0.2 4,015,263 + Pf1N1B4_3604 0.45 +0.5 4,015,264 - Pf1N1B4_3604 0.45 -1.2 4,015,301 + Pf1N1B4_3604 0.47 +0.4 4,015,301 + Pf1N1B4_3604 0.47 -1.5 4,015,303 + Pf1N1B4_3604 0.47 +1.1 4,015,911 - Pf1N1B4_3604 0.81 -1.8 4,015,911 - Pf1N1B4_3604 0.81 +0.8 4,016,493 + Pf1N1B4_3605 0.24 +1.4 4,016,493 + Pf1N1B4_3605 0.24 -1.2 4,016,671 - Pf1N1B4_3605 0.42 +0.8 4,016,671 - -0.2 4,016,910 - Pf1N1B4_3605 0.65 +0.2 4,017,209 + -2.5 4,017,264 + -0.2 4,017,264 + -0.2 4,017,264 + +0.4 4,017,264 + +0.8 4,017,264 + +1.4 4,017,265 - -0.2 4,017,265 - -0.2 4,017,265 - +0.8 4,017,265 - -1.8 4,017,265 - +0.1 4,017,273 + +0.5 4,017,274 - +0.4 4,017,274 - -0.2 4,017,274 - -0.2 4,017,274 - +0.4 4,017,484 + Pf1N1B4_3606 0.26 -0.2 4,017,484 + Pf1N1B4_3606 0.26 -0.6 4,017,484 + Pf1N1B4_3606 0.26 -0.9 4,017,485 - Pf1N1B4_3606 0.26 -0.6 4,017,485 - -0.2 4,017,784 + Pf1N1B4_3606 0.63 +0.8 4,017,784 + Pf1N1B4_3606 0.63 +0.1 4,017,784 + Pf1N1B4_3606 0.63 -2.4 4,017,784 + Pf1N1B4_3606 0.63 -0.6 4,017,785 - Pf1N1B4_3606 0.63 +0.8 4,017,785 - Pf1N1B4_3606 0.63 -1.2 4,017,785 - Pf1N1B4_3606 0.63 -0.8 4,017,785 - Pf1N1B4_3606 0.63 -0.2 4,017,785 - Pf1N1B4_3606 0.63 +0.5 4,017,785 - Pf1N1B4_3606 0.63 -0.9 4,017,785 - Pf1N1B4_3606 0.63 +0.8 4,017,785 - Pf1N1B4_3606 0.63 -0.2 4,017,785 - Pf1N1B4_3606 0.63 +0.4 4,017,785 - Pf1N1B4_3606 0.63 +0.8 4,018,042 - +0.2 4,018,115 + -0.5 4,018,250 + Pf1N1B4_3607 0.13 +0.8 4,018,251 - Pf1N1B4_3607 0.13 +1.2
Or see this region's nucleotide sequence