Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3505 and Pf1N1B4_3506 are separated by 114 nucleotides Pf1N1B4_3506 and Pf1N1B4_3507 overlap by 1 nucleotides
Pf1N1B4_3505 - FIG074102: hypothetical protein, at 3,910,593 to 3,911,615
_3505
Pf1N1B4_3506 - Cysteine synthase B (EC 2.5.1.47), at 3,911,730 to 3,912,632
_3506
Pf1N1B4_3507 - 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939, at 3,912,632 to 3,913,984
_3507
Position (kb)
3911
3912
3913 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3910.731 kb on + strand, within Pf1N1B4_3505 at 3910.731 kb on + strand, within Pf1N1B4_3505 at 3910.731 kb on + strand, within Pf1N1B4_3505 at 3910.731 kb on + strand, within Pf1N1B4_3505 at 3910.731 kb on + strand, within Pf1N1B4_3505 at 3910.732 kb on - strand, within Pf1N1B4_3505 at 3910.732 kb on - strand, within Pf1N1B4_3505 at 3910.732 kb on - strand, within Pf1N1B4_3505 at 3910.732 kb on - strand, within Pf1N1B4_3505 at 3910.732 kb on - strand, within Pf1N1B4_3505 at 3910.732 kb on - strand, within Pf1N1B4_3505 at 3910.732 kb on - strand, within Pf1N1B4_3505 at 3910.732 kb on - strand, within Pf1N1B4_3505 at 3910.733 kb on + strand, within Pf1N1B4_3505 at 3910.829 kb on + strand, within Pf1N1B4_3505 at 3910.863 kb on + strand, within Pf1N1B4_3505 at 3910.863 kb on + strand, within Pf1N1B4_3505 at 3910.864 kb on - strand, within Pf1N1B4_3505 at 3910.872 kb on + strand, within Pf1N1B4_3505 at 3910.872 kb on + strand, within Pf1N1B4_3505 at 3910.872 kb on + strand, within Pf1N1B4_3505 at 3910.872 kb on + strand, within Pf1N1B4_3505 at 3910.872 kb on + strand, within Pf1N1B4_3505 at 3910.873 kb on - strand, within Pf1N1B4_3505 at 3910.873 kb on - strand, within Pf1N1B4_3505 at 3910.873 kb on - strand, within Pf1N1B4_3505 at 3910.873 kb on - strand, within Pf1N1B4_3505 at 3910.950 kb on - strand, within Pf1N1B4_3505 at 3910.974 kb on + strand, within Pf1N1B4_3505 at 3910.975 kb on - strand, within Pf1N1B4_3505 at 3911.017 kb on + strand, within Pf1N1B4_3505 at 3911.017 kb on + strand, within Pf1N1B4_3505 at 3911.017 kb on + strand, within Pf1N1B4_3505 at 3911.116 kb on + strand, within Pf1N1B4_3505 at 3911.130 kb on + strand, within Pf1N1B4_3505 at 3911.130 kb on + strand, within Pf1N1B4_3505 at 3911.131 kb on - strand, within Pf1N1B4_3505 at 3911.131 kb on - strand, within Pf1N1B4_3505 at 3911.131 kb on - strand, within Pf1N1B4_3505 at 3911.318 kb on + strand at 3911.330 kb on - strand at 3911.342 kb on + strand, within Pf1N1B4_3505 at 3911.367 kb on + strand, within Pf1N1B4_3505 at 3911.388 kb on + strand, within Pf1N1B4_3505 at 3911.388 kb on + strand, within Pf1N1B4_3505 at 3911.389 kb on - strand at 3911.484 kb on + strand, within Pf1N1B4_3505 at 3911.554 kb on + strand at 3911.554 kb on + strand at 3911.554 kb on + strand at 3911.555 kb on - strand at 3911.580 kb on + strand at 3911.580 kb on + strand at 3911.580 kb on + strand at 3911.724 kb on + strand at 3911.724 kb on + strand at 3911.995 kb on - strand, within Pf1N1B4_3506 at 3912.038 kb on + strand, within Pf1N1B4_3506 at 3912.192 kb on + strand, within Pf1N1B4_3506 at 3912.192 kb on + strand, within Pf1N1B4_3506 at 3912.192 kb on + strand, within Pf1N1B4_3506 at 3912.193 kb on - strand at 3912.197 kb on + strand, within Pf1N1B4_3506 at 3912.197 kb on + strand, within Pf1N1B4_3506 at 3912.294 kb on + strand, within Pf1N1B4_3506 at 3912.294 kb on + strand, within Pf1N1B4_3506 at 3912.387 kb on + strand, within Pf1N1B4_3506 at 3912.387 kb on + strand, within Pf1N1B4_3506 at 3912.387 kb on + strand, within Pf1N1B4_3506 at 3912.387 kb on + strand, within Pf1N1B4_3506 at 3912.387 kb on + strand, within Pf1N1B4_3506 at 3912.388 kb on - strand at 3912.402 kb on + strand, within Pf1N1B4_3506 at 3912.402 kb on + strand, within Pf1N1B4_3506 at 3912.402 kb on + strand, within Pf1N1B4_3506 at 3912.403 kb on - strand, within Pf1N1B4_3506 at 3912.403 kb on - strand, within Pf1N1B4_3506 at 3912.403 kb on - strand, within Pf1N1B4_3506 at 3912.460 kb on + strand, within Pf1N1B4_3506 at 3912.460 kb on + strand, within Pf1N1B4_3506 at 3912.460 kb on + strand, within Pf1N1B4_3506 at 3912.461 kb on - strand, within Pf1N1B4_3506 at 3912.597 kb on + strand at 3912.597 kb on + strand at 3912.597 kb on + strand at 3912.597 kb on + strand at 3913.179 kb on + strand, within Pf1N1B4_3507 at 3913.179 kb on + strand, within Pf1N1B4_3507 at 3913.179 kb on + strand, within Pf1N1B4_3507
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,910,731 + Pf1N1B4_3505 0.13 +0.4 3,910,731 + Pf1N1B4_3505 0.13 -1.6 3,910,731 + Pf1N1B4_3505 0.13 -1.6 3,910,731 + Pf1N1B4_3505 0.13 +1.8 3,910,731 + Pf1N1B4_3505 0.13 +1.4 3,910,732 - Pf1N1B4_3505 0.14 -1.7 3,910,732 - Pf1N1B4_3505 0.14 +0.4 3,910,732 - Pf1N1B4_3505 0.14 -0.2 3,910,732 - Pf1N1B4_3505 0.14 -0.2 3,910,732 - Pf1N1B4_3505 0.14 +0.4 3,910,732 - Pf1N1B4_3505 0.14 -0.7 3,910,732 - Pf1N1B4_3505 0.14 +0.6 3,910,732 - Pf1N1B4_3505 0.14 -0.7 3,910,733 + Pf1N1B4_3505 0.14 -0.5 3,910,829 + Pf1N1B4_3505 0.23 +0.8 3,910,863 + Pf1N1B4_3505 0.26 -0.6 3,910,863 + Pf1N1B4_3505 0.26 -0.7 3,910,864 - Pf1N1B4_3505 0.26 -1.2 3,910,872 + Pf1N1B4_3505 0.27 +0.3 3,910,872 + Pf1N1B4_3505 0.27 -1.7 3,910,872 + Pf1N1B4_3505 0.27 +0.6 3,910,872 + Pf1N1B4_3505 0.27 -0.2 3,910,872 + Pf1N1B4_3505 0.27 -1.3 3,910,873 - Pf1N1B4_3505 0.27 -0.6 3,910,873 - Pf1N1B4_3505 0.27 +0.2 3,910,873 - Pf1N1B4_3505 0.27 -2.0 3,910,873 - Pf1N1B4_3505 0.27 -0.2 3,910,950 - Pf1N1B4_3505 0.35 +0.8 3,910,974 + Pf1N1B4_3505 0.37 -1.3 3,910,975 - Pf1N1B4_3505 0.37 +1.4 3,911,017 + Pf1N1B4_3505 0.41 +0.8 3,911,017 + Pf1N1B4_3505 0.41 +1.3 3,911,017 + Pf1N1B4_3505 0.41 +0.3 3,911,116 + Pf1N1B4_3505 0.51 -0.4 3,911,130 + Pf1N1B4_3505 0.52 -0.9 3,911,130 + Pf1N1B4_3505 0.52 -0.3 3,911,131 - Pf1N1B4_3505 0.53 -0.2 3,911,131 - Pf1N1B4_3505 0.53 -0.7 3,911,131 - Pf1N1B4_3505 0.53 -2.2 3,911,318 + -0.2 3,911,330 - -0.2 3,911,342 + Pf1N1B4_3505 0.73 -2.2 3,911,367 + Pf1N1B4_3505 0.76 +0.8 3,911,388 + Pf1N1B4_3505 0.78 +0.3 3,911,388 + Pf1N1B4_3505 0.78 +1.1 3,911,389 - +0.4 3,911,484 + Pf1N1B4_3505 0.87 +0.1 3,911,554 + -1.2 3,911,554 + +1.2 3,911,554 + -2.2 3,911,555 - +0.8 3,911,580 + -1.2 3,911,580 + +0.2 3,911,580 + -0.4 3,911,724 + -0.9 3,911,724 + -1.6 3,911,995 - Pf1N1B4_3506 0.29 -1.8 3,912,038 + Pf1N1B4_3506 0.34 -3.0 3,912,192 + Pf1N1B4_3506 0.51 -2.5 3,912,192 + Pf1N1B4_3506 0.51 -3.6 3,912,192 + Pf1N1B4_3506 0.51 -1.8 3,912,193 - -1.8 3,912,197 + Pf1N1B4_3506 0.52 -0.2 3,912,197 + Pf1N1B4_3506 0.52 -3.3 3,912,294 + Pf1N1B4_3506 0.62 -1.2 3,912,294 + Pf1N1B4_3506 0.62 -2.0 3,912,387 + Pf1N1B4_3506 0.73 -1.2 3,912,387 + Pf1N1B4_3506 0.73 -1.8 3,912,387 + Pf1N1B4_3506 0.73 -2.5 3,912,387 + Pf1N1B4_3506 0.73 -1.8 3,912,387 + Pf1N1B4_3506 0.73 -2.2 3,912,388 - -1.2 3,912,402 + Pf1N1B4_3506 0.74 -2.5 3,912,402 + Pf1N1B4_3506 0.74 -3.2 3,912,402 + Pf1N1B4_3506 0.74 -0.2 3,912,403 - Pf1N1B4_3506 0.75 -2.5 3,912,403 - Pf1N1B4_3506 0.75 -1.8 3,912,403 - Pf1N1B4_3506 0.75 -0.2 3,912,460 + Pf1N1B4_3506 0.81 -3.3 3,912,460 + Pf1N1B4_3506 0.81 -2.2 3,912,460 + Pf1N1B4_3506 0.81 -3.9 3,912,461 - Pf1N1B4_3506 0.81 -1.8 3,912,597 + -1.8 3,912,597 + -3.8 3,912,597 + -1.5 3,912,597 + -3.2 3,913,179 + Pf1N1B4_3507 0.40 +0.0 3,913,179 + Pf1N1B4_3507 0.40 -1.2 3,913,179 + Pf1N1B4_3507 0.40 +0.1
Or see this region's nucleotide sequence