Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3512 and Pf1N1B4_3513 are separated by 11 nucleotides Pf1N1B4_3513 and Pf1N1B4_3514 are separated by 247 nucleotides Pf1N1B4_3514 and Pf1N1B4_3515 overlap by 4 nucleotides Pf1N1B4_3515 and Pf1N1B4_3516 are separated by 1 nucleotides
Pf1N1B4_3512 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASI (EC 2.3.1.41), at 3,918,597 to 3,919,817
_3512
Pf1N1B4_3513 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabA form (EC 4.2.1.59), at 3,919,829 to 3,920,344
_3513
Pf1N1B4_3514 - Sensor histidine kinase/response regulator, at 3,920,592 to 3,922,493
_3514
Pf1N1B4_3515 - FIG00953467: hypothetical protein, at 3,922,490 to 3,923,422
_3515
Pf1N1B4_3516 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 3,923,424 to 3,925,547
_3516
Position (kb)
3920
3921
3922
3923 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3920.500 kb on - strand at 3920.537 kb on - strand at 3920.537 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.841 kb on + strand, within Pf1N1B4_3514 at 3920.842 kb on - strand, within Pf1N1B4_3514 at 3920.842 kb on - strand, within Pf1N1B4_3514 at 3920.842 kb on - strand, within Pf1N1B4_3514 at 3920.876 kb on + strand, within Pf1N1B4_3514 at 3920.877 kb on - strand, within Pf1N1B4_3514 at 3920.877 kb on - strand, within Pf1N1B4_3514 at 3920.877 kb on - strand, within Pf1N1B4_3514 at 3920.975 kb on + strand, within Pf1N1B4_3514 at 3921.011 kb on + strand, within Pf1N1B4_3514 at 3921.011 kb on + strand, within Pf1N1B4_3514 at 3921.011 kb on + strand, within Pf1N1B4_3514 at 3921.011 kb on + strand, within Pf1N1B4_3514 at 3921.012 kb on - strand, within Pf1N1B4_3514 at 3921.012 kb on - strand, within Pf1N1B4_3514 at 3921.012 kb on - strand, within Pf1N1B4_3514 at 3921.012 kb on - strand, within Pf1N1B4_3514 at 3921.012 kb on - strand, within Pf1N1B4_3514 at 3921.013 kb on + strand, within Pf1N1B4_3514 at 3921.013 kb on + strand, within Pf1N1B4_3514 at 3921.014 kb on - strand, within Pf1N1B4_3514 at 3921.014 kb on - strand, within Pf1N1B4_3514 at 3921.014 kb on - strand, within Pf1N1B4_3514 at 3921.014 kb on - strand, within Pf1N1B4_3514 at 3921.014 kb on - strand, within Pf1N1B4_3514 at 3921.014 kb on - strand, within Pf1N1B4_3514 at 3921.014 kb on - strand, within Pf1N1B4_3514 at 3921.014 kb on - strand, within Pf1N1B4_3514 at 3921.152 kb on + strand, within Pf1N1B4_3514 at 3921.153 kb on - strand at 3921.415 kb on - strand, within Pf1N1B4_3514 at 3921.488 kb on + strand at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.488 kb on + strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.489 kb on - strand, within Pf1N1B4_3514 at 3921.862 kb on + strand, within Pf1N1B4_3514 at 3921.863 kb on - strand, within Pf1N1B4_3514 at 3921.953 kb on + strand, within Pf1N1B4_3514 at 3921.954 kb on - strand, within Pf1N1B4_3514 at 3921.954 kb on - strand, within Pf1N1B4_3514 at 3921.954 kb on - strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.057 kb on + strand, within Pf1N1B4_3514 at 3922.058 kb on - strand, within Pf1N1B4_3514 at 3922.058 kb on - strand, within Pf1N1B4_3514 at 3922.058 kb on - strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.198 kb on + strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.199 kb on - strand, within Pf1N1B4_3514 at 3922.227 kb on + strand, within Pf1N1B4_3514 at 3922.227 kb on + strand at 3922.227 kb on + strand, within Pf1N1B4_3514 at 3922.228 kb on - strand, within Pf1N1B4_3514 at 3922.242 kb on + strand, within Pf1N1B4_3514 at 3922.242 kb on + strand, within Pf1N1B4_3514 at 3922.243 kb on - strand, within Pf1N1B4_3514 at 3922.397 kb on + strand at 3922.398 kb on - strand at 3922.463 kb on + strand at 3922.552 kb on + strand at 3922.583 kb on + strand at 3922.583 kb on + strand at 3922.584 kb on - strand, within Pf1N1B4_3515 at 3922.584 kb on - strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.683 kb on + strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.684 kb on - strand, within Pf1N1B4_3515 at 3922.687 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.752 kb on + strand, within Pf1N1B4_3515 at 3922.791 kb on + strand, within Pf1N1B4_3515 at 3922.792 kb on - strand, within Pf1N1B4_3515 at 3923.165 kb on + strand, within Pf1N1B4_3515 at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.425 kb on + strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.426 kb on - strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.449 kb on + strand at 3923.450 kb on - strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.470 kb on + strand at 3923.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,920,500 - -1.5 3,920,537 - +0.5 3,920,537 - +0.0 3,920,594 - -0.9 3,920,594 - -0.6 3,920,594 - +0.0 3,920,594 - +0.6 3,920,594 - -1.5 3,920,594 - -0.4 3,920,594 - +0.2 3,920,594 - -0.7 3,920,841 + Pf1N1B4_3514 0.13 -0.5 3,920,842 - Pf1N1B4_3514 0.13 +0.3 3,920,842 - Pf1N1B4_3514 0.13 +1.2 3,920,842 - Pf1N1B4_3514 0.13 +1.4 3,920,876 + Pf1N1B4_3514 0.15 +0.8 3,920,877 - Pf1N1B4_3514 0.15 +0.1 3,920,877 - Pf1N1B4_3514 0.15 +0.7 3,920,877 - Pf1N1B4_3514 0.15 +0.9 3,920,975 + Pf1N1B4_3514 0.20 +0.6 3,921,011 + Pf1N1B4_3514 0.22 +0.6 3,921,011 + Pf1N1B4_3514 0.22 +1.4 3,921,011 + Pf1N1B4_3514 0.22 +2.6 3,921,011 + Pf1N1B4_3514 0.22 +0.8 3,921,012 - Pf1N1B4_3514 0.22 +1.0 3,921,012 - Pf1N1B4_3514 0.22 -0.6 3,921,012 - Pf1N1B4_3514 0.22 +0.6 3,921,012 - Pf1N1B4_3514 0.22 -0.6 3,921,012 - Pf1N1B4_3514 0.22 +0.1 3,921,013 + Pf1N1B4_3514 0.22 -0.8 3,921,013 + Pf1N1B4_3514 0.22 +0.7 3,921,014 - Pf1N1B4_3514 0.22 +0.3 3,921,014 - Pf1N1B4_3514 0.22 +0.1 3,921,014 - Pf1N1B4_3514 0.22 +1.4 3,921,014 - Pf1N1B4_3514 0.22 +0.3 3,921,014 - Pf1N1B4_3514 0.22 +1.1 3,921,014 - Pf1N1B4_3514 0.22 -0.7 3,921,014 - Pf1N1B4_3514 0.22 +0.8 3,921,014 - Pf1N1B4_3514 0.22 +0.5 3,921,152 + Pf1N1B4_3514 0.29 +0.9 3,921,153 - +0.8 3,921,415 - Pf1N1B4_3514 0.43 +1.2 3,921,488 + -0.2 3,921,488 + Pf1N1B4_3514 0.47 +1.4 3,921,488 + Pf1N1B4_3514 0.47 +1.3 3,921,488 + Pf1N1B4_3514 0.47 +1.3 3,921,488 + Pf1N1B4_3514 0.47 +1.0 3,921,488 + Pf1N1B4_3514 0.47 +0.8 3,921,488 + Pf1N1B4_3514 0.47 -0.2 3,921,488 + Pf1N1B4_3514 0.47 +0.7 3,921,488 + Pf1N1B4_3514 0.47 +3.2 3,921,488 + Pf1N1B4_3514 0.47 +1.8 3,921,488 + Pf1N1B4_3514 0.47 +0.6 3,921,489 - Pf1N1B4_3514 0.47 +2.6 3,921,489 - Pf1N1B4_3514 0.47 +0.2 3,921,489 - Pf1N1B4_3514 0.47 -0.8 3,921,489 - Pf1N1B4_3514 0.47 +0.3 3,921,489 - Pf1N1B4_3514 0.47 +0.9 3,921,489 - Pf1N1B4_3514 0.47 +0.8 3,921,489 - Pf1N1B4_3514 0.47 -0.2 3,921,489 - Pf1N1B4_3514 0.47 +1.6 3,921,489 - Pf1N1B4_3514 0.47 +1.4 3,921,862 + Pf1N1B4_3514 0.67 -0.2 3,921,863 - Pf1N1B4_3514 0.67 -0.8 3,921,953 + Pf1N1B4_3514 0.72 +1.1 3,921,954 - Pf1N1B4_3514 0.72 +0.4 3,921,954 - Pf1N1B4_3514 0.72 +1.8 3,921,954 - Pf1N1B4_3514 0.72 +0.2 3,922,057 + Pf1N1B4_3514 0.77 +0.6 3,922,057 + Pf1N1B4_3514 0.77 +0.8 3,922,057 + Pf1N1B4_3514 0.77 +0.6 3,922,057 + Pf1N1B4_3514 0.77 +2.4 3,922,057 + Pf1N1B4_3514 0.77 +0.5 3,922,058 - Pf1N1B4_3514 0.77 -0.9 3,922,058 - Pf1N1B4_3514 0.77 +1.8 3,922,058 - Pf1N1B4_3514 0.77 +0.2 3,922,198 + Pf1N1B4_3514 0.84 +0.8 3,922,198 + Pf1N1B4_3514 0.84 +1.0 3,922,198 + Pf1N1B4_3514 0.84 +0.7 3,922,198 + Pf1N1B4_3514 0.84 -1.8 3,922,198 + Pf1N1B4_3514 0.84 +0.8 3,922,198 + Pf1N1B4_3514 0.84 +2.8 3,922,198 + Pf1N1B4_3514 0.84 +2.6 3,922,198 + Pf1N1B4_3514 0.84 +1.2 3,922,198 + Pf1N1B4_3514 0.84 -0.2 3,922,199 - Pf1N1B4_3514 0.84 -0.2 3,922,199 - Pf1N1B4_3514 0.84 +0.3 3,922,199 - Pf1N1B4_3514 0.84 +0.1 3,922,199 - Pf1N1B4_3514 0.84 +1.4 3,922,199 - Pf1N1B4_3514 0.84 +2.4 3,922,227 + Pf1N1B4_3514 0.86 -0.4 3,922,227 + +0.4 3,922,227 + Pf1N1B4_3514 0.86 +1.0 3,922,228 - Pf1N1B4_3514 0.86 +0.8 3,922,242 + Pf1N1B4_3514 0.87 +1.8 3,922,242 + Pf1N1B4_3514 0.87 +0.8 3,922,243 - Pf1N1B4_3514 0.87 +0.9 3,922,397 + +1.8 3,922,398 - +0.4 3,922,463 + +1.1 3,922,552 + +0.2 3,922,583 + +0.1 3,922,583 + -0.5 3,922,584 - Pf1N1B4_3515 0.10 -3.0 3,922,584 - Pf1N1B4_3515 0.10 +0.8 3,922,683 + Pf1N1B4_3515 0.21 +0.8 3,922,683 + Pf1N1B4_3515 0.21 +0.4 3,922,683 + Pf1N1B4_3515 0.21 -1.8 3,922,683 + Pf1N1B4_3515 0.21 -0.2 3,922,683 + Pf1N1B4_3515 0.21 +1.6 3,922,684 - Pf1N1B4_3515 0.21 -3.0 3,922,684 - Pf1N1B4_3515 0.21 -1.8 3,922,684 - Pf1N1B4_3515 0.21 -0.8 3,922,687 + Pf1N1B4_3515 0.21 +1.1 3,922,752 + Pf1N1B4_3515 0.28 +0.7 3,922,752 + Pf1N1B4_3515 0.28 +0.9 3,922,752 + Pf1N1B4_3515 0.28 +0.8 3,922,752 + Pf1N1B4_3515 0.28 +0.6 3,922,752 + Pf1N1B4_3515 0.28 +0.8 3,922,791 + Pf1N1B4_3515 0.32 +2.2 3,922,792 - Pf1N1B4_3515 0.32 -1.2 3,923,165 + Pf1N1B4_3515 0.72 -0.8 3,923,425 + +1.6 3,923,425 + +1.3 3,923,425 + +1.8 3,923,425 + +0.6 3,923,425 + +0.2 3,923,425 + +0.1 3,923,425 + +0.8 3,923,425 + +0.1 3,923,426 - +0.4 3,923,426 - +0.5 3,923,426 - +1.4 3,923,426 - +1.8 3,923,449 + -0.2 3,923,449 + +0.8 3,923,449 + +0.1 3,923,449 + +1.4 3,923,450 - -1.2 3,923,470 + +0.4 3,923,470 + -2.5 3,923,470 + +0.8 3,923,470 + +1.4 3,923,470 + +0.9 3,923,471 - -1.2
Or see this region's nucleotide sequence